Optogenetic regulation of transcription

Polesskaya, Oksana; Baranova, Ancha; Bui, Sarah; Кондратьев, Н. В.; Kananykhina, Evgeniya; Nazarenko, Olga; Shapiro, Tatyana; Nardia, Frances Barg; Корниенко, Владимир Юрьевич; Chandhoke, Vikas; Stadler, István; Lanzafame, Raymond J.; Myakishev-Rempel, Max

doi:10.1186/s12868-018-0411-6

Cited by 34 publications

(30 citation statements)

References 68 publications

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“…Historically, chemically regulatable gene expression systems, such as the tetracycline-regulated transcription system [40], has been the most widely used approach to manipulate the expression of gene of interest. Recently, the use of plant flavoproteins (light stimulation) have been engineered to control mammalian transcription factor activity [41]. To date, optogenetic technology has been primarily utilized to alter membrane excitability in neurons using microbial opsins that gate ion channels [42].…”

Section: Introductionmentioning

confidence: 99%

“…To date, optogenetic technology has been primarily utilized to alter membrane excitability in neurons using microbial opsins that gate ion channels [42]. However, an underutilized application of this technology is that of reversible optical regulation of transgene expression [41]. A previous study has utilized a regulatable version of EL222, a bacterial Light-Oxygen-Voltage (LOV) protein that has been shown to bind to DNA when activated with the blue-light [43][44][45][46][47].…”

Section: Introductionmentioning

confidence: 99%

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Optical induction of autophagy via Transcription factor EB (TFEB) reduces pathological tau in neurons

et al. 2020

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Pathological accumulation of microtubule associated protein tau in neurons is a major neuropathological hallmark of Alzheimer's disease (AD) and related tauopathies. Several attempts have been made to promote clearance of pathological tau (p-Tau) from neurons. Transcription factor EB (TFEB) has shown to clear p-Tau from neurons via autophagy. However, sustained TFEB activation and autophagy can create burden on cellular bioenergetics and can be deleterious. Here, we modified previously described two-plasmid systems of Light Activated Protein (LAP) from bacterial transcription factor-EL222 and Light Responsive Element (LRE) to encode TFEB. Upon blue-light (465 nm) illumination, the conformation changes in LAP induced LRE-driven expression of TFEB, its nuclear entry, TFEBmediated expression of autophagy-lysosomal genes and clearance of p-Tau from neuronal cells and AD patient-derived human iPSC-neurons. Turning the blue-light off reversed the expression of TFEB-target genes and attenuated p-Tau clearance. Together, these results suggest that optically regulated TFEB expression unlocks the potential of opto-therapeutics to treat AD and other dementias.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optical induction of autophagy via Transcription factor EB (TFEB) reduces pathological tau in neurons

et al. 2020

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“…Moreover, combining optogenetic inputs with chemical inputs enable further tunability and enable tighter control of genetic circuits [144]. For a recent review of the systems used in the optogenetic regulation of transcription, please refer to [145]. Here we discuss various strategies which can be adopted for achieving transcriptional control.…”

Section: F Control Gene Transcription and Translationmentioning

confidence: 99%

Chemical physics in living cells — Using light to visualize and control intracellular signal transduction

Krishnamurthy

Zhang

2018

Chinese Journal of Chemical Physics

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Cells are crowded microenvironments filled with macromolecules undergoing constant physical and chemical interactions. The physicochemical makeup of the cells affects various cellular responses, determines cell-cell interactions and influences cell decisions. Chemical and physical properties differ between cells and within cells. Moreover, these properties are subject to dynamic changes in response to environmental signals, which often demand adjustments in the chemical or physical states of intracellular molecules. Indeed, cellular responses such as gene expression rely on the faithful relay of information from the outside to the inside of the cell, a process termed signal transduction. The signal often traverses a complex path across subcellular spaces with variable physical chemistry, sometimes even influencing it. Understanding the molecular states of such signaling molecules and their intracellular environments is vital to our understanding of the cell. Exploring such intricate spaces is possible today largely because of experimental and theoretical tools. Here, we focus on one tool that is commonly used in chemical physics studies-light. We summarize recent work which uses light to both visualize the cellular environment and also control intracellular processes along the axis of signal transduction. We highlight recent accomplishments in optical microscopy and optogenetics, an emerging experimental strategy which utilizes light to control the molecular processes in live cells. We believe that optogenetics lends unprecedented spatiotemporal precision to the manipulation of physicochemical properties in biological contexts. We hope to use this work to demonstrate new opportunities for chemical physicists who are interested in pursuing biological and biomedical questions.

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“…Irradiation with light of an appropriate wavelength causes conformational changes, commonly resulting in dimerization or oligomerization of the proteins. Careful engineering of photosensitive domains into enzymes, transcription factors or other proteins of interest endowed light-mediated control over the conformation of these proteins and resulted in light-activatable genetic tools for a variety of applications such as control over gene expression, genome editing, and protein relocalization (Beyer et al, 2015;Buckley et al, 2016;Polesskaya et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Non-neuromodulatory Optogenetic Tools in Zebrafish

Varady

Distel

2020

Front. Cell Dev. Biol.

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The zebrafish (Danio rerio) is a popular vertebrate model organism to investigate molecular mechanisms driving development and disease. Due to its transparency at embryonic and larval stages, investigations in the living organism are possible with subcellular resolution using intravital microscopy. The beneficial optical characteristics of zebrafish not only allow for passive observation, but also active manipulation of proteins and cells by light using optogenetic tools. Initially, photosensitive ion channels have been applied for neurobiological studies in zebrafish to dissect complex behaviors on a cellular level. More recently, exciting non-neural optogenetic tools have been established to control gene expression or protein localization and activity, allowing for unprecedented non-invasive and precise manipulation of various aspects of cellular physiology. Zebrafish will likely be a vertebrate model organism at the forefront of in vivo application of non-neural optogenetic tools and pioneering work has already been performed. In this review, we provide an overview of non-neuromodulatory optogenetic tools successfully applied in zebrafish to control gene expression, protein localization, cell signaling, migration and cell ablation.

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Optogenetic regulation of transcription

Cited by 34 publications

References 68 publications

Optical induction of autophagy via Transcription factor EB (TFEB) reduces pathological tau in neurons

Optical induction of autophagy via Transcription factor EB (TFEB) reduces pathological tau in neurons

Chemical physics in living cells — Using light to visualize and control intracellular signal transduction

Non-neuromodulatory Optogenetic Tools in Zebrafish

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