Optimum Conditions for Measuring Dehydrogenase Activity of Aspergillus niger using TTC

Abstract

ABSTRACT:The suitability of the triphenyl tetrazolium chloride (TTC) for dehydrogenase activity measurement of the vegetative cells of the fungal species Aspergillus niger was investigated. The triphenyl formazan (TF) yield increased with the increases in TTC concentration, pH, temperature and incubation time within the studied ranges. The effects of individual parameters, as well as the combined effects, on the TF yield were found to follow exponential expressions. The sensitivity analysis showed that the TF yield is more sensitive to changes in temperature followed by pH, TTC concentration and incubation time. Although the rate of increase in the enzyme activity decreased gradually with the increase in temperature, no enzyme denaturation was observer below 55 ºC. The optimum TTC-test conditions for measuring the dehydrogenase activity of A. niger vegetative cells are a TTC concentration of 15 g/L, a pH of 9, a temperature of 55 ºC and an incubation time of 4 h. Although the procedure has been successfully tested with mycelia of different stages of growth, it would be interesting to test the effects of different stages of growth on TTC reduction under both aerobic and anaerobic conditions.

Sequential Remediation Processes for Effective Removal of Oil from Contaminated Soils

“…Similar results were reported by Rao et al (1995). Ghaly and Mahmoud (2006) reported a TKN reduction of 55.6% after 60 days during in-vessel bioremediation process of hydrocarbons contaminated soil. Spinelli et al (2005) reported a slower reduction of 25% in TKN (from 10 mg kg −1 −o 7.5 mg kg −1 ) during in-vessel bioremediation of diesel contaminated soil mixed with food industry sludge after 170 days of bioremediation.…”

“…Spinelli et al (2005) reported a slower reduction of 25% in TKN (from 10 mg kg −1 −o 7.5 mg kg −1 ) during in-vessel bioremediation of diesel contaminated soil mixed with food industry sludge after 170 days of bioremediation. Ghaly and Mahmoud (2006) reported 61% reduction in TKN while composting greenhouse tomato plant residues with dairy manure.…”

“…Ghaly et al (2007) reported a small reduction in the moisture content (from 60-59.47%) during the bioremediation of toluene contaminated soil. Ghaly and Mahmoud (2006) reported a reduction in the moisture content from 60.7-59% during composting of green house tomato plant residues with dairy manure. Ma et al (2001) reported 82.69% removal of hydrocarbons at a moisture content within the range of 55-60% during in-vessel bioremediation of petroleum solid waste.…”

The Dynamic of some Biochemical Indicators at Species of Medicinal Plants from Areas of Suceava County, Romania, Emerged under the Mining Pollution Influence

“…Taking into account the fact that the global microbiologic activity of the soil, and implicitly the DH activity -as biomarker of the degradation and reparation processes, are strongly influenced by a series of biotic and abiotic factors as the mining, the time and the temperature of incubation, the type of soil, the season, the loosening/aeration degree of the soil, its pH, the humidity, the management and maintenance practices [73][74][75][76][77] , the soil's fertilization, the deepness profile of the soil, the presence of different heavy metals etc. [78][79][80][81][82] , we resorted at the actual (Dha) and potential (Dha) dehydrogenase activity determination in soil samples taken from two different deepness profiles.…”

Determination of Antimicrobial Potentials of Ethanol Extract of <i>Combretum dolichopentalum</i> Leaves by Total Dehydrogenase Activity Assay

“…They transfer hydrogen and electrons through a chain of intermediate electron carriers to oxygen as a final electron acceptor, which subsequently combine with them to form water [30]. Dehydrogenase activity is largely measured using the triphenyl tetrazolium chloride (TTC) salt as a hydrogen acceptor.…”