Optimum Culture Conditions for Production byPseudomonas chlororaphisB23 of Nitrile Hydratase

Characterization of Nitrile Hydratase Genes Cloned by DNA Screeing fromRhodococcus erythropolis

“…N-774 (I. Watanabe, unpublished results) and Pseudomonas chlororaphis B23,2) which require pyrroloquinoline quinone and a nonheme ion as prosthetic groups for reactions, and the other includes the NHases from Rhodococcus rhodochrous 11, H-and L-NHases, which require only a cobalt ion for their activity.3) In addition, these NHases were inducibly produced by addition of different chemical compounds. The NHase production of P. chlororaphis B23 is induced by methacrylamide 4 ) and the H-and L-NHases of R. rhodochrous J 1 are inducibly produced by urea 5) and cyclohexancarboxamide,3) while the NHase of Rhodococcus sp. N-774 is produced constitutively.…”

Enhancement of enzyme activity and enantioselectivity via cultivation in nitrile metabolism by Rhodococcus sp. CGMCC 0497

“…Various culture conditions play an important role in the formation of desirable enzymes. Several papers have focused on the optimization of culture conditions for different strains capable of nitrile biotransformation, especially the transformation of acrylonitrile to acrylamide [8], a versatile industrial product. However, to our knowledge, few studies have been devoted to the ways in which culture conditions affect enantioselectivity.…”

Building Blocks

“…The criterion for the selection of the strain was the absence of any amidase activity, which would lead to further hydrolysis of the amide to the carboxylic acid. This biotransformation was chosen over the chemical transformation because of the higher conversion, selectivity, and production of higher amounts of product per catalyst weight, as well as less waste [21,22].…”