Optosensing Determination of Parts-per-Billion of Metals

Lacy, Nathan; Christian, Gary D.; Růžička, Jaromír

doi:10.1006/mchj.1998.1696

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…A stream that contains a substrate passes over the immobilized enzymes and reacts with the coated surface to convert the substrate into a detectable product. For example, the confirmation of metals has been achieved at parts‐per‐billion detection limits with BI‐FIA formats (Lacy, Christian, & Ruzicka, 1999).…”

Section: Controlled Band‐dispersion Methodsmentioning

confidence: 99%

Controlled band dispersion for quantitative binding determination and analysis with electrospray ionization‐mass spectrometry

Schug¹,

Serrano²,

Fryčák³

2009

Mass Spectrometry Reviews

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This review discusses recent emerging techniques that have been used to couple flow-injection analysis (FIA) and electrospray ionization-mass spectrometry (ESI-MS) for the quantitation of noncovalent binding interactions. Focus is placed predominantly on two such methods. Diffusion-based measurements, developed by Konermann and co-workers, uses controlled-band dispersion prior to ESI-MS to determine diffusion constants and binding constants based on the temporal variation of ligand signal measured in the mass spectrum (an indirect technique). Dynamic titration, developed by Schug and co-workers, is a direct method, where a temporal compositional gradient of a guest molecule is induced in the presence of host in solution to monitor the concentration dependence of complex formation as a function of observed complex ion abundance after ESI-MS. Further discussion places these techniques in the context of a variety of other direct and indirect ESI-MS-based binding determination methods, and highlights advantages, disadvantages, and practical considerations for their proper use to investigate a broad range of macromolecular and small-molecule interaction systems.

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Section: Controlled Band‐dispersion Methodsmentioning

confidence: 99%