Options for Management of Fusarium Wilt of Strawberry in California

Gordon, Thomas R.; Daugovish, Oleg; Koike, S. T.; Islas, Christina M.; Kirkpatrick, S. C.; Yoshisato, J. A.; Shaw, Douglas V.

doi:10.1080/15538362.2016.1219294

Cited by 14 publications

(15 citation statements)

References 5 publications

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“…We identified a dominant gene ( Fw1 ) in octoploid strawberry that confers resistance to a virulent isolate of F. oxysporum f. sp. fragariae found in California ( Gordon et al 2016 ; Henry et al 2017 ). Our findings were consistent with the hypothesis that gene-for-gene resistance to Fusarium wilt might be operating in strawberry, as previously suggested by Mori et al (2005) in a study where a segregating population was screened for resistance to a Japanese isolate of F. oxysporum f. sp.…”

Section: Discussionmentioning

confidence: 99%

“…fragariae (AMP132) immediately before planting. To produce spores (inoculum), the pathogen was grown on potato dextrose agar (PDA) under continuous fluorescent lighting at room temperature for 30 days, as described by Gordon et al (2016) . Spores were freed from the surface by adding sterile deionized (DI) water with 0.5% potassium chloride (KCl) to the growth plates and scraping the edge of the media with a sterile glass slide.…”

Section: Methodsmentioning

confidence: 99%

“…Symptoms had fully progressed and were most extreme among resistant and susceptible checks in the final time point in each year (9-weeks post-inoculation in 2016 and 36-weeks post-inoculation in 2017). We used a previously described 1-5 rating scale to phenotype disease symptoms, where symptomless plants were scored as 1, stunted plants were scored as 2, chlorotic plants were scored as 3, wilting plants were scored as 4, and plants killed by the pathogen were scored as 5 ( Gordon et al 2016 ; Henry et al 2017 ).…”

Section: Methodsmentioning

confidence: 99%

“…Fusarium wilt was first discovered in strawberry in California in 2006 ( Koike et al 2009 ), in fields where fully effective fumigation practices had not been used for several years in succession ( Gordon et al 2016 ). The pathogen has since become more widespread and currently appears to be found throughout the state ( Henry et al 2017 ).…”

mentioning

confidence: 99%

“…The pathogen has since become more widespread and currently appears to be found throughout the state ( Henry et al 2017 ). Consequently, Fusarium wilt has become an increasingly serious threat to strawberry production in California, as in many other parts of the world ( Paynter et al 2014 ; Koike and Gordon 2015 ; Paynter et al 2016 ; Gordon et al 2016 ; Henry et al 2017 ). The development and deployment of resistant cultivars might be the only strategy that can provide reliable and adequate control of Fusarium wilt in strawberry.…”

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confidence: 99%

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Genome-Wide Association Mapping Uncovers Fw1, a Dominant Gene Conferring Resistance to Fusarium Wilt in Strawberry

Pincot¹,

Poorten²,

Hardigan³

et al. 2018

G3 Genes|Genomes|Genetics

108

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Fusarium wilt, a soil-borne disease caused by the fungal pathogen Fusarium oxysporum f. sp. fragariae, threatens strawberry (Fragaria × ananassa) production worldwide. The spread of the pathogen, coupled with disruptive changes in soil fumigation practices, have greatly increased disease pressure and the importance of developing resistant cultivars. While resistant and susceptible cultivars have been reported, a limited number of germplasm accessions have been analyzed, and contradictory conclusions have been reached in earlier studies to elucidate the underlying genetic basis of resistance. Here, we report the discovery of Fw1, a dominant gene conferring resistance to Fusarium wilt in strawberry. The Fw1 locus was uncovered in a genome-wide association study of 565 historically and commercially important strawberry accessions genotyped with 14,408 SNP markers. Fourteen SNPs in linkage disequilibrium with Fw1 physically mapped to a 2.3 Mb segment on chromosome 2 in a diploid F. vesca reference genome. Fw1 and 11 tightly linked GWAS-significant SNPs mapped to linkage group 2C in octoploid segregating populations. The most significant SNP explained 85% of the phenotypic variability and predicted resistance in 97% of the accessions tested—broad-sense heritability was 0.96. Several disease resistance and defense-related gene homologs, including a small cluster of genes encoding nucleotide-binding leucine-rich-repeat proteins, were identified in the 0.7 Mb genomic segment predicted to harbor Fw1. DNA variants and candidate genes identified in the present study should facilitate the development of high-throughput genotyping assays for accurately predicting Fusarium wilt phenotypes and applying marker-assisted selection.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Genome-Wide Association Mapping Uncovers Fw1, a Dominant Gene Conferring Resistance to Fusarium Wilt in Strawberry

