Optofluidic devices with integrated solid-state nanopores

Liu, Shuo; Hawkins, Aaron R.; Schmidt, Holger

doi:10.1007/s00604-016-1758-y

Cited by 15 publications

(13 citation statements)

References 89 publications

(65 reference statements)

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“…Additionally, graphene-based two-dimensional nanostructures have also attracted a great scientific interest in the recent years [63,64]. Furthermore, the combination of nanomaterials with microfluidics and modern optical sensing techniques has led to the emergence of a novel and rapidly growing interdisciplinary research field, namely optofluidics [65,66], which has significantly increased detection sensitivity and reduced the detection limit for various biosensors [67]. The surface-enhanced Raman spectroscopy (SERS) is an ideal example of a detection method that has considerably benefitted from the integration of optical nanosensors within microfluidic devices [68,69].…”

Section: Design and Working Principlesmentioning

confidence: 99%

Biosensors-on-Chip: An Up-to-Date Review

et al. 2020

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Generally, biosensors are designed to translate physical, chemical, or biological events into measurable signals, thus offering qualitative and/or quantitative information regarding the target analytes. While the biosensor field has received considerable scientific interest, integrating this technology with microfluidics could further bring significant improvements in terms of sensitivity and specificity, resolution, automation, throughput, reproducibility, reliability, and accuracy. In this manner, biosensors-on-chip (BoC) could represent the bridging gap between diagnostics in central laboratories and diagnostics at the patient bedside, bringing substantial advancements in point-of-care (PoC) diagnostic applications. In this context, the aim of this manuscript is to provide an up-to-date overview of BoC system development and their most recent application towards the diagnosis of cancer, infectious diseases, and neurodegenerative disorders.

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Section: Design and Working Principlesmentioning

confidence: 99%

Biosensors-on-Chip: An Up-to-Date Review

et al. 2020

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“…Amit Meller's group simultaneously monitored optical and electrical signals of a strand of fluorescently labeled DNA transiting a nanopore, and a similar approach may allow for selective resistive pulse analyses in complex protein samples. Optical methods can even replace electrical measurements to monitor ionic current through a nanopore, as has been shown with calcium‐flux sensing on nanopore arrays . The challenge is, however, to collect a sufficient number of photons during the short‐lived dwell times (µs) of proteins through nanopores.…”

Section: Challenges and Outlookmentioning

confidence: 99%

Nanopore‐Based, Rapid Characterization of Individual Amyloid Particles in Solution: Concepts, Challenges, and Prospects

Mayer

List

2018

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Aggregates of misfolded proteins are associated with several devastating neurodegenerative diseases. These so-called amyloids are therefore explored as biomarkers for the diagnosis of dementia and other disorders, as well as for monitoring disease progression and assessment of the efficacy of therapeutic interventions. Quantification and characterization of amyloids as biomarkers is particularly demanding because the same amyloid-forming protein can exist in different states of assembly, ranging from nanometer-sized monomers to micrometer-long fibrils that interchange dynamically both in vivo and in samples from body fluids ex vivo. Soluble oligomeric amyloid aggregates, in particular, are associated with neurotoxic effects, and their molecular organization, size, and shape appear to determine their toxicity. This concept article proposes that the emerging field of nanopore-based analytics on a single molecule and single aggregate level holds the potential to account for the heterogeneity of amyloid samples and to characterize these particles-rapidly, label-free, and in aqueous solution-with regard to their size, shape, and abundance. The article describes the concept of nanopore-based resistive pulse sensing, reviews previous work in amyloid analysis, and discusses limitations and challenges that will need to be overcome to realize the full potential of amyloid characterization on a single-particle level.

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“…Nanopores in solid-state membranes such as SiN have the advantage that their size and shape can be defined over a wide range and thus adapted to a specific nanoparticle. Successful integration of a nanopore in a silicon-based ARROW chip was demonstrated a few years ago, but recently this approach was combined with the aforementioned single particle optical detection to implement dual-mode correlated sensing of individual nanoparticles such as DNAs and virus particles [153–155]. This shows that optofluidic biosensing can be integrated and advanced further by adding other detection modalities.…”

Section: Optofluidic Biosensingmentioning

confidence: 99%

Optofluidic bioanalysis: fundamentals and applications

et al. 2017

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Over the past decade, optofluidics has established itself as a new and dynamic research field for exciting developments at the interface of photonics, microfluidics, and the life sciences. The strong desire for developing miniaturized bioanalytic devices and instruments, in particular, has led to novel and powerful approaches to integrating optical elements and biological fluids on the same chip-scale system. Here, we review the state-of-the-art in optofluidic research with emphasis on applications in bioanalysis and a focus on waveguide-based approaches that represent the most advanced level of integration between optics and fluidics. We discuss recent work in photonically reconfigurable devices and various application areas. We show how optofluidic approaches have been pushing the performance limits in bioanalysis, e.g. in terms of sensitivity and portability, satisfying many of the key requirements for point-of-care devices. This illustrates how the requirements for bianalysis instruments are increasingly being met by the symbiotic integration of novel photonic capabilities in a miniaturized system.

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Optofluidic devices with integrated solid-state nanopores

Cited by 15 publications

References 89 publications

Biosensors-on-Chip: An Up-to-Date Review

Biosensors-on-Chip: An Up-to-Date Review

Nanopore‐Based, Rapid Characterization of Individual Amyloid Particles in Solution: Concepts, Challenges, and Prospects

Optofluidic bioanalysis: fundamentals and applications

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