OR2H2 regulates the differentiation of human myoblast cells by its ligand aldehyde 13-13

Kalbe, Benjamin; Osterloh, Markus; Schulz, Viola M.; Altmüller, Janine; Becker, Christian; Osterloh, Sabrina; Hatt, Hanns

doi:10.1016/j.abb.2018.03.017

Cited by 12 publications

(22 citation statements)

References 45 publications

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“…Interestingly, despite OLFRs have been shown to facilitate perception of smell in the brain through sensing volatile chemicals (i.e. odorants) in the nasal olfactory epithelium, ectopic expression of OLFRs occurs also in non-chemosensory tissues like skeletal muscle [46], where these receptors are also functional and play a role in skeletal muscle development and regeneration [47,48]. It is thus possible that IM modulates processes involved in skeletal muscle development and regeneration via regulating the expression of OLFRs.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive evaluation of the metabolic effects of insect meal from Tenebrio molitor L. in growing pigs by transcriptomics, metabolomics and lipidomics

Meyer

Geßner

Braune

et al. 2020

J Animal Sci Biotechnol

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Background: The hypothesis was tested that insect meal (IM) as protein source influences intermediary metabolism of growing pigs. To test this, 30 male, 5-week-old crossbred pigs were randomly assigned to 3 groups of 10 pigs each with similar body weights (BW) and fed isonitrogenous diets either without (CON) or with 5% IM (IM5) or 10% IM (IM10) from Tenebrio molitor L. for 4 weeks and key metabolic tissues (liver, muscle, plasma) were analyzed using omics-techniques. Results: Most performance parameters did not differ across the groups, whereas ileal digestibilities of most amino acids were 6.7 to 15.6%-units lower in IM10 than in CON (P < 0.05). Transcriptomics of liver and skeletal muscle revealed a total of 166 and 198, respectively, transcripts differentially expressed between IM10 and CON (P < 0.05). Plasma metabolomics revealed higher concentrations of alanine, citrulline, glutamate, proline, serine, tyrosine and valine and a lower concentration of asparagine in IM10 than in CON (P < 0.05). Only one out of fourteen quantifiable amino acid metabolites, namely methionine sulfoxide (MetS), in plasma was elevated by 45% and 71% in IM5 and IM10, respectively, compared to CON (P < 0.05). Plasma concentrations of both, major carnitine/ acylcarnitine species and bile acids were not different across groups. Lipidomics of liver and plasma demonstrated no differences in the concentrations of triacylglycerols, cholesterol and the main phospholipids, lysophospholipids and sphingolipids between groups. The percentages of all individual phosphatidylcholine (PC) and phosphatidylethanolamine (PE) species in the liver showed no differences between groups, except those with 6 double bonds (PC 38:6, PC 40:6, PE 38:6, PE 40:6), which were markedly lower in IM10 than in CON (P < 0.05). In line with this, the percentage of C22:6n-3 in hepatic total lipids was lower in IM10 than in the other groups (P < 0.05). Conclusions: Comprehensive analyzes of the transcriptome, lipidome and metabolome of key metabolic tissues indicate that partial or complete replacement of a conventional protein source by IM in the diet has only a weak impact on the intermediary metabolism of growing pigs. Thus, it is concluded that IM from Tenebrio molitor L. can be used as a dietary source of protein in pigs without causing adverse effects on metabolism.

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Section: Discussionmentioning

confidence: 99%

Comprehensive evaluation of the metabolic effects of insect meal from Tenebrio molitor L. in growing pigs by transcriptomics, metabolomics and lipidomics

Meyer

Geßner

Braune

et al. 2020

J Animal Sci Biotechnol

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“…To identify OR expression in the human thyroid gland, we first analyzed next-generation sequencing (NGS) data previously published by Flegel et al (2013). The two receptors OR2H2 and OR2W3 were selected for further experiments according to their expression level and because activating ligands for both ORs are known (Flegel et al, 2015;Kalbe et al, 2018). To verify the ORs' expression, human thyroid tissue samples of papillary (PTC) and follicular thyroid cancer (FTC), as well as the healthy surrounding tissue, were analyzed by RT-PCR.…”

