Optimizing the Thermal Read-Out Technique for MIP-Based Biomimetic Sensors: Towards Nanomolar Detection Limits

Geerets, Bram; Peeters, Marloes; Grinsven, Bart van; Bers, Karolien; Ceuninck, W. De; Wagner, Patrick

doi:10.3390/s130709148

Cited by 28 publications

(35 citation statements)

References 31 publications

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“…An additional tuning of the D parameter (not shown) did not significantly improve the noise levels on the signal. These findings are in line with similar PID tuning schemes, performed on the original HTM setup in previous work and indicate that a full PID tuning might not be necessary when future modifications are made to the setup. Furthermore, it implies that the device can be used in various surroundings without significantly affecting its sensitivity.…”

Section: Resultssupporting

confidence: 89%

“…Based on previous results with a similar PID optimization study on the original HTM setup , it was decided to sweep the P and I values between 1 and 12 in the current study with a step width of 1. The average thermal resistance was calculated at 37 °C in de‐ionized water over a period of 30 min for each combination and the standard error ( σ ) was analyzed.…”

Section: Methodsmentioning

confidence: 99%

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Optimization and characterization of a flow cell for heat-transfer-based biosensing

Stilman

Jooken

Wackers

et al. 2017

Phys. Status Solidi A

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In this article, we report on the development of a flow cell optimized for the heat‐transfer method, a versatile biosensing technique. The design of the flow cell ensures that the heat flow is focused with minimal heat loss through the surroundings of the cell. This results in a more stable measuring signal and an improved sensitivity of the measuring technique. The sensor was characterized by performing background measurements in air, water, and phosphate buffered saline (PBS) solution. Heat flow through the setup was simulated using COMSOL in order to provide insight in the contribution of convection to the heat flow and recommendations for possible future improvements to the cell. Additionally, a two‐step algorithm for calculating thermal resistance was defined, allowing the user to accurately derive thermal conductivity from experimental data. Finally, the potential of the flow cell for bacteria (Escherichia coli) detection was assessed and compared with the results obtained in the original HTM setup in a similar experiment. This experiment demonstrates that we were able to improve the limit‐of‐detection (LoD) to 2.10 × 104 colony forming units (CFU) mL−1 by changing the geometry of the measuring cell. Sensor setup for thermal biodetection experiments a directed heat flow.

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Section: Resultssupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Optimization and characterization of a flow cell for heat-transfer-based biosensing

Stilman

Jooken

Wackers

et al. 2017

Phys. Status Solidi A

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“…The parameter of the fit are X = 92.40 and Y = 1.02. The limit of detection can be estimated to be lower than 100 nM, which is in the same order of magnitude as with the setup used in previous measurements without array features [30]. …”

Section: Resultsmentioning

confidence: 75%

Array Formatting of the Heat-Transfer Method (HTM) for the Detection of Small Organic Molecules by Molecularly Imprinted Polymers

Wackers

Vandenryt

Cornelis

et al. 2014

Sensors

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In this work we present the first steps towards a molecularly imprinted polymer (MIP)-based biomimetic sensor array for the detection of small organic molecules via the heat-transfer method (HTM). HTM relies on the change in thermal resistance upon binding of the target molecule to the MIP-type receptor. A flow-through sensor cell was developed, which is segmented into four quadrants with a volume of 2.5 μL each, allowing four measurements to be done simultaneously on a single substrate. Verification measurements were conducted, in which all quadrants received a uniform treatment and all four channels exhibited a similar response. Subsequently, measurements were performed in quadrants, which were functionalized with different MIP particles. Each of these quadrants was exposed to the same buffer solution, spiked with different molecules, according to the MIP under analysis. With the flow cell design we could discriminate between similar small organic molecules and observed no significant cross-selectivity. Therefore, the MIP array sensor platform with HTM as a readout technique, has the potential to become a low-cost analysis tool for bioanalytical applications.

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“…More details regarding the technique and its application to other bioanalytical tasks such as mutation analysis in DNA, cell, and neurotransmitters detection can be found in Refs. .…”

Section: Methodsmentioning

confidence: 99%

“…In view of the limited number of work on phase transitions of SLVs, we propose a novel surface‐sensitive technique, based on monitoring heat transfer through a solid–liquid interface, to detect and monitor phase transitions of adsorbed lipid vesicles. This technique proved successful in detecting single nucleotide polymorphisms in DNA , small organic molecules using molecularly imprinted polymers , and living cells using surface imprinted polymers . Our aim is to assess whether the heat transfer technique can detect SLV phase transitions by measuring R th across a lipid vesicle layer deposited onto a good solid substrate in contact with a liquid environment.…”

Section: Introductionmentioning

confidence: 99%

Phase transitions in lipid vesicles detected by a complementary set of methods: heat‐transfer measurements, adiabatic scanning calorimetry, and dissipation‐mode quartz crystal microbalance

Losada-Pérez

Jiménez-Monroy

Grinsven

et al. 2014

Physica Status Solidi (a)

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We report on the use of the heat transfer method, a novel surface‐sensitive technique based on heat transfer through solid–liquid interfaces, to detect phase transitions of model lipid membranes. We selected the lipid DPPC because of its rich phase behavior in an experimentally accessible temperature range. The vesicles were adsorbed on nanocrystalline diamond films, known as a versatile platform material for biosensing with outstanding heat‐conduction properties. Complementary Peltier‐element‐based adiabatic scanning calorimetry (pASC) and quartz crystal microbalance with dissipation monitoring (QCM‐D) measurements were carried out to monitor the phase transitions in multilamellar and small unilamellar vesicles, respectively. The heat‐transfer measurements revealed reversible jumps upon heating and cooling in the thermal resistance in the vicinity of the expected transition temperature and they agree qualitatively with molecular simulations of the thermal conductivity across a lipid bilayer. The results show the capability of the heat transfer method to detect the main phase transition in DPPC, opening new perspectives for the study of more complex lipid systems and different solid platforms. This work confirms QCM‐D as a useful tool for the assessment of the structural changes upon the phase conversion and shows the capability of pASC to provide high‐resolution thermodynamic information on biophysical systems. Temperature profile of the heat transfer resistance Rth during the main phase transition of a DPPC supported vesicle layer adsorbed on a hydrogen‐terminated nanocrystalline diamond substrate. The arrows indicate the sense of the run: heating (red solid line) and cooling (blue solid line).

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Optimizing the Thermal Read-Out Technique for MIP-Based Biomimetic Sensors: Towards Nanomolar Detection Limits

Cited by 28 publications

References 31 publications

Optimization and characterization of a flow cell for heat-transfer-based biosensing

Optimization and characterization of a flow cell for heat-transfer-based biosensing

Array Formatting of the Heat-Transfer Method (HTM) for the Detection of Small Organic Molecules by Molecularly Imprinted Polymers

Phase transitions in lipid vesicles detected by a complementary set of methods: heat‐transfer measurements, adiabatic scanning calorimetry, and dissipation‐mode quartz crystal microbalance

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