Opto-electrokinetic manipulation for high-performance on-chip bioassays

Kwon, Jae‐Sung; Ravindranath, Sandeep P.; Kumar, Aloke; Irudayaraj, Joseph; Wereley, Steven T.

doi:10.1039/c2lc40662d

Cited by 31 publications

(32 citation statements)

References 35 publications

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“…It has been steadily applied for concentration, trapping, patterning, and separation of micro-and nano-particles ranging from 22 nm to 10 µm, establishing its firm position in colloidal manipulation [25,58,[61][62][63]109,[127][128][129]. Recently, REP was also used for manipulation of swimming bacteria [130] and carbon nanotubes [131], extending its applicability in various fields including biotechnology.…”

Section: Current Challenges Of Repmentioning

confidence: 99%

“…Through coupling of the effects, REP can manipulate a large quantity of micro-and nano-sized particles at once at an arbitrary location in a microfluidic chip. The capability has been demonstrated by various experiments (Figure 6) [25,58,[61][62][63]109]. In this section, we introduce the various aspects of REP, including the underlying principle, operating environment, and current challenges.…”

Section: Rapid Electrokinetic Patterningmentioning

confidence: 99%

“…Generally, REP is realized in a simple microfluidic chip structure consisting of two parallel plate electrodes, a microchannel, and inlet and outlet reservoirs (Figure 7) [25,58,[61][62][63][64]. In the REP chip, a transparent indium tin oxide (ITO)-coated glass substrate is used to create a pair of electrodes for particle flow visualization, imaging, and laser irradiation.…”

Section: Driving Environment Of Repmentioning

confidence: 99%

“…The particles were aggregated, and their clusters were translated across the electrode surface by dynamically controlling the location of the focused laser. Kwon et al first demonstrated the bio-compatibility of REP with several kinds of microorganisms [24,25,64]. A large quantity of motile Shewanella oneidensis MR-1 bacteria was trapped, concentrated, and transported using the technique.…”

Section: Introductionmentioning

confidence: 99%

“…A main focus is on how precisely and fast cell manipulation can be performed in a non-invasive manner [3,[24][25][26]. To date, various microfluidic manipulation techniques to meet the requirements have been developed and integrated into a chip for high-resolution, high-throughput, and non-contaminating biological assays.…”

Section: Introductionmentioning

confidence: 99%

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Microfluidic Technology for Cell Manipulation

Kwon

2018

Applied Sciences

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Featured Application: Microfluidic manipulation techniques not only improve the efficiency and reliability of biological/clinical analysis, but also further enable the development of a high-performance bioassay system. Abstract: Microfluidic techniques for cell manipulation have been constantly developed and integrated into small chips for high-performance bioassays. However, the drawbacks of each of the techniques often hindered their further advancement and their wide use in biotechnology. To overcome this difficulty, an examination and understanding of various aspects of the developed manipulation techniques are required. In this review, we provide the details of primary microfluidic techniques that have received much attention for bioassays. First, we introduce the manipulation techniques using a sole driving source, i.e., dielectrophoresis, electrophoresis, optical tweezers, magnetophoresis, and acoustophoresis. Next, we present rapid electrokinetic patterning, a hybrid opto-electric manipulation technique developed recently. It is introduced in detail along with the underlying physical principle, operating environment, and current challenges. This paper will offer readers the opportunity to improve existing manipulation techniques, suggest new manipulation techniques, and find new applications in biotechnology.

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Section: Current Challenges Of Repmentioning

confidence: 99%

Section: Rapid Electrokinetic Patterningmentioning

confidence: 99%

Section: Driving Environment Of Repmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microfluidic Technology for Cell Manipulation

Kwon

2018

Applied Sciences

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Optoelectric patterning: Effect of electrode material and thickness on laser‐induced AC electrothermal flow

et al. 2015

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Rapid electrokinetic patterning (REP) is an emerging optoelectric technique that takes advantage of laser-induced AC electrothermal flow and particle-electrode interactions to trap and translate particles. The electrothermal flow in REP is driven by the temperature rise induced by the laser absorption in the thin electrode layer. In previous REP applications 350-700 nm indium tin oxide (ITO) layers have been used as electrodes. In this study, we show that ITO is an inefficient electrode choice as more than 92% of the irradiated laser on the ITO electrodes is transmitted without absorption. Using theoretical, computational, and experimental approaches, we demonstrate that for a given laser power the temperature rise is controlled by both the electrode material and its thickness. A 25-nm thick Ti electrode creates an electrothermal flow of the same speed as a 700-nm thick ITO electrode while requiring only 14% of the laser power used by ITO. These results represent an important step in the design of low-cost portable REP systems by lowering the material cost and power consumption of the system.

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On‐Chip Generation of Vortical Flows for Microfluidic Centrifugation

Ahmed

Ramesan

Lee

et al. 2019

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Microcentrifugation constitutes an important part of the microfluidic toolkit in a similar way that centrifugation is crucial to many macroscopic procedures, given that micromixing, sample preconcentration, particle separation, component fractionation, and cell agglomeration are essential operations in small scale processes. Yet, the dominance of capillary and viscous effects, which typically tend to retard flow, over inertial and gravitational forces, which are often useful for actuating flows and hence centrifugation, at microscopic scales makes it difficult to generate rotational flows at these dimensions, let alone with sufficient vorticity to support efficient mixing, separation, concentration, or aggregation. Herein, the various technologies—both passive and active—that have been developed to date for vortex generation in microfluidic devices are reviewed. Various advantages or limitations associated with each are outlined, in addition to highlighting the challenges that need to be overcome for their incorporation into integrated microfluidic devices.

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Opto-electrokinetic manipulation for high-performance on-chip bioassays

Cited by 31 publications

References 35 publications

Microfluidic Technology for Cell Manipulation

Microfluidic Technology for Cell Manipulation

Optoelectric patterning: Effect of electrode material and thickness on laser‐induced AC electrothermal flow

On‐Chip Generation of Vortical Flows for Microfluidic Centrifugation

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