Optischen Abbildung Unter Überschreitung der Beugungsbedingten Auflösungsgrenze

Lukosz, W.; Marchand, Mark

doi:10.1080/713817795

Cited by 84 publications

(56 citation statements)

References 8 publications

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“…In [1] the operation of the technique in terms of structured illumination is explained [6][7][8][9]. In [3], on the other hand, the method as a confocal microscope with a detector array is explained.…”

mentioning

confidence: 99%

Superresolution by image scanning microscopy using pixel reassignment

2013

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The effect of detector array size on resolution and signal collection efficiency of image scanning microscopy based on pixel reassignment is studied. It is shown how the method can also be employed if there is a Stokes shift in fluorescence emission wavelength. With no Stokes shift, the width of the point spread function can be sharpened by a factor of 1.53, and its peak intensity increased by a factor of 1.84.

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mentioning

confidence: 99%

Superresolution by image scanning microscopy using pixel reassignment

2013

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“…This is a special case of structured illumination, which includes other examples such as full-¯eld illumination with a pattern of fringes. 43,44 Structured illumination has been shown to give superior 3D resolution compared with CM, 45,46 but has been found to not penetrate so well into scattering media.…”

Section: Confocal Sectioningmentioning

confidence: 99%

Advanced optical microscopy methods for in vivo imaging of sub-cellular structures in thick biological tissues

Chen

Rehman

Sheppard

2014

J. Innov. Opt. Health Sci.

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Optical microscopy has become an indispensable tool for visualizing sub-cellular structures and biological processes. However, scattering in biological tissues is a major obstacle that prevents high-resolution images from being obtained from deep regions of tissue. We review common techniques, such as multiphoton microscopy (MPM) and optical coherence microscopy (OCM), for di®raction limited imaging beyond an imaging depth of 0.5 mm. Novel implementations have been emerging in recent years giving higher imaging speed, deeper penetration, and better image quality. Focal modulation microscopy (FMM) is a novel method that combines confocal spatial ltering with focal modulation to reject out-of-focus background. FMM has demonstrated an imaging depth comparable to those of MPM and OCM, near-real-time image acquisition, and the capability for multiple contrast mechanisms.

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“…Gustafsson reviewed the idea of Lukosz (1963) 83 and set up a prototype of SIM in 2000. 84 SIM uses patterned light to illuminate the sample and enhances the resolution by measuring the fringes in a Moiré pattern.…”

Section: Dx~lmentioning

confidence: 99%

From microscopy to nanoscopy via visible light

Hao

Kuang

et al. 2013

Light Sci Appl

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The resolution of conventional optical equipment is always restricted by the diffraction limit, and improving on this was previously considered improbable. Optical super-resolution imaging, which has recently experienced rapid growth and attracted increasing global interest, will result in applications in many domains, benefiting fields such as biology, medicine and material research. This review discusses the contributions of different researchers who identified the diffractive barrier and attempted to realize optical super-resolution. This is followed by a personal viewpoint of the development of optical nanoscopy in recent decades and the road towards the next generation of optical nanoscopy.

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Optischen Abbildung Unter Überschreitung der Beugungsbedingten Auflösungsgrenze

Cited by 84 publications

References 8 publications

Superresolution by image scanning microscopy using pixel reassignment

Superresolution by image scanning microscopy using pixel reassignment

Advanced optical microscopy methods for in vivo imaging of sub-cellular structures in thick biological tissues

From microscopy to nanoscopy via visible light

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