Oral administration of Bifidobacterium bifidum TMC3115 to neonatal mice may alleviate IgE-mediated allergic risk in adulthood

Abstract

This study aimed to demonstrate whether exposure to bifidobacteria during early life influences immunity and alleviates the risk of immunoglobulin E (IgE)-mediated allergies in adulthood. BALB/c neonatal mice (n=54) were administered with a lyophilised cell preparation of Bifidobacterium bifidum TMC3115 (TMC3115) for 3 weeks. Following the intervention, the mice were immunised with intraperitoneal ovalbumin (OVA). The morphology and function of the intestinal epithelium were determined using histopathological examinations. Intestinal microbiota was detected using quantitative PCR and characterised using next-generation sequencing of 16S rRNA genes from faecal DNA. Caecal short-chain fatty acids (SCFAs) were measured using gas chromatography-mass spectrometry. Serum levels of tumour necrosis factor (TNF)-α, interleukin (IL)-6, IL-10, and immunoglobulin E (IgE) and the percentage of splenic CD4+ T cells were examined using enzyme-linked immunosorbent assay and flow cytometry, respectively. TMC3115 did not significantly affect body weight, and cause any severe systemic inflammation or other clinical symptoms among the neonatal or adult mice, although the crypt depths and Muc2-positive cells in some intestinal segments of neonatal mice were significantly lower than control. Oral TMC3115 administration significantly increased faecal microbial diversity, relative abundance of Bacteroidetes and caecal SCFAs production in neonatal mice. Following the intervention, neonatal mice treated with TMC3115 exhibited less increase in serum IgE levels induced by OVA in adults and significantly higher TNF-α and IL-10 levels than in control. Our findings indicate that the oral administration of bifidobacteria, particularly certain strains, such as TMC3115, during early life could alleviate the risk of IgE-mediated allergies in adult host animals. Modifications of intestinal microbiota, SCFAs metabolism and anti-inflammatory cytokine IL-10 production by bifidobacteria may at least in part be a key mechanism underlying the effect of bifidobacteria on the IgE-mediated immune sensitivity of hosts to attacks by allergens at both neonatal and adult stages.

Bifidobacterium bifidumTMC3115 ameliorates milk protein allergy in by affecting gut microbiota: A randomized double‐blind control trial

“…The results were consistent with the previous report Bifidobacterium species inhibits IgE production in the immune modulation (Takahashi et al., 2006). Recent work also demonstrated that oral administration of B. bifidum TMC3115 may alleviate IgE‐mediated allergic risk in an animal model (Cheng et al., 2018). On the contrary, IgG2 as IgG subclass may monitor allergen immunological activity (Movérare et al, 2017).…”

“…Comparing with the present work, the effects of B. bifidum TMC3115 on allergy were mostly limited to animal study. Oral administration of B. bifidum TMC3115 in neonatal mice was found to attenuate IgE‐mediated allergic risk in adulthood (RY Cheng et al., 2018). B. bifidum TMC3115 consumption in neonatal mice affected gut microbiota and immunity in adulthood (Cheng et al., 2019).…”

“…B. bifidum TMC3115 treatment exerted beneficial effects on the serum cholesterol metabolism in the dyslipidemia patients by altering intestinal microbiota (Ke Wang et al., 2019). Oral administration of the strains TMC3115 could alleviate IgE‐mediated allergies in adult host animals by regulating gut microbiota, SCFAs metabolism and anti‐inflammatory cytokine IL‐10 (Cheng et al., 2018). TMC3115 modulates intestinal microbiota and SCFAs metabolism, and affects gut microbiota and immunity.…”

A high-fat diet and high-fat and high-cholesterol diet may affect glucose and lipid metabolism differentially through gut microbiota in mice

“…Fecal microbiota communities were determined by 16S rRNA sequencing using universal primers (forward primer, 338F, 5′-ACTCCTACGGGAGGCAGCAG-3′; reverse primer, 806R, 5′-GGACTACHVGGGTWTCTAAT-3′) [8]. PCR amplification was performed in a 25 μl reaction mixture containing 50 ng of template DNA, 12.5 μl of Phusion Hot Start Flex 2× Master Mix (New England Biolabs Inc., Beverly, MA, USA), and 2.5 μl of each primer.…”

“…The bioinformatics analysis was performed as described previously [8]. Briefly, the sequencing data were filtered, and the effective tags were clustered into operational taxonomic units (OTUs) with a similarity of 97% using the Uparse 7.0.1001 software (https://drive5.com/uparse/).…”