Optogenetic Modulation of a Minor Fraction of Parvalbumin-Positive Interneurons Specifically Affects Spatiotemporal Dynamics of Spontaneous and Sensory-Evoked Activity in Mouse Somatosensory Cortex in Vivo

Yang, Jenq‐Wei; Prouvot, Pierre-Hugues; Reyes‐Puerta, Vicente; Stüttgen, Maik C.; Stroh, Albrecht; Luhmann, Heiko J.

doi:10.1093/cercor/bhx261

Cited by 35 publications

(44 citation statements)

References 64 publications

(111 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We found strong and dense co-expression across targeted cortical layers II/III and V/VI. Note, that layer IV seems to be difficult to target with AAVs of serotype I/II, as reported previously [28,29].…”

Section: Careful Titration Of Viral Vectors Is Needed To Achieve Strosupporting

confidence: 65%

Navigating the translational roadblock: Towards highly specific and effective all-optical interrogations of neural circuits

Arnoux

Doering

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

contributing first authors One Sentence Summary: We maximize translational relevance of 2-P all-optical physiology by increasing specificity, minimizing artifacts and optimizing stimulation efficacy. Abstract:Two-photon (2-P) all-optical approaches combine in vivo 2-P calcium imaging and 2-P optogenetic modulations and have the potential to build a framework for network-based therapies, e.g. for rebalancing maladaptive activity patterns in preclinical models of neurological disorders. Here, our goal was to tailor these approaches for this purpose: Firstly, we combined in vivo juxtacellular recordings and GCaMP6f-based 2-P calcium imaging in layer II/III of mouse visual cortex to tune our detection algorithm towards a 100 % specific identification of APrelated calcium transients. False-positive-free detection was achieved at a sensitivity of approximately 73 %. To further increase specificity, secondly, we minimized photostimulation artifacts as a potential source for false-positives by using extended-wavelength-spectrum laser sources for optogenetic stimulation of the excitatory opsin C1V1. We achieved artifact-free all-optical experiments performing photostimulations at 1100 nm or higher and simultaneous calcium imaging at 920 nm in mouse visual cortex in vivo. Thirdly, we determined the spectral range for maximizing efficacy of optogenetic control by performing 2-P photostimulations of individual neurons with wavelengths up to 1300 nm. The rate of evoked transients in GCaMP6f/C1V1-coexpressing cortical neurons peaked already at 1100 nm. By refining spike detection and defining 1100 nm as the optimal wavelength for artifact-free and effective stimulations of C1V1 in GCaMP-based all-optical interrogations, we increased the translational value of these approaches, e.g. for the use in preclinical applications of network-based therapies.

show abstract

Section: Careful Titration Of Viral Vectors Is Needed To Achieve Strosupporting

confidence: 65%

Navigating the translational roadblock: Towards highly specific and effective all-optical interrogations of neural circuits

Arnoux

Doering

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Our hypothesis that recurrent and lateral inhibition might be mediated by PV+ interneurons is supported by a recent study showing that optogenetic inhibition of PV+ neurons causes an increase in lateral excitation, whereas optogenetic activation causes a strong reduction (Yang et al . ). In addition, activation of PV+ interneurons is most efficient within a 20 ms window to reduce the responses of the principal barrel.…”

Section: Discussionmentioning

confidence: 97%

Repetitive transcranial magnetic stimulation recovers cortical map plasticity induced by sensory deprivation due to deafferentiation

