Optogenetic induction of hibernation-like state with modified human Opsin4 in mice

Takahashi, Tohru; Hirano, Arisa; Kanda, Takeshi; Saito, Viviane M.; Ashitomi, Hiroto; Tanaka, Kazumasa Z.; Yokoshiki, Yasufumi; Masuda, Kosaku; Yanagisawa, Masashi; Vogt, Kaspar E.; Tokuda, Takashi; Sakurai, Takeshi

doi:10.1016/j.crmeth.2022.100336

Cited by 8 publications

(1 citation statement)

References 67 publications

(102 reference statements)

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“…The characteristic lower intensity and longer duration light-inducibility is advantageous for in vivo applications, since it will cause less heat generation and less phototoxicity 20,21 . Furthermore, lower intensity light inducibility might presumably be applicable for optogenetic manipulation without intracranial surgery like ChRmine and OPN4dC 22,23 . The LSL-ACR2 mouse strain will provide a new opportunity for researchers to use the e cient inhibitory optogenetic tool.…”

Section: Discussionmentioning

confidence: 99%

Cre-dependent ACR2-expressing reporter mouse strain for efficient long-lasting inhibition of neuronal activity

Mukai

Nakamura

et al. 2023

Preprint

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Optogenetics is a powerful tool for manipulating neuronal activity by light illumination with high temporal and spatial resolution. Anion-channelrhodopsins (ACRs) are light-gated anion channels that allow researchers to efficiently inhibit neuronal activity. A blue light-sensitive ACR2 has recently been used in several in vivo studies; however, the reporter mouse strain expressing ACR2 has not yet been reported. Here, we generated a new reporter mouse strain, LSL-ACR2, in which ACR2 is expressed under the control of Cre recombinase. We crossed this strain with a noradrenergic neuron-specific driver mouse (NAT-Cre) to generate NAT-ACR2 mice. We confirmed Cre-dependent expression and function of ACR2 in the targeted neurons by immunohistochemistry and electrophysiological recordings in vitro, and confirmed physiological function using an in vivo behavioral experiment. Our results show that the LSL-ACR2 mouse strain can be applied for optogenetic inhibition of targeted neurons, particularly for long-lasting continuous inhibition, upon crossing with Cre-driver mouse strains. The LSL-ACR2 strain can be used to prepare transgenic mice with homogenous expression of ACR2 in targeted neurons with a high penetration ratio, good reproducibility, and no tissue invasion.

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