Optogenetic control of endogenous Ca2+ channels in vivo

Kyung, Taeyoon; Lee, Sangkyu; Kim, Jung Eun; Cho, Taesup; Park, Hye-Rim; Jeong, Yun-Mi; Kim, Dongkyu; Shin, Anna; Kim, Sung‐Soo; Baek, Jong Hwan; Kim, Jihoon; Kim, Na Yeon; Woo, Doyeon; Chae, Sujin; Kim, Cheol-Hee; Shin, Hee‐Sup; Han, Yong‐Mahn; Kim, Daesoo; Heo, Won Do

doi:10.1038/nbt.3350

Cited by 142 publications

(148 citation statements)

References 43 publications

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“…In a similar manner, light-induced plasma membrane translocation of specific phospholipid kinase and phosphatase domains has been demonstrated to modulate endocytosis and actin dynamics (38, 56, 86–88). A particularly unique application of optogenetic plasma membrane recruitment is the control of calcium (Ca2+) influx (89–91), which has been used to study memory formation in the mouse hippocampus (91). In an optogenetic strategy similar to CLICR (65), light-induced oligomerization of Cry2-fused STIM1 activated the endogenous CRAC calcium channel family and stimulated an intracellular calcium influx.…”

Section: Applicationsmentioning

confidence: 99%

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At Light Speed: Advances in Optogenetic Systems for Regulating Cell Signaling and Behavior

Repina

Rosenbloom

Mukherjee

et al. 2017

Annu. Rev. Chem. Biomol. Eng.

134

117

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Cells are bombarded by extrinsic signals that dynamically change in time and space. Such dynamic variations can exert profound effects on behaviors, including cellular signaling, organismal development, stem cell differentiation, normal tissue function, and disease processes such as cancer. Although classical genetic tools are well suited to introduce binary perturbations, new approaches were necessary to investigate how dynamic signal variation may regulate cell behavior. This fundamental question is increasingly being addressed with optogenetics, a field focused on engineering and harnessing light-sensitive proteins to interface with cellular signaling pathways. Channelrhodopsins defined optogenetics; however, through recent use of light-responsive proteins with myriad spectral and functional properties, practical applications of optogenetics currently encompass cell signaling, subcellular localization, and gene regulation. Now, important questions regarding signal integration within branch points of signaling networks, asymmetric cell responses to spatially restricted signals, and effects of signal dosage versus duration can be addressed. This review summarizes emerging technologies and applications within the expanding field of optogenetics.

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Section: Applicationsmentioning

confidence: 99%

“…This OptoSTIM1 system was expressed and activated in hippocampal excitatory neurons of wild-type mice that subsequently received either an auditory or a foot-shock stimulus. Investigators found that this increased calcium stimulated contextual fear memory in foot-shocked mice (91). …”

Section: Applicationsmentioning

confidence: 99%

At Light Speed: Advances in Optogenetic Systems for Regulating Cell Signaling and Behavior

Repina

Rosenbloom

Mukherjee

et al. 2017

Annu. Rev. Chem. Biomol. Eng.

134

117

View full text Add to dashboard Cite

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“…The recent development of optogenetic probes to activate SOCE may be an important step in this direction [15].…”

Section: +mentioning

confidence: 99%

Neuronal SOCE: Myth or Reality?

Lü

Fivaz

2016

Trends in Cell Biology

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“…The role of STIM1 in control of neuronal L-type Ca 2+ channel activity [10] and feedback regulation of Ca 2+ signals in presynaptic terminals was reported [11]. Using optically controlled construct OptoSTIM1, Kyung et al demonstrated that expression and activation of this construct in mouse hippocampus is able to induce nSOC Ca 2+ influx and promote contextual memory formation [12]. Majewski et al observed improvements of contextual learning in STIM1 overexpressing mice [13].…”