Kloosterboer

Funke

2019

The Journal of Physiology

View full text Add to dashboard Cite

Key pointsr Partial sensory deprivation (deafferentation) by removing whiskers from the rat snout resulted in a reduced responsiveness of related cortical representations.r Repetitive transcranial magnetic stimulation (three blocks of intermittent theta-burst) applied for 5 days in combination with sensory exploration restored the normal responsiveness level of the deafferented barrel cortex. r However, intracortical inhibition (lateral and recurrent) appeared to be reduced after repetitive transcranial magnetic stimulation, probably as the cause of improved responsiveness.r Repetitive transcranial magnetic stimulation also reduced the asymmetry of the lateral spread of sensory activity.Abstract Repetitive transcranial magnetic stimulation (rTMS) modulates human cortical excitability. It has the potential to support recovery to normal cortical function when the excitation-inhibition balance is altered (e.g. after a stroke or loss of sensory input). We tested cortical map plasticity on the basis of sensory responses (local field potentials, LFPs) and expression of neuronal activity marker proteins within the barrel cortex of rats receiving either active or sham rTMS after selective unilateral deafferentation by whiskers plucking. Rats received daily rTMS [intermittent theta-burst (iTBS), active or sham] for 5 days before exploring an enriched environment. Our previous studies indicated a disinhibitory effect of iTBS on cortical activity. Therefore, we also expected disinhibitory effects if deafferentation causes depression of sensory responses. Deafferentation resulted in an acute general reduction of sensory responsiveness and enhanced expression of inhibitory activity markers (GAD67, parvalbumin) in the deafferented hemisphere. Active but not sham-iTBS-rTMS normalized these measures. The stronger caudal-to-frontal horizontal spread of activity across barrels was reduced after deafferentation but not restored after active iTBS, despite generally increased responses. Fitting the Ellen Kloosterboer studied Biochemistry at the Ruhr-University Bochum, where she completed with a Master's degree in 2011. In 2012, she joined the research team 'Experimental Cortical Plasticity' of Klaus Funke (Department of Neurophysiology, Medical Faculty, RUB), where she investigated the influence of transcranial magnetic stimulation on cortical plasticity in the rat and received her doctorate in 2016. Klaus Funke studied Biology at the Ruhr-University in Bochum, receiving a diploma (1983) and completing doctoral thesis work (1988) on the central somatosensory system of pigeons. As a postdoctoal student, he joined the group of Ulf Eysel (Department of Neurophysiology, Medical School, Ruhr-University Bochum) studying the state-dependent modulation of retinogeniculate signal transmission (habilitation in physiology 1996). Subsequently, he has built up a research team working on animal models of cortical plasticity and, currently, on the cellular mechanisms of transcranial magnetic stimulation. This is an open access article under the ter...

show abstract

“…For example, Zhu et al 13 showed that suppressing PV-neurons is sufficient to increase responses to visual stimuli and improves SNR. Manipulating PV neuron activity also alters the spontaneous activity level in the excitatory neurons 13,14,24,[53][54][55][56][57][58][59][60][61][62] . Our recent study showed that deficit in gap detection associated with noise exposure-induced tinnitus is mostly mediated by reduction in PV-but not SOM-neurons, and activation of PV-neurons using DREADD can partially ameliorate this deficit 63 .…”

Section: Enhanced Inhibition On Evoked and Spontaneous Activities M-mentioning

confidence: 99%

Increasing endogenous activity of NMDARs on GABAergic neurons increases inhibition, alters sensory processing and prevents noise-induced tinnitus

Deng

Masri

Yao

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Selective enhancement of GABAergic inhibition is thought to impact many vital brain functions and interferes with the genesis and/or progression of numerous brain disorders. Here, we show that selectively increasing nMDA receptor activity in inhibitory neurons using an nMDAR positive allosteric modulator (pAM) elevates spiking activity of inhibitory neurons in vitro and in vivo. in vivo infusion of pAM increases spontaneous and sound-evoked spiking in inhibitory and decreases spiking in excitatory neurons, and increases signal-to-noise ratio in the primary auditory cortex. in addition, pAM infusion prior to noise trauma prevents the occurrence of tinnitus and reduction in GABAergic inhibition. these results reveal that selectively enhancing endogenous nMDAR activity on the GABAergic neurons can effectively enhance inhibitory activity and alter excitatory-inhibitory balance, and may be useful for preventing diseases that involve reduced inhibition as the major cause.

show abstract

Optogenetic Modulation of a Minor Fraction of Parvalbumin-Positive Interneurons Specifically Affects Spatiotemporal Dynamics of Spontaneous and Sensory-Evoked Activity in Mouse Somatosensory Cortex in Vivo

Cited by 35 publications

References 64 publications

Navigating the translational roadblock: Towards highly specific and effective all-optical interrogations of neural circuits

Navigating the translational roadblock: Towards highly specific and effective all-optical interrogations of neural circuits

Repetitive transcranial magnetic stimulation recovers cortical map plasticity induced by sensory deprivation due to deafferentiation

Increasing endogenous activity of NMDARs on GABAergic neurons increases inhibition, alters sensory processing and prevents noise-induced tinnitus

Contact Info

Product

Resources

About