Pincot¹,

Poorten²,

Hardigan³

et al. 2018

G3 Genes|Genomes|Genetics

108

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Effect of chemical alternatives to methyl bromide on soil‐borne disease incidence and fungal populations in Spanish strawberry nurseries: A long‐term study

Villarino¹,

Larena²,

Melgarejo³

et al. 2020

Pest Management Science

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BACKGROUND Chloropicrin (PIC) mixtures of 1,3‐dichloropropene and chloropicrin (DD:PIC), dazomet, and metam sodium (MS) have been applied as chemical alternatives to methyl bromide (MB) in Spanish strawberry nurseries since MB was banned as a soil fumigant in 2005. These chemical alternatives were applied to soil in two Spanish strawberry nurseries between 2003 and 2017 to test their efficacy against the main crown and root disease and soil fungal populations in comparison with the use of MB and PIC (MB:PIC). These chemicals were applied at several doses with different application methods under plastic films. Crown and root disease incidence was calculated as the percentage of plants with symptoms caused by soil‐borne pathogens. Soil fungal populations were estimated as colony forming units per gram of dry soil. RESULTS All chemicals significantly reduced soil‐borne fungal disease incidence and fungal population in both nurseries over the years. Phytophthora cactorum and Fusarium spp. were the main pathogens causing soil‐borne diseases, followed by Verticillium spp. MB:PIC remained the treatment that best controlled P. cactorum. MS and DD:PIC controlled Fusarium disease to a lesser extent than MB:PIC and dazomet in both nurseries. MB:PIC and PIC were the two treatments that most reduced Verticillium spp. The population of Verticillium spp. declined and the presence of other species such as Colletotrichum spp. and Rhizoctonia spp. was minimal during the study. CONCLUSION Chemicals are necessary to obtain healthy strawberry plants. The use of chemical alternatives to MB has resulted in changes in the incidence of soil‐borne diseases and soil fungal populations in strawberry nurseries. Dazomet was an effective alternative to MB as a soil‐borne disease control, except against Verticillium spp. MB alternatives in strawberry nursery soils have caused Fusarium spp. to displace Verticillium spp.

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Novel Fusarium wilt resistance genes uncovered in natural and cultivated strawberry populations are found on three non-homoeologous chromosomes

et al. 2022

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Key Message Several Fusarium wilt resistance genes were discovered, genetically and physically mapped, and rapidly deployed via marker-assisted selection to develop cultivars resistant toFusarium oxysporumf. sp.fragariae, a devastating soil-borne pathogen of strawberry. Abstract Fusarium wilt, a soilborne disease caused by Fusarium oxysporum f. sp. fragariae, poses a significant threat to strawberry (Fragaria$$\times$$ × ananassa) production in many parts of the world. This pathogen causes wilting, collapse, and death in susceptible genotypes. We previously identified a dominant gene (FW1) on chromosome 2B that confers resistance to race 1 of the pathogen, and hypothesized that gene-for-gene resistance to Fusarium wilt was widespread in strawberry. To explore this, a genetically diverse collection of heirloom and modern cultivars and octoploid ecotypes were screened for resistance to Fusarium wilt races 1 and 2. Here, we show that resistance to both races is widespread in natural and domesticated populations and that resistance to race 1 is conferred by partially to completely dominant alleles among loci (FW1, FW2, FW3, FW4, and FW5) found on three non-homoeologous chromosomes (1A, 2B, and 6B). The underlying genes have not yet been cloned and functionally characterized; however, plausible candidates were identified that encode pattern recognition receptors or other proteins known to confer gene-for-gene resistance in plants. High-throughput genotyping assays for SNPs in linkage disequilibrium with FW1-FW5 were developed to facilitate marker-assisted selection and accelerate the development of race 1 resistant cultivars. This study laid the foundation for identifying the genes encoded by FW1-FW5, in addition to exploring the genetics of resistance to race 2 and other races of the pathogen, as a precaution to averting a Fusarium wilt pandemic.

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Options for Management of Fusarium Wilt of Strawberry in California

Cited by 14 publications

References 5 publications

Genome-Wide Association Mapping Uncovers Fw1, a Dominant Gene Conferring Resistance to Fusarium Wilt in Strawberry

Genome-Wide Association Mapping Uncovers Fw1, a Dominant Gene Conferring Resistance to Fusarium Wilt in Strawberry

Effect of chemical alternatives to methyl bromide on soil‐borne disease incidence and fungal populations in Spanish strawberry nurseries: A long‐term study

Novel Fusarium wilt resistance genes uncovered in natural and cultivated strawberry populations are found on three non-homoeologous chromosomes

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