Optimizing the formation of biocompatible gold nanorods for cancer research: Functionalization, stabilization and purification

Bogliotti, Nicolas; Oberleitner, Birgit; Di-Cicco, Aurélie; Florent, Jean‐Claude; Semetey, Vincent

doi:10.1016/j.jcis.2011.01.053

Cited by 30 publications

(26 citation statements)

References 41 publications

(80 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, usually after a month of storage in the colloidal solution, the POSAB GNRs lost stability and aggregated as observed by a solution color change from blue to purple. Despite the stability of GNRs increasing with the length of the polyethylene glycol (PEG) chain (note the threshold of PEG molecular weight for the efficient steric nanoparticle stabilization was determined ≥2000 ), even the short hexaethylene glycol chain bearing the quaternary ammonium cation allowed for the effective ligand exchange and stabilization of the GNRs in the water via the electrostatic double layer. Although the storage stability of POSAB GNRs was less than a month compared to several months stability of MTAB GNRs , it was still acceptable.…”

Section: Resultsmentioning

confidence: 99%

Highly hydrophilic cationic gold nanorods stabilized by novel quaternary ammonium surfactant with negligible cytotoxicity

Salajkova

Šrámek

Maliňák

et al. 2019

Journal of Biophotonics

View full text Add to dashboard Cite

The photothermal cancer therapy using cationic gold nanorods (GNRs) stabilized by quaternary ammonium salts (QAS) have a great potential to enhance conventional cancer treatment as it promises the effective eradication of cancer cells including cells resistant to radio‐ and chemo‐therapy and the stimulation of anti‐tumor immune response. However, as the cytotoxicity of the conventional alkanethiol‐QAS compounds limits their utility in medicine, here we developed GNRs modified by novel highly hydrophilic cationic surfactant composed of the quaternary ammonium group and ethylene glycol chain N,N,N‐trimethyl‐3,6,9,12,15‐pentaoxaheptadecyl‐17‐sulfanyl‐1‐ammonium bromide (POSAB) showing insignificant cytotoxicity in the free state. Surface modification of GNRs by POSAB allowed to prepare nanoparticles with good stability in water, high cellular uptake and localization in lysosomes that are a promising alternative to alkanethiol‐stabilized GNRs especially for biomedical applications.

show abstract

Section: Resultsmentioning

confidence: 99%

Highly hydrophilic cationic gold nanorods stabilized by novel quaternary ammonium surfactant with negligible cytotoxicity

Salajkova

Šrámek

Maliňák

et al. 2019

Journal of Biophotonics

View full text Add to dashboard Cite

show abstract

“…The surface plasmon absorption of the prepared GNRs has two bands at 800 nm and 514 nm which were attributed to the longitudinal and the transverse electronic oscillation, respectively. Apart from the shape, size and aspect ratio of GNRs which influence their photoplasmonic properties [23]; the degree of PEGylation and chain length of PEG affect the biocompatibility of GNRs like other nanomaterials [24]. Accordingly, the current formulation of GNRs comprises unique and tunable nanostructure with great potentiality in photoplasmonic therapy of solid tumors.…”

Section: Discussionmentioning

confidence: 99%

Tissue Distribution and Efficacy of Gold Nanorods Coupled with Laser Induced Photoplasmonic Therapy in Ehrlich Carcinoma Solid Tumor Model

et al. 2013

View full text Add to dashboard Cite

Gold nanorods (GNR) within tumor microregions are characterized by their ability to absorb near IR light and emit heat in what is called photoplasmonic effect. Yet, the efficacy of nanoparticles is limited due to intratumoral tissue distribution reasons. In addition, distribution of GNRs to normal tissue might result in non specific toxicity. In the current study, we are assessing the intratumoral and tissue distribution of PEGylated GNRs on the top of its antitumor characteristics when given intravenously or intratumoral to solid tumor bearing mice and coupled with laser photoplasmonic sessions. PEGylated GNRs with a longitudinal size of less than 100 nm were prepared with aspect ratio of 4.6 showing strong surface plasmon absorption at wavelength 800 nm. Pharmacokinetics of GNR after single I.V. administration (0.1 mg/kg) showed very short systemic circulating time (less than 3 h). On the other hand, tissue distribution of I.V. GNR (0.1 mg/kg) to normal animals showed preferential deposition in spleen tissue. Repeated administration of I.V. GNR resulted in preferential accumulation in both liver and spleen tissues. In addition, I.V. administration of GNR to Ehrlich carcinoma tumor bearing mice resulted in similar tissue distribution; tumor accumulation and anti-tumor effect compared to intratumoral administration. In conclusion, the concentration of GNR achieved within tumors microregions after I.V. administration was comparable to I.T. administration and sufficient to elicit tumoral growth arrest when coupled with laser-aided photoplasmonic treatment.

show abstract

“…Over the past decade, gold nanoparticles (Au NPs) have attracted considerable interest for biological applications such as labeling (Seekell et al, 2012), drug or gene delivery (Xu et al, 2012), heating (Cheng et al, 2009), sensing (Truong et al, 2012), and imaging (Yang et al, 2012;Zhan et al, 2010). These nanometer-scale metallic particles are attractive because they can be synthesized in large quantities, support a wide range of surface functionalizations that make them chemically stable (Bogliotti et al, 2011;Hu et al, 2006;Seekell et al, 2012) and offer unusual optical properties. The presence of nanoparticles located intracellularly or in contact with biological tissue and fluids also enables the exploitation of a variety of specific photonic properties.…”

Section: Introductionmentioning

confidence: 99%

Laser exposure of gold nanorods can increase neuronal cell outgrowth

Paviolo

Haycock

Yong

et al. 2013

Biotech & