Optimizing the production of transformed pea (Pisum sativum L.) callus using disarmed Agrobacterium tumefaciens strains

Lulsdorf, M.; Rempel, Hans; Jackson, Jennie A.; Baliski, David S.; Hobbs, S. L. A.

doi:10.1007/bf00232100

Cited by 39 publications

(9 citation statements)

References 19 publications

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“…Our results indicate that for a wild-type strain, T-DNA is somewhat more efficiently transferred when located in cis on the Ti plasmid than from a binary vector. Other authors have reported similar findings [25,28], which were attributed to the incomplete stability of the broad-host-range binary plasmids [28]. However, it is also possible that the differences in copy number between the binary vectors and the Ti plasmids are somehow involved.…”

Section: Discussionsupporting

confidence: 58%

Factors affecting the rate of T-DNA transfer from Agrobacterium tumefaciens to Nicotiana glauca plant cells

Mozo

Hooykaas

1992

Plant Mol Biol

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Different factors involved in the early steps of the T-DNA transfer process were studied by using a beta-glucuronidase gene (gusA) as a reporter in Nicotiana glauca leaf disc transformation experiments. The levels of transient expression of the gusA gene in leaf discs infected with several strains or vir mutants correlated well with their virulence phenotype, except for virC mutants. The rate of T-DNA transfer was shown to be stimulated in the case of non-oncogenic strains by the co-transfer of small amounts of oncogenic genes. It was found that the location of the T-DNA in the Agrobacterium genome affected the T-DNA transfer rate especially in virC mutants. The virC mutants transferred the gusA-containing T-DNA located on a binary vector more efficiently than the oncogenic T-DNA of the Ti plasmid. Although wild-type strains induced high levels of gusA expression early after infection, the gusA expression appeared to be lost late after infection in the infected leaf discs. In contrast, in leaf discs infected by virC mutants the level of gusA expression increased steadily in time. A model explaining these results is presented.

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Section: Discussionsupporting

confidence: 58%

Factors affecting the rate of T-DNA transfer from Agrobacterium tumefaciens to Nicotiana glauca plant cells

Mozo

Hooykaas

1992

Plant Mol Biol

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“…The "wild-type" cointegrate orientation in LBG15 permitted higher GUS activity than the binary-construct (LBG17). These findings are consistent with results obtained by McCormick et al (1986), Lulsdorf et al (1991) and Mozo and Hooykaas (1992), but DeKathen and Jacobsen (1990) found no appreciable difference in transformation frequency between cointegrate and binary systems. LBG15 and LBG17 shared virtually identical genetic backgrounds, with the exception of a left and right T-DNA border in pPBI-3002 and a single right border in pPBI-3004.…”

Section: Discussionsupporting

confidence: 94%

Analysis of conditions forAgrobacterium-mediated transformation of tobacco cells in suspension

Rempel

Nelson

1995

Transgenic Research

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We have developed an Agrobacteriurn-mediated transformation system, using tobacco cell suspensions, that permits evaluation of factors affecting transformation within seven days of co-cultivation. Tobacco cell transformation was determined by monitoring fl-glucuronidase (GUS) activity detected in plant cell extracts. The use of a chimeric gene construct, 35S-GUS/INT, containing a portable intron in the uidA reading frame, assured only plant-specific GUS expression. During the co-cultivation period, induction of the bacterial vir-reNon was monitored using a heterologous gene construct composed of a virB promoter fragment from pTiC58 fused to the chloramphenicol acetyltransferase (CAT) gene of Tn9. Tobacco cell transformants were confirmed by antibiotic selection of transformed plant cells and by X-Gluc staining. Maximum transformation was obtained when plant suspension cultures were growing rapidly which also was coincidental with elevated levels of bacterial vir-region expression. One week after co-cultivation, the transformed cultures exhibited a stable pattern of GUS activity which remained constant without antibiotic selection. The system was used to compare the virulence of a number of Agrobacterium strains. GUS activity of plant cells co-cultivated with a strain containing a cointegrate plasmid was 3-fold higher than that of one with a binary configuration of the T-DNA. When the co-cultivating Agrobacterium strain also carried the plasmid used to monitor vir induction, the frequency of transformation was reduced by as much as 97%.

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“…Particularly, cocultivation additives were not studied systematically (only sporadic data on acetosyringone use are available-De Kathen and Jacobsen 1990; Lulsdorf et al 1991;Grant et al 1995;Nadolska-Orczyk and Orczyk 2000;Svabova et al 2005. Krejc{ et al (2007) also reported Agrobacterium-mediated gene transfer in pea.…”

Section: Peamentioning

confidence: 94%

Gene Transfer in Legumes

Atif¹,

Patat-Ochatt²,

Švábová

et al. 2012

Progress in Botany

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Optimizing the production of transformed pea (Pisum sativum L.) callus using disarmed Agrobacterium tumefaciens strains

Cited by 39 publications

References 19 publications

Factors affecting the rate of T-DNA transfer from Agrobacterium tumefaciens to Nicotiana glauca plant cells

Factors affecting the rate of T-DNA transfer from Agrobacterium tumefaciens to Nicotiana glauca plant cells

Analysis of conditions forAgrobacterium-mediated transformation of tobacco cells in suspension

Gene Transfer in Legumes

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