Orai channel-mediated Ca²⁺signals in vascular and airway smooth muscle

Abstract

Spinelli AM, Trebak M. Orai channel-mediated Ca 2ϩ signals in vascular and airway smooth muscle.

Lipids at membrane contact sites: cell signaling and ion transport

“…The activation of the ARC channel, which is composed of a STIM1-assembled Orai1/ Orai3 heteromer, was reported to occur in response to AA [123]. Subsequent studies indicated that the physiological activation of the ARC channels is mediated by LCT4 rather than AA (discussed in [130]). Since STIM1 activates the Orai channels at the ER/PM junctions, also ARC channel activation might occur at the ER/PM junction [131].…”

Evaluation of known and novel inhibitors of Orai1-mediated store operated Ca 2+ entry in MDA-MB-231 breast cancer cells using a Fluorescence Imaging Plate Reader assay

“…[5][6][7] Orai1 is implicated in a variety of physiological functions including processes important in immunity, 5 lactation 8 and the cardiovascular and respiratory systems. 9 A number of studies have also linked Orai1 with cancer. 10,11 For example, basal molecular subtype breast cancers associated with poor prognosis express elevated levels of Orai1 and alterations in the relative levels of the Orai1 activators STIM1 and STIM2.…”

Protease‐activated receptor 2 activates CRAC‐mediated Ca 2+ influx to cause prostate smooth muscle contraction

“…Taken together, these studies demonstrate that STIM/Orai coupling contributes to the phenotypic remodeling of vascular and pulmonary smooth muscles in response to injury. Therefore, it was argued that SOC‐mediated intracellular Ca 2+ increase is not necessary for smooth muscle contraction . However, this notion has been challenged in light of new evidence that showed contraction of airway smooth muscle cells in precision cut lung slices can be blocked by inhibiting STIM/Orai coupling .…”

“…Therefore, it was argued that SOC-mediated intracellular Ca 2+ increase is not necessary for smooth muscle contraction. 24,28 However, this notion has been challenged in light of new evidence that showed contraction of airway smooth muscle cells in precision cut lung slices can be blocked by inhibiting STIM/Orai coupling. 29 In contrast, blocking L-type channels had reduced effect on contraction of these cells.…”