Oral administration of tea saponins to relive oxidative stress and immune suppression in chickens

Abstract

The present study was designed to evaluate the effects of tea saponins on oxidative stress induced by cyclophosphamide in chickens. One hundred twenty chickens were randomly divided into 5 groups. Groups 3 to 4 received intramuscular injection of cyclophosphamide to induce oxidative stress and immunosuppression. After that, groups 2 and 4 were orally administered tea saponins in drinking water for 7 d. Then, groups 1 to 4 were immunized with a live, bivalent vaccine of Newcastle disease virus and infectious bronchitis virus. Blood samples were collected for analysis of oxidative parameters and specific antibody titers, and splenocytes were prepared for lymphocyte proliferative assay. The results showed that administration of tea saponins significantly increased total antioxidant capacity, total superoxide dismutase, catalase, glutathione peroxidase, glutathione, ascorbic acid, and α-tocopherol, and decreased malondialdehyde and protein carbonyl. Enhanced immune responses, such as lymphocyte proliferation induced by concanavalin A and lipopolysaccharides, and serum Newcastle disease virus- and infectious bronchitis virus-specific antibodies were also observed in chickens injected with or without cyclophosphamide. In addition, no side effects were found in chickens throughout the study. Therefore, tea saponins may be a potential agent to improve imunosuppression induced by oxidative stress in chickens.

Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide

“…As a result, LJP-M and LJP-H markedly ameliorated the splenic lymphocyte proliferation of immunosuppressive mice induced by CTX, indicating that LJP is conducive to the activation of lymphocyte proliferation. These results are in accordance with previous studies [ 28 – 30 ].…”

Study on immunoregulation function of peony seed proteolysis product in mice

“…Lymphocyte proliferation assay is a commonly used method to detect cellular immune function (Chinthakindi et al., 2013; Walsh et al., 2015). The lymphocyte transformation rate post‐stimulation using ConA is frequently used to evaluate the T‐cell immune response (Chen et al., 2015; Chi et al., 2017). To investigate the regulatory effect of peony seed proteolysis product on cellular immune function in mice, the proliferation of the spleen lymphocytes at three time points following gavage was assessed, and the results are shown in Figure 1.…”

Antiviral activity of a novel mixture of natural antimicrobials, in vitro, and in a chicken infection model in vivo

“…A similar effect was observed in infections caused by influenza A virus where inclusion of MnSOD in aerosols led to a mild improvement of the disease. Other natural antimicrobials are also known for their antioxidant effect 12 also when included in the feed of farmed poultr 34 with a direct effect in controlling the levels of MnSOD 35 .…”