Optimum Nutrient Solutions for Plants

Hoagland, D. R.

doi:10.1126/science.52.1354.562

Cited by 131 publications

(63 citation statements)

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“…However, until challenges concerning giant reed breeding and cultivation practices as well as pollution of the environment by emission from direct combustion of giant reed will be solved, bioethanol production from lignocellulose appears to be the best way to utilise the giant reed biomass (Pilu et al 2012). Moreover, properties of this crop including adaptability to high water, salinity, nutrient inputs, and tolerance to pollutants, allow phytoremediation application combined with biomass production (Czakó and Márton 2010 (Murashige and Skoog 1962) with an addition of 2-4 mg/dm 3 of BAP (6-benzylaminopurine) as a cytokinin-type growth regulator for induction of shoots development; (ii) the rooting of regenerated shoots was achieved on MS medium without an addition of growth regulators, and (iii) the pre-cultivation of giant reed seedlings before the experiments was carried out in a diluted nutrient solution (25 % strength) according to Hoagland (1920). The plants were cultivated at a photoperiod of 16 h light / 8 h dark (maximum light intensity was 11 450 lx), temperature of 28 °C (light) / 18 °C (dark) and a relative humidity of 60-80 %, which were provided by the plant growth chamber (KBWF 720;Binder,Germany …”

Section: Introductionmentioning

confidence: 99%

Imaging of photoassimilates transport in plant tissues by positron emission tomography

Partelová¹,

Kuglerová²,

Konotop³

et al. 2017

Nova Biotechnologica Et Chimica

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Section: Introductionmentioning

confidence: 99%

Imaging of photoassimilates transport in plant tissues by positron emission tomography

Partelová¹,

Kuglerová²,

Konotop³

et al. 2017

Nova Biotechnologica Et Chimica

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“…Optimal conditions for HILEP for Arabidopsis plants were established in previous studies ( 14,15 ) based on a modi fi ed Hoagland's hydroponic medium ( 26 ) , which was adapted using other published protocols ( 9,25,27 ) . In the presented study, there was no need to change the hydroponic medium solution for the 7 weeks of cultivation as judged by the plant phenotype.…”

Section: The Peptide Quantitation Is Initiated By Selecting "Analysis\mentioning

confidence: 99%

“…The main strategies employed for the quantitation of proteomes by mass spectrometry are as follows: label-free quantitation (see Chapters 16 ,20 ,and 22 ), chemical labeling (see [17][18][19]and 24 ) or metabolic labeling (see 25 ,and 26 ). In chemical labeling, peptides are typically labeled with isotopically different chemical tags as reviewed by Ong and Mann ( 1 ) (for summary see Chapter 7 ).…”

Section: Introductionmentioning

confidence: 99%

Hydroponic Isotope Labeling of Entire Plants and High-Performance Mass Spectrometry for Quantitative Plant Proteomics

Bindschedler

Mills

Cramer

2012

Methods in Molecular Biology

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Hydroponic isotope labeling of entire plants (HILEP) combines hydroponic plant cultivation and metabolic labeling with stable isotopes using (15)N-containing inorganic salts to label whole and mature plants. Employing (15)N salts as the sole nitrogen source for HILEP leads to the production of healthy-looking plants which contain (15)N proteins labeled to nearly 100%. Therefore, HILEP is suitable for quantitative plant proteomic analysis, where plants are grown in either (14)N- or (15)N-hydroponic media and pooled when the biological samples are collected for relative proteome quantitation. The pooled (14)N-/(15)N-protein extracts can be fractionated in any suitable way and digested with a protease for shotgun proteomics, using typically reverse phase liquid chromatography nanoelectrospray ionization tandem mass spectrometry (RPLC-nESI-MS/MS). Best results were obtained with a hybrid ion trap/FT-MS mass spectrometer, combining high mass accuracy and sensitivity for the MS data acquisition with speed and high-throughput MS/MS data acquisition, increasing the number of proteins identified and quantified and improving protein quantitation. Peak processing and picking from raw MS data files, protein identification, and quantitation were performed in a highly automated way using integrated MS data analysis software with minimum manual intervention, thus easing the analytical workflow. In this methodology paper, we describe how to grow Arabidopsis plants hydroponically for isotope labeling using (15)N salts and how to quantitate the resulting proteomes using a convenient workflow that does not require extensive bioinformatics skills.

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“…Hoagland [14], modified as indicated in Table 2. Separating biological mass from the culture medium through filtration, and washing Unauthenticated Download Date | 5/12/18 11:08 PM it with distilled water allowed to eliminate excessive salts obtained dry biomass.…”

Section: Methodsmentioning

confidence: 99%

Removal of 60Co2+ and 137Cs+ ions from low radioactive solutions using Azolla caroliniana willd. water fern

et al. 2004

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This study concerns the removal of the 137 Cs + and 60 Co 2+ β + γ− radioactive ions in Azolla caroliniana Willd. water fern. The living fern and two different types of biosorbent prepared from Azolla caroliniana were tested to remove the above-mentioned radioactive ions from dilute solutions, in the absence and in the presence of the ionic competition. Effective 137 Cs + and 60 Co 2+ ions removal from low radioactive wastewaters was demonstrated. The time-dependent K d (t) values were calculated from the absorption data. These results indicate that removal process achieved equilibrium in about 120 min and that it involves two steps: rapid and slow absorption; the active process (metabolic bioaccumulation on the living fern) was responsible for above one half of the total removal process. A thin layer radiochromatography study leads to the conclusion that the biochemical components in which 137 Cs + and 60 Co 2+ place themselves are of a polysaccharide and lipoid fractions.

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Optimum Nutrient Solutions for Plants

Cited by 131 publications

References 1 publication

Imaging of photoassimilates transport in plant tissues by positron emission tomography

Imaging of photoassimilates transport in plant tissues by positron emission tomography

Hydroponic Isotope Labeling of Entire Plants and High-Performance Mass Spectrometry for Quantitative Plant Proteomics

Removal of 60Co2+ and 137Cs+ ions from low radioactive solutions using Azolla caroliniana willd. water fern

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