Oral anaphylaxis to peanut in a mouse model is associated with gut permeability but not with Tlr4 or Dock8 mutations

Gertie, Jake A.; Zhang, Biyan; Liu, Elise G.; Hoyt, Laura R.; Yin, Xiangyun; Xu, Lan; Long, Lauren L.; Soldatenko, Arielle; Gowthaman, Uthaman; Williams, Adam; Eisenbarth, Stephanie C.

doi:10.1016/j.jaci.2021.05.015

Cited by 20 publications

(15 citation statements)

References 43 publications

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“…Whether this phenomena reflects antibody‐mediated facilitated absorption is unclear. Allergen absorption kinetics may be a key determinant of severity in a murine model of peanut anaphylaxis 142 ; thus, further in‐human studies are warranted.…”

Section: Methodsmentioning

confidence: 99%

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Risk factors for severe reactions in food allergy: Rapid evidence review with meta‐analysis

Turner

Arasi

Ballmer‐Weber

et al. 2022

Allergy

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This rapid review summarizes the most up to date evidence about the risk factors for severe food-induced allergic reactions. We searched three bibliographic databases for studies published between January 2010 and August 2021. We included 88 studies and synthesized the evidence narratively, undertaking meta-analysis where appropriate. Significant uncertainties remain with respect to the prediction of severe reactions, both anaphylaxis and/or severe anaphylaxis refractory to treatment. Prior anaphylaxis, an asthma diagnosis, IgE sensitization or basophil activation tests are not good predictors. Some molecular allergology markers may be helpful. Hospital presentations for anaphylaxis are highest in young children, yet this age group appears at lower risk of severe outcomes. Risk of severe outcomes is greatest in adolescence and young adulthood, but the contribution of risk taking behaviour in contributing to severe outcomes is unclear. Evidence for an impact of cofactors on severity is lacking,

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Section: Methodsmentioning

confidence: 99%

“…Allergen absorption kinetics may be a key determinant of severity in a murine model of peanut anaphylaxis 142 ; thus, further in-human studies are warranted.…”

Section: Evidencementioning

confidence: 99%

Risk factors for severe reactions in food allergy: Rapid evidence review with meta‐analysis

Turner

Arasi

Ballmer‐Weber

et al. 2022

Allergy

View full text Add to dashboard Cite

show abstract

“…The kinetics of allergen presence in the blood are remarkably similar between these data and our own results using egg protein in mice. 4 Although we readily detect food-specific IgA in the stool of allergic mice, we have not observed food-specific IgE by ELISA; however, it is possible that our assays are not sensitive enough to detect gut IgE. The role of specific-allergen absorption mediated by IgE is compelling and merits further study.…”

Section: To the Editormentioning

confidence: 75%

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Hoyt

Williams

Eisenbarth

2022

Journal of Allergy and Clinical Immunology

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“…In addition, the healthy mice showed oral tolerance to food antigens, while allergy reactions persisted in the TLR4-deficient mice, indicating that intestinal microbes play an important role in food tolerance through the TLR4 signaling pathway ( 35 ). However, C3H/HeJ mice with corrected TLR4 alleles were sensitized and challenged with peanuts in a study ( 36 ), revealing that mice were resistant to anaphylaxis after the oral peanut challenge; restoring TLR4 function in mice did not protect them from anaphylaxis. A similar conclusion was obtained in Berin’s study, which revealed that TLR4-deficient mice did not show Th2 immune advantage and food allergic reaction ( 37 ).…”

Section: Discussionmentioning

confidence: 99%

Bifidobacterium animalis KV9 and Lactobacillus vaginalis FN3 alleviated β-lactoglobulin-induced allergy by modulating dendritic cells in mice

Tian

Fan

et al. 2022

Front. Immunol.

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Food allergy is a serious public health problem because of its high incidence and risk. Probiotics can induce immune regulation in patients with allergic diseases, but its mechanism is not fully clear. In this paper, β-lactoglobulin (β-LG)-sensitized mice were used as models to explore the mechanism of Bifidobacterium animalis KV9 (KV9) and Lactobacillus vaginalis FN3 (FN3) on reducing allergic reactions and regulating immune cell function. The results showed that oral administration of KV9 and FN3 significantly reduced the scores of allergic symptoms, hypothermia symptoms, and serum levels of β-LG-specific immunoglobulins E (β-LG-sIgE), histamine, and mast cell protease in allergic mice. Flow cytometry analysis of intestinal dendritic cells (DCs) showed that the proportion of CD11c+major histocompatibility complex (MHC)-II+DCs, CD11c+CD80+DCs, and CD11c+ CD86+DCs increased after KV9 and FN3 intervention, indicating that the strains induced immature DCs and decreased the antigen-presenting capacity of DCs. Meanwhile, the toll-like receptor 4 (TLR4)-NF-κB signaling pathway was activated in DCs. The secretion of interleukin-12 (IL-12) was significantly increased, while interleukin-4 (IL-4) was decreased by DCs after KV9 and FN3 intervention, indicating that DCs have the potential to promote T-cell differentiation into T helper type 1 (Th1) cells. Furthermore, the proportion of CD3+CD8−IFN-γ+ T cells in the spleen increased, while CD3+CD8−IL-4+T cells decreased after oral administration of KV9 and FN3, correcting the T helper type 2 (Th2)-skewed immune responses. These results indicate that KV9 and FN3 reduce β-LG-induced allergic symptoms in mice, and suggest that the two potential probiotics might be used as an alternative therapeutic agent for mitigating food allergy.

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Oral anaphylaxis to peanut in a mouse model is associated with gut permeability but not with Tlr4 or Dock8 mutations

Cited by 20 publications

References 43 publications

Risk factors for severe reactions in food allergy: Rapid evidence review with meta‐analysis

Risk factors for severe reactions in food allergy: Rapid evidence review with meta‐analysis

Reply

Bifidobacterium animalis KV9 and Lactobacillus vaginalis FN3 alleviated β-lactoglobulin-induced allergy by modulating dendritic cells in mice

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