Optimizing the malathion degrading potential of a newly isolated Bacillus sp. AGM5 based on Taguchi design of experiment and elucidation of degradation pathway

Dar, Mohd Ashraf; Kaushik, Garima

doi:10.1007/s10532-022-09986-x

Cited by 7 publications

(8 citation statements)

References 73 publications

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“…Micrococcus aloeverae (MAGK3), Bacillus cereus (AGB3), and Bacillus paramycoides (AGM5) capable of degrading malathion were isolated from agricultural soils located in Ajmer district in Rajasthan, state of India, from our previous studies [15,16]; they degraded (50-1000 mgL −1 ) malathion in liquid cultures. The current study examined the biodegradation capacity of these bacterial strains and their consortia in the soil.…”

Section: Microorganismsmentioning

confidence: 99%

“…The isolates were identified via their morphological and biochemical properties, and were further confirmed based on 16S rRNA gene sequencing. As stated by Dar and Kaushik [15], PCR amplification and sequencing of 16S rDNA genes were carried out, and the sequence similarity was assessed on 17 May 2021 via the BLAST function in NCBI Genbank (https://www.ncbi.nlm.nih.gov).…”

Section: Identification Of Malathion-degrading Bacteriamentioning

confidence: 99%

“…Thus, the presence of OPPs like malathion in the environment is causing concern worldwide due to its impact on ChE activity and the nervous system, and is considered a main hazard to public health [4]. Malathion is categorized as carcinogenic, both to humans and animals, and high exposure levels can disrupt nerve cells and cause neurotoxicity in animals, as well as impair higher vertebrate immunity [15]. Due to its effects on the adrenal glands and central nervous systems of higher vertebrates and invertebrates, malathion is highly toxic to aquatic and terrestrial animals [16].…”

Section: Introductionmentioning

confidence: 99%

“…The biodegradation of OPPs is thought to include a variety of bacterial species. We have identified and assessed the malathion degradation ability of bacterial species in liquid culture in some of our earlier publications [6,15]. The bacterial strains that caused higher malathion degradation were labeled as effective malathion degraders, and were identified as Micrococcus aloeverae MAGK3 (GenBank acc # MZ220366), Bacillus cereus AGB3 (GenBank acc # ON150776), and Bacillus paramycoides AGM5 (GenBank acc # OM021874) by the National Center for Biotechnology Information (NCBI) after taxonomic characterization.…”

Section: Introductionmentioning

confidence: 99%

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Biodegradation of Malathion in Amended Soil by Indigenous Novel Bacterial Consortia and Analysis of Degradation Pathway

Dar,

Kaushik

2023

Soil Systems

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The capabilities of pure bacterial strains and their consortia isolated from agricultural soil were evaluated during a bioremediation process of the organophosphate pesticide malathion. The pure bacterial strains efficiently degraded 50.16–68.47% of the pesticide within 15 days of incubation, and metabolites were observed to accumulate in the soil. The consortia of three bacterial species [Micrococcus aloeverae (MAGK3) + Bacillus cereus (AGB3) + Bacillus paramycoides (AGM5)] degraded the malathion more effectively, and complete malathion removal was observed by the 15th day in soils inoculated with that consortium. In contrast, the combined activity of any two of these strains was lower than the mixed consortium of all of the strains. Individual mixed consortia of Micrococcus aloeverae (MAGK3) + Bacillus cereus (AGB3); Micrococcus aloeverae (MAGK3) + Bacillus paramycoides (AGM5); and Bacillus cereus (AGB3) + Bacillus paramycoides (AGM5) caused 76.58%, 70.95%, and 88.61% malathion degradation in soil, respectively. Several intermediate metabolites like malaoxon, malathion monocarboxylic acid, diethyl fumarate, and trimethyl thiophosphate were found to accumulate and be successively degraded during the bioremediation process via GC–MS detection. Thus, inoculating with a highly potent bacterial consortium isolated from in situ soil may result in the most effective pesticide degradation to significantly relieve soils from pesticide residues, and could be considered a prospective approach for the degradation and detoxification of environments contaminated with malathion and other organophosphate pesticides. This study reports the use of a mixed culture of Indigenous bacterial species for successful malathion degradation.

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Section: Microorganismsmentioning

confidence: 99%

Section: Identification Of Malathion-degrading Bacteriamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biodegradation of Malathion in Amended Soil by Indigenous Novel Bacterial Consortia and Analysis of Degradation Pathway

Dar,

Kaushik

2023

Soil Systems

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“…The term -pesticide‖ is defined by the World Health Organization (WHO) as any chemical substance intended to kill pests (weeds, rodents, insects, and fungus) [3]. Due to the excessive or improper use of pesticides, the rising demand for food production worldwide has produced a significant decline in food quality and severe environmental effects [4,5], but it has also had an adverse impact on human health. More than 20% of pesticides are estimated to reach non-target species and air, water, and soil.…”

Section: Introductionmentioning

confidence: 99%

Kinetic and Equilibrium Isotherm of Pesticides (Atrazine) Adsorption Unto Activated Carbon of Calabash (Lagenaria Siceraria)

Chukwudi-Madu

Agboeze

Chime

et al. 2022

EPRA

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The adsorption of pesticides (Atrazine) from water onto activated carbon prepared from calabash was investigated. The kinetic and equilibrium study was carried out, and data obtained showed that time duration, the concentration of the solution, the dosage of activated carbon and the pH of the solution greatly influenced the adsorption process. The adsorption kinetic study of the pesticide was done using various models. The result best fits pseudo-second-order using the coefficient regression value (0.9922) and intraparticle diffusion. Equilibrium isotherm data were analysed using the Langmuir isotherm model and Freundlich isotherm model. The result of the regression value provided Freundlich isotherm as the best fit to the experimental data, indicating the presence of heterogeneous layer sorption from the regression value of 0.7294. This study suggested that the activated carbon of calabash as a low-cost material is suitable for the adsorption of pesticides from water. Hence it could be applied as a cheap and effective material in removing pesticides in water bodies or industrial waste before disposal. KEYWORDS: Pesticides, activated carbon, adsorption capacity, isotherm, kinetic study, calabash.

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Bacillus-Mediated Degradation of Recalcitrant Agricultural Pesticides: A Cutting-Edge Approach Towards the Clean-Up of Environmental Contaminants

Shahid,

Singh,

Ilyas

et al. 2024

Microorganisms for Sustainability

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Optimizing the malathion degrading potential of a newly isolated Bacillus sp. AGM5 based on Taguchi design of experiment and elucidation of degradation pathway

Cited by 7 publications

References 73 publications

Biodegradation of Malathion in Amended Soil by Indigenous Novel Bacterial Consortia and Analysis of Degradation Pathway

Biodegradation of Malathion in Amended Soil by Indigenous Novel Bacterial Consortia and Analysis of Degradation Pathway

Kinetic and Equilibrium Isotherm of Pesticides (Atrazine) Adsorption Unto Activated Carbon of Calabash (Lagenaria Siceraria)

Bacillus-Mediated Degradation of Recalcitrant Agricultural Pesticides: A Cutting-Edge Approach Towards the Clean-Up of Environmental Contaminants

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