Oral administration of a streptococcal antigen coupled to cholera toxin B subunit evokes strong antibody responses in salivary glands and extramucosal tissues

Abstract

Generation of local and systemic immune responses by the oral administration of antigens is frequently inefficient, requiring large quantities of immunogens and yielding only modest antibody responses. In this study, we have demonstrated that oral administration of microgram amounts of Streptococcus mutans protein antigen I/TI covalently coupled to the B subunit of cholera toxin elicits vigorous mucosal as well as extramucosal immunoglobulin A and G antistreptococcal antibody responses in mice. These responses were manifested by the presence of large numbers of antibody-secreting cells in salivary glands, mesenteric lymph nodes, and spleens and by the development of high levels of circulating antibodies. This novel immunization strategy may find broad application in the construction of oral vaccines for the control of infectious diseases caused by pathogens encountered at mucosal and extramucosal sites.

Oral tolerization with peptide 336–351 linked to cholera toxin B subunit in preventing relapses of uveitis in Behcet's disease

“…This peptide was coupled to a highly purified, recombinant CTB (rCTB) produced under GMP conditions by SBL Vaccine (Stockholm, Sweden), commonly used as a component of the internationally registered oral cholera vaccine (Dukoral). We used the bifunctional cross-linking reagent N-succinimidyl 3-(2-pyridyldithio)proportionate (SPDP) (Pierce Rockford, IL, USA), with a substitution ratio of 4-5 residues per rCTB [27]. The samples were purified by Sephadex G25 Chromatography in PBS (Amersham Biosciences, Uppsala, Sweden).…”

Mucosal immunity and tolerance: relevance to vaccine development

“…Mucosal iectin-like molecules as carrier-delivery systems Oral administration of small amounts of protein antigens covalently coupled to carrier moiecuies with known affinity for mucosal epithelial cells, such as CT, Escherichia coii heat-labile toxin (LT), CT-B and LT-B, can eUcit mucosal and also, under certain conditions, systemic antibody responses to the conju-gated antigen (136)(137)(138). This strategy has also recently been The ability of a vector to co!onize specific mucosa!…”

Immune responses to common respiratory pathogens: problems and perspectives in equine immunology

“…High levels of antibody to CPS correlate with protection in other species (Gillespie 1989). but there is no evidence that respiratory tract antibody protects from infection with S. pneurnoniae, although mucosal immunity is important in protection from S. equi (above) and other streptococcal infections (Czerkisky et a/ 1989). Therefore, it appears that the generation and duration of antibody responses to CPS offer the best first approach to targeting effective vaccines for S .…”