Optoacoustic sensor for nanoparticle linked immunosorbent assay (NanoLISA)

Conjusteau, André; Liopo, Anton V.; Tsyboulski, Dmitri A.; Ermilov, Sergey A.; Elliott, W.R.; Barsalou, Norman; Maswadi, Saher; Glickman, Randolph D.; Oraevsky, Alexander A.

doi:10.1117/12.879401

Cited by 8 publications

(9 citation statements)

References 21 publications

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“…GNR can absorb light about one thousand times more strongly than an equivalent volume of an organic dye [6,10]. Consequently, GNR have been employed in diagnostics [11], therapeutic-delivery systems [12], including small-molecule drugs [13] and exogeneous nucleic acids [14], imaging [15,16], sensing [17], and responsive advanced material assemblies [18]. Properties such as biocompatibility ease of functionalization, and near infrared optical imaging make gold nanorods promising in new theranostic platforms [4].…”

Section: Introductionmentioning

confidence: 99%

Biocompatible Gold Nanorod Conjugates for Preclinical Biomedical Research

Liopo

Conjusteau²,

Tsyboulski³

et al. 2012

J Nanomedic Nanotechnol

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Gold nanorods with a peak absorption wavelength of 760 nm were prepared using a seed-mediated method. A novel protocol has been developed to replace hexadecyltrimethylammonium bromide on the surface of the nanorods with 16-mercaptohexadecanoic acid and metoxy-poly(ethylene glycol)-thiol, and the monoclonal antibody HER2. The physical chemistry properties of the conjugates were monitored through optical and zeta-potential measurements to confirm surface chemistry changes. The efficiency of the modifications was quantified through measurement of the average number of antibodies per gold nanorod. The conjugates were investigated for different cells lines: BT-474, MCF7, MCF10, MDCK, and fibroblast. The results show successful cell accumulation of the gold nanorod HER2 conjugates in cells with HER2 overexpression. Incubation of the complexes in heparinized mouse blood demonstrated the low aggregation of the metallic particles through stability of the spectral properties, as verified by UV/VIS spectrometry. Cytotoxicity analysis with LDH release and MTT assay confirms strong targeting and retention of functional activity of the antibody after their conjugation with gold nanorods. Silver staining confirms efficient specific binding to BT-474 cells even in cases where the nanorod complexes were incubated in heparinized mouse blood. This is confirmed through in vivo studies where, following intravenous injection of gold nanorod complexes, silver staining reveals noticeably higher rates of specific binding in mouse tumors than in healthy liver. The conjugates are reproducible, have strong molecular targeting capabilities, have long term stability in vivo and can be used in pre-clinical applications. The conjugates can also be used for molecular and optoacoustic imaging, quantitative sensing of biological substrates, and photothermal therapy.

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Section: Introductionmentioning

confidence: 99%

Biocompatible Gold Nanorod Conjugates for Preclinical Biomedical Research

Liopo

Conjusteau²,

Tsyboulski³

et al. 2012

J Nanomedic Nanotechnol

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“…The photoacoustic signal from each sample was detected using a probe beam deflection technique. For that, the change in the direction of a probe laser beam was monitored with a position sensitive detector to detect the photoacoustic signal from the sample that causes the refractive index gradient in the water tank. shows a schematic diagram of the system we built for the photoacoustic signal measurements [25,26]. The excitation source was from a tunable optical parametric oscillator (OPO) (Opolette HR 355 II), which has a pulse duration of ~5 ns, a wavelength tunable from 410 to 2200 nm, a repetition rate of 1 - 20 Hz, and a maximum pulse energy of 3 mJ.…”

Section: Methodsmentioning

confidence: 99%

Photoacoustic emission from fluorescent nanodiamonds enhanced with gold nanoparticles

Zhang¹,

Fang²,

Chang³

et al. 2012

Biomed. Opt. Express

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Fluorescent nanodiamonds (FNDs) have drawn much attention in recent years for biomedical imaging applications due to their desired physical properties including excellent photostability, high biocompatibility, extended far-red fluorescence emission, and ease of surface functionalization. Here we explore a new feature of FNDs, i.e. their photoacoustic emission capability, which may lead to potential applications of using FNDs as a dual imaging contrast agent for combined fluorescence and photoacoustic imaging modalities. We observed significant enhancement of photoacoustic emission from FNDs when they were conjugated with gold nanoparticles (GNPs).

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“…We have previously reported on the development of an optoacoustic biosensor intended for the detection of bloodborne microorganisms using immunoaffinity reactions of antibody-coupled gold nanorods as contrast agents specifically targeted to the antigen of interest [30][31][32] . The sensitivity of Nano-LISA is at least OD=10 -6 which allows reliable detection of 1 pg/ml (depending on the commercial antibodies that are used).…”

Section: Nanolisa Prototypementioning

confidence: 99%

PEG-coated gold nanorod monoclonal antibody conjugates in preclinical research with optoacoustic tomography, photothermal therapy, and sensing

2012

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Gold nanorods (GNR) with a peak absorption wavelength of 760 nm were prepared using a seed-mediated method. A novel protocol has been developed to replace hexadecyltrimethylammonium bromide (CTAB) on the surface of GNR with 16-mercaptohexadecanoic acid (MHDA) and metoxy-poly(ethylene glycol)-thiol (PEG), and the monoclonal antibodies: HER2 or CD33. The physical chemistry property of the conjugates was monitored through optical and zetapotential measurements to confirm surface chemistry. The plasmon resonance is kept in the near infrared area, and changes from strong positive charge for GNR-CTAB to slightly negative for GNR-PEG-mAb conjugates are observed. The conjugates were investigated for different cells lines: breast cancer cells and human leukemia lines in vivo applications. These results demonstrate successful tumor accumulation of our modified PEG-MHDA conjugates of GNR for HER2/neu in both overexpressed breast tumors in nude mice, and for thermolysis of human leukemia cells in vitro. The conjugates are non-toxic and can be used in pre-clinical applications, as well as molecular and optoacoustic imaging, and quantitative sensing of biological substrates.

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Optoacoustic sensor for nanoparticle linked immunosorbent assay (NanoLISA)

Cited by 8 publications

References 21 publications

Biocompatible Gold Nanorod Conjugates for Preclinical Biomedical Research

Biocompatible Gold Nanorod Conjugates for Preclinical Biomedical Research

Photoacoustic emission from fluorescent nanodiamonds enhanced with gold nanoparticles

PEG-coated gold nanorod monoclonal antibody conjugates in preclinical research with optoacoustic tomography, photothermal therapy, and sensing

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