Optimum dietary methionine requirement of juvenile silver pompano, Trachinotus blochii (Lacepede, 1801)

Ebeneezar, Sanal; Vijayagopal, P; Srivastava, Prem Prakash; Gupta, Subodh; Varghese, Tincy; Prabu, D Linga; Chandrasekar, S.; Varghese, Eldho; Tejpal, C.S.; Anikuttan, K K; Sayooj, P; Wilson, Livi

doi:10.1016/j.anifeedsci.2020.114592

Cited by 16 publications

(7 citation statements)

References 52 publications

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“…In the current study, whole-body proximate composition of experimental shes in terms of total ash, calcium and phosphorous content was in uenced by the reduction in the level of inclusion of FM content of the diet and thus the lower values were observed in FM free diet fed shes (100CSM). This is in agreement with Garcia-Abiado et al The essential amino acid pro le of the experimental diets indicating that the levels of limiting amino acids, lysine and methionine were on par with their requirements of lysine (2.4% of diet) and methionine (1.16-1.18% of diet) of snubnose pompano (Ebeneezar et al 2019;Ebeneezar et al 2020). In the present study, the experimental diets were supplemented with external sources of lysine and methionine to maintain their levels equally in all the FM replaced diets on par with the requirements of snubnose pompano.…”

Section: Discussionsupporting

confidence: 91%

Enzymological, Histological and Serum Biomarkers Responses of Snubnose Pompano on Complete Replacement of Fishmeal Using Cottonseed Meal Supplemented With Lysine and Methionine in the Diet

Prabu

Vijayagopal

Ebeneezar

et al. 2021

Preprint

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In a feeding experiment, cottonseed meal (CSM) was used to replace fishmeal (FM) in the diet of snubnose pompano supplemented with lysine and methionine to assess the growth, nutritive profile, hematological, histological and stress biomarkers response. Experimental fishes were randomly stocked in five treatments each with triplicates. Five isonitrogenous and isolipidic diets with graded level of CSM (0, 8.7, 17.4, 26.0 and 34.7%) as replacement for FM protein (0, 25, 50, 75, and 100%) were formulated and fed to respective treatments. Comparison between various parameters among the treatments was made using orthogonal polynomial contrasts to indicate the statistical significance. Higher alkaline phosphatase, acid phosphatase, lactate dehydrogenase, malate dehydrogenase, aspartate and alanine aminotransferase activities were observed in 0CSM group and followed by 100CSM group as higher inclusion level of CSM with higher free gossypol content did not affect the metabolic enzyme activities. The maximum muscular free gossypol accretion of 1.28 mg kg-1 (on wet basis) was recorded in 100CSM group which was very well below the critical limit set by FDA. As a conclusion, fishmeal can be completely replaced using cottonseed meal in the diet of pompano without adverse effect on growth, metabolism and general health.

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Section: Discussionsupporting

confidence: 91%

Enzymological, Histological and Serum Biomarkers Responses of Snubnose Pompano on Complete Replacement of Fishmeal Using Cottonseed Meal Supplemented With Lysine and Methionine in the Diet

Prabu

Vijayagopal

Ebeneezar

et al. 2021

Preprint

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“…The induced lipolysis and β-oxidation not only provide energy for the growth of juvenile blunt snout bream but also may partly contribute to the plasma TG and TC contents that did not show significant differences among the experimental groups. Similar results were also reported in juvenile silver pompano, Trachinotus blochii (Lacepede, 1801) 46 .…”

Section: Discussionsupporting

confidence: 86%

Nutrient metabolism in the liver and muscle of juvenile blunt snout bream (Megalobrama amblycephala) in response to dietary methionine levels

Liang

Ren

et al. 2021

Sci Rep

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A 75-day rearing trial was designed to study the response of juvenile Megalobrama amblycephala to dietary methionine (Met) levels. Three practical diets with graded Met levels (0.40%, 0.84% and 1.28% dry matter) were prepared to feed the juvenile fish. The results showed that the 0.84% Met diet significantly improved the growth compared with 0.40% diets. Compared with 0.84% and 1.28% Met, 0.40% Met significantly increased the hepatic lipid content, while decreasing the muscular lipid and glycogen contents. 0.40% Met decreased the protein levels of phospho-Eukaryotic initiation factor 4E binding protein-1 (p-4e-bp1), 4e-bp1 and Ribosomal protein S6 kinase 1 in the liver, compared with 0.84% diet, while an increasing trend was observed in the muscle. Met supplementation tended to decrease and increase lipid synthesis in the liver and muscle, respectively, via changing mRNA levels of sterol regulatory element-binding protein 1, fatty acid synthetase and acetyl-CoA carboxylase. 1.28% dietary Met promoted fatty acid β-oxidation and lipolysis in both the liver and muscle by increasing carnitine palmitoyl transferase 1, peroxisome proliferator activated receptor alpha, lipoprotein lipase and lipase mRNA levels. Compared with 0.40% and 0.84% dietary Met, 1.28% Met enhanced the mRNA levels of hepatic gluconeogenesis related genes phosphoenolpyruvate carboxykinase (pepck), and glucose-6-phosphatase, and muscular glycolysis related genes phosphofructokinase (pfk), and pyruvate kinase (pk). The mRNA levels of hepatic pfk, pk and glucokinase were markedly downregulated by 1.28% Met compared with 0.84% level. Muscular pepck, glycogen synthase, and hepatic glucose transporters 2 mRNA levels were induced by 1.28% Met. Generally, deficient Met level decreased the growth of juvenile Megalobrama amblycephala, and the different nutrient metabolism responses to dietary Met were revealed in the liver and muscle.

