Optofluidic cell manipulation for a biological microbeam

Grad, Michael; Bigelow, Alan W.; Garty, Guy; Attinger, Daniel; Brenner, David J.

doi:10.1063/1.4774043

Cited by 10 publications

(5 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…31–36 Rapid electrokinetic patterning (REP) 31,34 is such an emerging particle manipulation method. REP uses a combination of electrokinetic and hydrodynamic effects to achieve trapping.…”

Section: Introductionmentioning

confidence: 99%

“…30 Recently optoelectric methods have emerged that offer a similar level of dynamic control as optical tweezers in trapping and manipulating particles. [31][32][33][34][35][36] Rapid electrokinetic patterning (REP) 31,34 is such an emerging particle manipulation method. REP uses a combination of electrokinetic and hydrodynamic effects to achieve trapping.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Trapping and viability of swimming bacteria in an optoelectric trap

et al. 2016

View full text Add to dashboard Cite

Non-contact manipulation methods capable of trapping and transporting swimming bacteria can significantly aid in chemotaxis studies. However, high swimming speed makes the trapping of these organisms an inherently challenging task. We demonstrate that an optoelectric technique, rapid electrokinetic patterning (REP), can effectively trap and manipulate Enterobacter aerogenes bacteria swimming at velocities greater than 20 μm/s. REP uses electro-orientation, laser-induced AC electrothermal flow, and particle-electrode interactions for capturing these cells. In contrast to trapping non-swimming bacteria and inert microspheres, we observe that electro-orientation is critical to the trapping of the swimming cells, since unaligned bacteria can swim faster than the radially inward electrothermal flow and escape the trap. By assessing the cell membrane integrity, we study the effect of REP trapping conditions, including optical radiation, laser-induced heating, and the electric field on cell viability. When applied individually, the optical radiation and laser-induced heating have negligible effect on cells. At the standard REP trapping conditions fewer than 2% of cells have a compromised membrane after four minutes. To our knowledge this is the first study detailing the effect of REP trapping on cell viability. The presented results provide a clear guideline on selecting suitable REP parameters for trapping living bacteria.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Trapping and viability of swimming bacteria in an optoelectric trap

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Columbia University's RARAF facility has been designing, developing, and using focused ion beams for biological irradiation for more than 30 years 17, [23][24][25][26][27][28][29][30][31][32][33][34][35] . The focusing systems have used quadrupole focusing elements in electrostatic triplets and quadruplets 24,33 and permanent magnets 26 .…”

Section: Optimizing Ion Beam Focusing Within the Irradiation Platformmentioning

confidence: 99%

Focused ion beam irradiation platform with a 3-D fluorescence imaging system: a flexible tool for cancer research

Harken,

Deoli,

Campos

et al. 2023

Preprint

View full text Add to dashboard Cite

To improve particle radiotherapy, we need a better understanding of the biology of targeted and non-targeted radiation effects. This is particularly important in the field of heavy ion radiation therapy where global effects are observed, yet only a subset of cells receive a high energy deposition from ion tracks. To allow real-time 3D imaging of living biological samples during and after irradiation with a focused ion beam, we have integrated a high-speed light sheet fluorescence imaging system (swept confocally aligned planar excitation, SCAPE microscopy) into the focused ion beam irradiation platform (FIBIP) at the Columbia University Radiological Research Accelerator Facility (RARAF). The RARAF FIBIP is centered on a 7 T superconducting solenoid magnet, which focuses an ion beam, generated by RARAF’s ion accelerators, to a field size of between 5 mm and 2 µm. An integrated SCAPE volumetric imaging system with associated environmental control allows sub-second observation of 3D cell cultures sample over extended periods before, during, and after radiation treatments. The developed system enables exploration of the mechanistic effects of ion radiotherapy at the cellular and tissue levels. Here, we report details of the integrated system, and show an example of intracellular calcium signaling following ionizing radiation in U87 human glioblastoma 3D cultures using the integrated system.

show abstract

“…Through the creation of different light patterns, multiple particles can be manipulated simultaneously for various functions like cell sorting [10], storing [11] and forming different microparticle patterns [12]. This technique can be applied to both nonbiological particle [13] and biological cell [14]- [16].…”

Section: Introductionmentioning

confidence: 99%

Optimization of a Single-Particle Micropatterning System With Robotic nDEP-Tweezers

Huang

Cui

Lai

et al. 2022

IEEE Trans. Automat. Sci. Eng.

View full text Add to dashboard Cite

In this study, a system of automatic microparticle patterning that could enable the separation, trapping, and translation of single microbeads in liquid suspension using negative dielectrophoresis (DEP) tweezers was presented to form a singlebead pattern. A microchip with integrated electrodes was flipped and placed above the substrate through a micromanipulator. Microparticles laying on the substrate could be displaced to different positions relative to the electrodes on the microchip, and only the selected particles would be trapped by the electric fields generated from electrodes. Vision-based approaches were used to evaluate the necessary information such as the gap distance and the positions of electrodes and microparticles in the image. A strategy for separating nearby particles was proposed to achieve single-bead patterning with high accuracy. A controller was used to guide the microparticles toward the position for trapping while avoiding flow disturbance. Different strategies were simulated to decrease the patterning time and find the minimum traveling distance and find the best route of movement. The optimization problem is NP-hard. Hence, global optimization algorithms such as genetic algorithm, particle swarm optimization, and ant colony optimization (ACO), were simulated, and the results were compared with those of the local optimization method. The comparison results showed that ACO obtained the best performance among the methods. The strategy for constructing high-quality microparticle patterns was also examined through experiments. Orange fluorescent polystyrene beads suspended in 6-aminohexanoic acid solution were considered and successfully patterned on a glass substrate by using the proposed system.Note to Practitioners-Micropatterning is an effective tool for pharmaceutical research and drug discovery. However, the reliability of results depends on the quality of patterns. Existing approaches such as microfluidic devices are limited to create a pattern from one chip for single use and the entire process is sealed and isolated from the environment. In this study, a multi-electrode microchip combined with a vision-based micromanipulator is introduced to create a novel non-contact approach for microparticle patterning, which offers high flexibility and guarantees the quality of the constructed patterns. The electrodes on the chip can be selectively energized to determine the shape of the final pattern. A real-time screening is performed so that the micromanipulator will only guide the particles in good condition for selection. An optimization algorithm is implemented to aid the particle selection with the electrodes, allowing high-quality microparticle patterns to be constructed in a short time for various applications.

show abstract

Optofluidic cell manipulation for a biological microbeam

Cited by 10 publications

References 29 publications

Trapping and viability of swimming bacteria in an optoelectric trap

Trapping and viability of swimming bacteria in an optoelectric trap

Focused ion beam irradiation platform with a 3-D fluorescence imaging system: a flexible tool for cancer research

Optimization of a Single-Particle Micropatterning System With Robotic nDEP-Tweezers

Contact Info

Product

Resources

About