mentioning

confidence: 99%

“…Observed discrepancies are likely to be caused by differences in experimental models and protocols. Nevertheless, learning and synaptic plasticity phenotypes observed in STIM1 and STIM2 knockout and overexpression models [12][13][14]16] suggest that these proteins clearly play a role in neuronal function. It is likely that the main role of nSOC in neurons is signaling and not just refilling of the stores [23].…”

mentioning

confidence: 99%

STIM proteins as regulators of neuronal store-operated calcium influx

Попугаева

Bezprozvanny

2018

Neurodegenerative Disease Management

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The store-operated calcium channels (SOC) were initially described in nonexcitable cells, such as T cells in the immune system [1]. In neurons, the presence of SOC has been controversial [2]. The main argument against neuronal SOC (nSOC) is that neurons possess many other calcium-permeable channels with high conductance, such as NMDA receptors and voltage-gated calcium channels. The existence, functional relevance and molecular identity of nSOC are still under investigation. However, recent molecular analysis of nSOC provided support to its existence and importance for neuronal function.SOC is mediated by two main components: endoplasmic reticulum (ER) Ca 2+ sensors STIM1 or STIM2 proteins [3] and plasma membrane (PM) Ca 2+ channels from Orai and TRPC protein families [1]. SOC in nonexcitable cells is activated by ER Ca 2+ depletion in response to activation of inositol (1,4,5)-trisphosphate receptors and Ca 2+ release from the ER. ER Ca 2+ concentration is sensed by helix-loop-helix structural domain (EF hand domain) in the luminal portion of STIM1. Following Ca 2+ depletion from the ER, STIM1 forms oligomers and translocates to ER-PM junctions [1]. Once located to ER-PM junctions, STIM1 binds to Orai proteins and activate SOC Ca 2+ influx, leading to an increase in intracellular Ca 2+ concentration. This cytoplasmic Ca 2+ abundance is sequestered by SERCA pump to the ER, refilling the stores. The EF hand domain of STIM2 proteins has significantly lower affinity for Ca 2+ than STIM1, and it has been established that STIM2 proteins primarily control steady-state ER and cytosolic Ca 2+ homeostasis [4]. Both STIM1 and STIM2 proteins are expressed in hippocampal neurons [5], and both of these proteins have been implicated in control of nSOC. Klejman et al. observed that STIM1 is colocalized with Orai1 upon depletion of Ca 2+ stores in dendrites and soma [6]. More recent reports suggested that STIM1 and Orai1 are colocalized and may interact with synaptopodin, a protein that regulates synaptic plasticity in hippocampal dendritic spines [7]. Activation of STIM1 and nSOC was described in hippocampal neurons following activation of type I metabotropic glutamate receptors (mGluR) or muscarinic acetylcholine receptors (mAChR) [8]. Notably, nSOC-independent functions of STIM1 in hipocampal neurons were also reported. It was suggested that STIM1 directly controls level of phosphorylation and surface expression of the AMPAR [9]. The role of STIM1 in control of neuronal L-type Ca 2+ channel activity [10] and feedback regulation of Ca 2+ signals in presynaptic terminals was reported [11]. Using optically controlled construct OptoSTIM1, Kyung et al. demonstrated that expression and activation of this construct in mouse hippocampus is able to induce nSOC Ca 2+ influx and promote contextual memory formation [12]. Majewski et al. observed improvements of contextual learning in STIM1 overexpressing mice [13]. These authors further demonstrated that overexpression of STIM1 does not influence long-term potentiaton (LTP) induction in...

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Optogenetic control of endogenous Ca2+ channels in vivo

Cited by 142 publications

References 43 publications

At Light Speed: Advances in Optogenetic Systems for Regulating Cell Signaling and Behavior

At Light Speed: Advances in Optogenetic Systems for Regulating Cell Signaling and Behavior

Neuronal SOCE: Myth or Reality?

STIM proteins as regulators of neuronal store-operated calcium influx

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