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“…The induced lipolysis and β-oxidation not only provide energy for the growth and Met of blunt snout bream, but also may partly contribute to the plasma TG and TC contents that did not show signi cant differences among the experimental groups. Similar results were shown in juvenile silver pompano, Trachinotus blochii (Lacepede, 1801) [50].…”

Section: 40% Dietary Methionine Increased Hepatic Accumulation While Suppressed Lipogenesis In Muscle In Juvenilemegalobrama Amblycephalasupporting

confidence: 81%

Tissue-specific Nutrient Metabolism in the Liver and Muscle of Juvenile Blunt Snout Bream (Megalobrama Amblycephala) in Response to Dietary Methionine Levels

K¹,

Liang

Ren

et al. 2021

Preprint

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BackgroundMethionine is an essential amino acid, that affects the metabolism of protein, lipid and glucose. However, the metabolic polytrophic response in the liver and muscle of juvenile Megalobrama amblycephala to dietary methionine levels is unclear.ResultsThe 0.84% methionine diet significantly improved the growth performance compared with the 0.40% diet. Dietary methionine levels had no marked effects on plasma parameters or whole body composition of juveniles. The protein levels of phospho-phosphatidylinositol 3-kinase, protein kinase B, phospho-eukaryotic initiation factor 4E binding protein-1 (p-4E-BP1), 4E-BP1 and ribosomal protein S6 kinase 1, in the liver of fish fed the 0.84% diet were higher than those in fish fed the 0.40% diet. While in muscle, these proteins showed the opposite trend. The mRNA levels of the muscular lipid synthesis associated genes: sterol regulatory element-binding protein 1 (SREBP1), fatty acid synthetase (FAS) and acetyl-CoA carboxylase (ACC), were significantly upregulated by the 1.28% methionine diet; while hepatic SREBP1, FAS and ACC mRNA expression levels were increased by 0.40% methionine. In addition, 1.28% dietary methionine significantly induced fatty acid β-oxidation and lipolysis of the liver and muscle via increased carnitine palmitoyl transferase 1, peroxisome proliferator activated receptor alpha, lipoprotein lipase and lipase expression levels. Compared with 0.40% dietary methionine, 1.28% methionine enhanced the mRNA levels of the hepatic gluconeogenesis related genes phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase, and the muscular glycolysis related genes phosphofructokinase (PFK) and pyruvate kinase (PK). The mRNA expression levels of hepatic PFK, PK and glucokinase were markedly upregulated by the 0.84% methionine diet compared with the 1.28% diet. In addition, muscular PEPCK and glycogen synthase, and hepatic glucose transporters 2 mRNA levels were induced by 1.28% methionine. ConclusionThe study showed that optimal methionine levels could enhance the growth of juvenile Megalobrama amblycephala, and the nutrient metabolism response to dietary methionine in the liver and muscle was tissue-specific.

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Optimum dietary methionine requirement of juvenile silver pompano, Trachinotus blochii (Lacepede, 1801)

Cited by 16 publications

References 52 publications

Enzymological, Histological and Serum Biomarkers Responses of Snubnose Pompano on Complete Replacement of Fishmeal Using Cottonseed Meal Supplemented With Lysine and Methionine in the Diet

Enzymological, Histological and Serum Biomarkers Responses of Snubnose Pompano on Complete Replacement of Fishmeal Using Cottonseed Meal Supplemented With Lysine and Methionine in the Diet

Nutrient metabolism in the liver and muscle of juvenile blunt snout bream (Megalobrama amblycephala) in response to dietary methionine levels

Tissue-specific Nutrient Metabolism in the Liver and Muscle of Juvenile Blunt Snout Bream (Megalobrama Amblycephala) in Response to Dietary Methionine Levels

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