Section: Olfactory Receptors 2h2 and 2w3 Are Expressed In Benign And Malignant Human Thyroid Tissuementioning

confidence: 99%

“…Therefore, we used the Ca 2+ imaging technique to analyze whether OR2H2 and OR2W3 were functionally expressed in the thyroid cell lines. For this purpose, the already known ligands aldehyde 13-13 for OR2H2 and nerol for OR2W3 were applied to the cells at a concentration of 500 μM (Figure 3; Supplementary Figure S1; Flegel et al, 2015;Kalbe et al, 2018). In the following Ca 2+ imaging experiments, we focused on the result of aldehyde 13-13 induced signal in Nthy-ori 3-1 cells in more detail, since these were more suitable due to a more stable reaction, and thus, an investigation of all desired inhibitors was possible.…”

Section: Or2h2 and Or2w3 Expression In Human Thyroid Cell Linesmentioning

confidence: 99%

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Functional Characterization of Olfactory Receptors in the Thyroid Gland

et al. 2021

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Olfactory receptors (ORs) are almost ubiquitously expressed in the human body. However, information about their functions in these tissues is lacking. To date, no functional characterization of expressed ORs in the human thyroid has been performed. In this study, we detected and compared the expression of OR2H2 and OR2W3 in healthy and malignant cell lines and their corresponding tissues, respectively. We demonstrated that stimulation of ORs by their specific ligand resulted in a transient increase in intracellular calcium and cAMP concentrations. In the case of OR2H2, the downstream signaling cascade analysis revealed that adenylate cyclase (AC) and phosphoinositide phospholipase C (PLC) were involved. Furthermore, OR2H2 and OR2W3 activation affected migration, proliferation, and invasion. These are the first insights that ORs influence physiology-relevant processes in the healthy and malignant thyroid.

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“…However, wholegenome sequencing studies convincingly demonstrated that these receptors are also ectopically expressed in non-chemosensory tissues like testis, kidney and skeletal muscle (71) , where these receptors are also functional and activated by endogenous ligands. Interestingly, recent evidence indicates that skeletal muscle Olfr isoforms play a role in skeletal muscle development, regeneration and even muscular dystrophy (72,73) . Thus, although the biological significance of the results from GSEA should also be interpreted cautiously considering the overall very moderate regulation of genes by carnitine supplementation in mice, the regulation of more than sixty genes encoding chemosensory receptors by carnitine supplementation in skeletal muscle of 19-month-old mice strongly suggests that supplemental carnitine has the potential to modulate processes involved in muscle physiology and pathophysiology.…”

Section: Discussionmentioning

confidence: 99%

Effect of lifelong carnitine supplementation on plasma and tissue carnitine status, hepatic lipid metabolism and stress signalling pathways and skeletal muscle transcriptome in mice at advanced age

et al. 2019

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While strong evidence from clinical studies suggests beneficial effects of carnitine supplementation on metabolic health, serious safety concerns associated with carnitine supplementation have been raised from studies in mice. Considering that the carnitine doses in these mice studies were up to 100 times higher than those used in clinical studies, the present study aimed to address possible safety concerns associated with long-term supplementation of a carnitine dose used in clinical trials. Two groups of NMRI mice were fed either a control or a carnitine-supplemented diet (1 g/kg diet) from weaning to 19 months of age, and parameters of hepatic lipid metabolism and stress signalling and skeletal muscle gene expression were analysed in the mice at 19 months of age. Concentrations of free carnitine and acetylcarnitine in plasma and tissues were higher in the carnitine than in the control group (P<0·05). Plasma concentrations of free carnitine and acetylcarnitine were higher in mice at adult age (10 and 15 months) than at advanced age (19 months) (P<0·05). Hepatic mRNA and protein levels of genes involved in lipid metabolism and stress signalling and hepatic and plasma lipid concentrations did not differ between the carnitine and the control group. Skeletal muscle transcriptome analysis in 19-month-old mice revealed only a moderate regulation between carnitine and control group. Lifelong carnitine supplementation prevents an age-dependent impairment of plasma carnitine status, but safety concerns associated with long-term supplementation of carnitine at doses used in clinical trials can be considered as unfounded.

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OR2H2 regulates the differentiation of human myoblast cells by its ligand aldehyde 13-13

Cited by 12 publications

References 45 publications

Comprehensive evaluation of the metabolic effects of insect meal from Tenebrio molitor L. in growing pigs by transcriptomics, metabolomics and lipidomics

Comprehensive evaluation of the metabolic effects of insect meal from Tenebrio molitor L. in growing pigs by transcriptomics, metabolomics and lipidomics

Functional Characterization of Olfactory Receptors in the Thyroid Gland

Effect of lifelong carnitine supplementation on plasma and tissue carnitine status, hepatic lipid metabolism and stress signalling pathways and skeletal muscle transcriptome in mice at advanced age

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