Oral administration of levan polysaccharide reduces the alloxan-induced oxidative stress in rats

Dahech, Imen; Belghith, Karima; Hamden, Khaled; Feki, Abdelfattah El; Belghith, Hafedh; Mejdoub, Hafedh

doi:10.1016/j.ijbiomac.2011.08.011

Cited by 56 publications

(34 citation statements)

References 37 publications

(29 reference statements)

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“…Oat bran-derived non-starch polysaccharides acted as an active ingredient decreasing the postprandial hyperglycemic response in humans with type 2 diabetes (39). The levan polysaccharide also was efficient in inhibiting hyperglycemia and oxidative stress induced by diabetes (40). A similar result was observed in humans after eating β-glucan-enriched breads (41).…”

Section: Discussionsupporting

confidence: 72%

Polysaccharide isolated from Triticum aestivum stimulates insulin release from pancreatic cells via the ATP-sensitive K+ channel

et al. 2012

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Abstract. Traditional natural plants have been used throughout the world for their antidiabetic effects. The aim of the present study was to investigate the stimulating activity of a polysaccharide extract derived from T. aestivum sprout (TASP) on insulin secretion in vitro using the RIN-5F pancreatic β-cell line and rat pancreatic islets. In these experiments, TASP (0.1 to 2 mg/ml) augmented glucose-stimulated insulin secretion in a dose-dependent manner in the presence of a stimulatory glucose concentration (16.7 mM), but not of a basal concentration (1.1 mM). Although TASP failed to enhance the high K + -induced insulin secretion, the insulinotropic effect of TASP was significantly inhibited by diazoxide, an opener of ATP-sensitive K + channel blocking insulin release. TASP potentiated the insulin secretion induced by other secretagogues, such as IBMX and tolbutamide. Moreover, glucose-derived blood insulin levels were significantly elevated by oral administration of TASP to mice, similarly to antidiabetic drugs. We also demonstrated that TASP significantly increased glucose-induced 45 Ca2+ uptake and proinsulin mRNA expression in rat islets. Overall, our results suggest that TASP has a stimulating effect on insulin secretion and production in pancreatic β-cells via K + channel closure and calcium influx. These results suggest that TASP may be useful as a candidate for the therapy of diabetes mellitus.

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Section: Discussionsupporting

confidence: 72%

Polysaccharide isolated from Triticum aestivum stimulates insulin release from pancreatic cells via the ATP-sensitive K+ channel

et al. 2012

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“…Furthermore, the cytotoxic action of alloxan is mediated mainly by the generation of reactive oxygen species (ROS) (Dahech, Belghith et al 2011). ROS produce fragmentation and damage of DNA of β -cells exposed to alloxan (Ebelt, Peschke et al 2000).…”

Section: Discussion:-mentioning

confidence: 99%

Cytoprotective Effect of Costus Speciosus on Pancreatic Β-Cells in Alloxan-Treated Rats.

Al-brakati¹,

Belal²,

Alsbery³

2017

IJAR

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“…These reactive oxygen species with a simultaneous massive increase in cytosolic calcium concentration causes rapid destruction of Β-cells [37]. Decreasing the islets cell numbers, cell damage, and cell death [38], thickened and hyalinized blood vessels causing hypoxia and resulting in degenerative changes and necrosis [39] and structural and functional alterations such as disorganization of pancreatic architecture and depletion of insulin producing cells [40][41][42] are the significant pancreatic histological changes observed in alloxan induction. Histopathology reports of CF-17 and CF-20 of L. micranthus have shown clear confirmative results of regeneration of pancreatic connective tissue, acini, blood vessels, blood capillaries, pancreatic ducts and their walls, islets capsule, islets cells in accordance with their organization, number, texture and dispersion in the pancreatic lobes.…”

Section: Discussionmentioning

confidence: 99%

Gc-Ms Study of Two Column Fractions from Methanol Extracts of Loranthus Micranthus and Their In Vivo Antidiabetic Activity on Alloxan Induced Diabetic Rats

M¹

2015

J Diabetes Metab

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The methanol extracts of Loranthus micranthus fractions were collected from column chromatography. From TLC, the fraction 17 and 20 were selected based on the clear distiguishable of bands and few phytochemicals. Same fractions when subjected to GC-MS, fraction-17 yielded 13 and fraction-20 yielded 11 prominent compounds. Diabetic rats that were treated with the fraction 17 and 20 showed significantly increase in body weight (16.52 and 12.43 % respectively). Hypoglycemic effect and antihyperlipidemic characters (decrease in the previously elevated levels of serum cholesterol and serum triglycerides) were observed. The two fractions treated rats have also showed the regeneration of pancreatic connective tissue, acini, blood vessels, blood capillaries, pancreatic ducts and their walls, islets capsule and cells in accordance to proper cytoarchitecture. The in vivo antidiabetic property may be due to the presence of single or all phytochemicals in the fraction. Greater activity was observed in fraction-17. However, further experimental studies are still needed for identification of the functionally relevant phytochemical(s) in management of diabetes in rats.sp. were the common host plants of L. micranthus [10]. Herbals are the choice over synthetic drugs from past centuries having multiple therapeutic functions and fewer side effects. In this context it was an effort to attend towards antidibetic studies of L. micranthus in an analytical and systematic ways. L. micranthus has been widely used in ethnomedicine for various purposes, including antihypertensive, anticancer, antispasmodic, and antidiabetic, in treatment of epilepsy, headache, infertility, menopausal syndrome and rheumatism [11,12] and many of medicinal activities are proven by recent researches. It decreases the blood glucose level and controls the loss of body weight in diabetes mellitus [13].Presently the study was aimed to carry out GS-MS and in vivo antidiabetic activities of Loranthus micranthus on alloxan induced diabetic rats. Based on our earlier studies of qualitative, quantitative and some biological activities, column fractions from methanol extracts of L. micranthus were selected for in vivo antidiabetic studies on rats. Body weight, blood glucose level, lipid profile and histopathological changes of rats were considered in the study. Materials and Methods Collection and processing of plantThe fresh leaves of Loranthus micranthus growing on the host plant

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Oral administration of levan polysaccharide reduces the alloxan-induced oxidative stress in rats

Cited by 56 publications

References 37 publications

Polysaccharide isolated from Triticum aestivum stimulates insulin release from pancreatic cells via the ATP-sensitive K+ channel

Polysaccharide isolated from Triticum aestivum stimulates insulin release from pancreatic cells via the ATP-sensitive K+ channel

Cytoprotective Effect of Costus Speciosus on Pancreatic Β-Cells in Alloxan-Treated Rats.

Gc-Ms Study of Two Column Fractions from Methanol Extracts of Loranthus Micranthus and Their In Vivo Antidiabetic Activity on Alloxan Induced Diabetic Rats

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