Optomechanical measurement of the role of lamins in whole cell deformability

Kolb, Thorsten; Kraxner, Julia; Skodzek, Kai; Haug, Michael; Crawford, Dean Frazer; Maass, Kendra K.; Aifantis, Katerina E.; Whyte, Graeme

doi:10.1002/jbio.201600198

Cited by 3 publications

(1 citation statement)

References 55 publications

(63 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increase of laser power induces a cell stretching, revealing the time-dependent mechanical response of the cell. This tool has been recently used by Kolb et al [ 65 ] to probe the nuclear properties of both adult mouse fibroblasts (adherent cell line) and K562 cells (suspension cell line) with different nuclear lamin A levels. Authors demonstrated that, in physiological conditions, the nucleus deforms less than the surrounding cytoskeleton.…”

Section: Mechanical Characterization Techniquesmentioning

confidence: 99%

A Perspective on the Experimental Techniques for Studying Lamins

Pecorari

Borin

Sbaizero

2017

Cells

View full text Add to dashboard Cite

Lamins are type V intermediate filaments that collectively form a meshwork underneath the inner nuclear membrane, called nuclear lamina. Furthermore, they are also present in the nucleoplasm. Lamins are experiencing a growing interest, since a wide range of diseases are induced by mutations in the gene coding for A-type lamins, globally known as laminopathies. Moreover, it has been demonstrated that lamins are involved in other pathological conditions, like cancer. The role of lamins has been studied from several perspectives, exploiting different techniques and procedures. This multidisciplinary approach has contributed to resolving the unique features of lamins and has provided a thorough insight in their role in living organisms. Yet, there are still many unanswered questions, which constantly generate research in the field. The present work is aimed to review some interesting experimental techniques performed so far to study lamins. Scientists can take advantage of this collection for their novel investigations, being aware of the already pursued and consolidated methodologies. Hopefully, advances in these research directions will provide insights to achieve better diagnostic procedures and effective therapeutic options.

show abstract

Section: Mechanical Characterization Techniquesmentioning

confidence: 99%

A Perspective on the Experimental Techniques for Studying Lamins

Pecorari

Borin

Sbaizero

2017

Cells

View full text Add to dashboard Cite

show abstract

Nuclear Lamin Protein C Is Linked to Lineage-Specific, Whole-Cell Mechanical Properties

et al. 2018

View full text Add to dashboard Cite

show abstract

Dual-fiber microfluidic chip for multimodal manipulation of single cells

et al. 2021

View full text Add to dashboard Cite

On-chip single-cell manipulation is imperative in cell biology and it is desirable for a microfluidic chip to have multimodal manipulation capability. Here, we embedded two counter-propagating optical fibers into the microfluidic chip and configured their relative position in space to produce different misalignments. By doing so, we demonstrated multimodal manipulation of single cells, including capture, stretching, translation, orbital revolution, and spin rotation. The rotational manipulation can be in-plane or out-of-plane, providing flexibility and capability to observe the cells from different angles. Based on out-of-plane rotation, we performed a 3D reconstruction of cell morphology and extracted its five geometric parameters as biophysical features. We envision that this type of microfluidic chip configured with dual optical fibers can be helpful in manipulating cells as the upstream process of single-cell analysis.

show abstract

Optomechanical measurement of the role of lamins in whole cell deformability

Cited by 3 publications

References 55 publications

A Perspective on the Experimental Techniques for Studying Lamins

A Perspective on the Experimental Techniques for Studying Lamins

Nuclear Lamin Protein C Is Linked to Lineage-Specific, Whole-Cell Mechanical Properties

Dual-fiber microfluidic chip for multimodal manipulation of single cells

Contact Info

Product

Resources

About