Optrodes for combined optogenetics and electrophysiology in live animals

Dufour, Suzie; Koninck, Yves De

doi:10.1117/1.nph.2.3.031205

Cited by 72 publications

(46 citation statements)

References 129 publications

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“…Optrodes were created as previously described. 18 Single carbon fibers (~8 cm long) coupled to silver wire were epoxied into glass capillary tubes. A micropipette puller (Sutter Instrument Co Model P-97) melted and pulled the capillary tube to a fine point around the fiber.…”

Section: Methodsmentioning

confidence: 99%

Divergent functions of the left and right central amygdala in visceral nociception

Sadler

McQuaid²,

Cox³

et al. 2016

Pain

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The left and right central amygdalae (CeA) are limbic regions involved in somatic and visceral pain processing. These 2 nuclei are asymmetrically involved in somatic pain modulation; pain-like responses on both sides of the body are preferentially driven by the right CeA, and in a reciprocal fashion, nociceptive somatic stimuli on both sides of the body predominantly alter molecular and physiological activities in the right CeA. Unknown, however, is whether this lateralization also exists in visceral pain processing and furthermore what function the left CeA has in modulating nociceptive information. Using urinary bladder distension (UBD) and excitatory optogenetics, a pronociceptive function of the right CeA was demonstrated in mice. Channelrhodopsin-2–mediated activation of the right CeA increased visceromotor responses (VMRs), while activation of the left CeA had no effect. Similarly, UBD-evoked VMRs increased after unilateral infusion of pituitary adenylate cyclase–activating polypeptide in the right CeA. To determine intrinsic left CeA involvement in bladder pain modulation, this region was optogenetically silenced during noxious UBD. Halorhodopsin (NpHR)-mediated inhibition of the left CeA increased VMRs, suggesting an ongoing antinociceptive function for this region. Finally, divergent left and right CeA functions were evaluated during abdominal mechanosensory testing. In naive animals, channelrhodopsin-2–mediated activation of the right CeA induced mechanical allodynia, and after cyclophosphamide-induced bladder sensitization, activation of the left CeA reversed referred bladder pain–like behaviors. Overall, these data provide evidence for functional brain lateralization in the absence of peripheral anatomical asymmetries.

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Section: Methodsmentioning

confidence: 99%

Divergent functions of the left and right central amygdala in visceral nociception

Sadler

McQuaid²,

Cox³

et al. 2016

Pain

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“…This approach shows two major limitations: (i) the size of the implanted waveguide significantly damages the brain tissue, and (ii) it is not possible to redirect light in a different zone of the brain. Several alternative technological solutions have been developed recently to dynamically redirect light towards different locations of the brain tissue [8][9][10][11], including µ-Light Emitting Diodes [6,12,13], array of waveguides [14][15][16][17], bundle of optical fibers [18,19] or patterned illumination techniques [4,20,21].…”

Section: Introductionmentioning

confidence: 99%

Modal demultiplexing properties of tapered and nanostructured optical fibers for in vivo optogenetic control of neural activity

Patria

Sileo

Sabatini

et al. 2015

Biomed. Opt. Express

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Optogenetic approaches to manipulate neural activity have revolutionized the ability of neuroscientists to uncover the functional connectivity underlying brain function. At the same time, the increasing complexity of in vivo optogenetic experiments has increased the demand for new techniques to precisely deliver light into the brain, in particular to illuminate selected portions of the neural tissue. Tapered and nanopatterned gold-coated optical fibers were recently proposed as minimally invasive multipoint light delivery devices, allowing for site-selective optogenetic stimulation in the mammalian brain [Pisanello et al., Neuron 82, 1245]. Here we demonstrate that the working principle behind these devices is based on the mode-selective photonic properties of the fiber taper. Using analytical and ray tracing models we model the finite conductance of the metal coating, and show that single or multiple optical windows located at specific taper sections can outcouple only specific subsets of guided modes injected into the fiber.

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“…The hemoglobin optical absorbance signature (520-620 nm) is noticeable in scalp tissues (unlike the implant) limiting the access in that wavelength range 59 . Figure 3( 5,9,64 are a few examples of optical techniques that could benefit from the improved transmission in offered by the YSZ implant. Complementarily, any potential adhesion of biochemical agents and/or tissue growth on the implant (e.g., fibrotic tissue, proteins, cell adhesion) could be potentially monitored over time 65 .…”

Section: Discussionmentioning

confidence: 99%

Theranostic cranial implant for hyperspectral light delivery and microcirculation imaging without scalp removal

Davoodzadeh¹,

Cano‐Velázquez

Jonak³

et al. 2019

Preprint

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Light based techniques for imaging, diagnosing and treating the brain have become widespread clinical tools, but application of these techniques is limited by optical attenuation in the scalp and skull. This optical attenuation reduces the achievable spatial resolution, precluding the visualization of small features such as brain microvessels. The goal of this study was to assess a strategy for providing ongoing optical access to the brain without the need for repeated craniectomy or retraction of the scalp. This strategy involves the use of a transparent cranial implant and skin optical clearing agents, and was tested in mice to assess improvements in optical access which could be achieved for laser speckle imaging of cerebral microvasculature. Combined transmittance of the optically cleared scalp overlying the transparent cranial implant was as high as 89% in the NIR range, 50% in red range, 24% in green range, and 20% in blue range. In vivo laser speckle imaging experiments of mouse cerebral blood vessels showed that the proposed optical access increased signal-to-noise ratio and image resolution, allowing for visualization of microvessels through the transparent implant, which was not possible through the uncleared scalp and intact skull.

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Optrodes for combined optogenetics and electrophysiology in live animals

Cited by 72 publications

References 129 publications

Divergent functions of the left and right central amygdala in visceral nociception

Divergent functions of the left and right central amygdala in visceral nociception

Modal demultiplexing properties of tapered and nanostructured optical fibers for in vivo optogenetic control of neural activity

Theranostic cranial implant for hyperspectral light delivery and microcirculation imaging without scalp removal

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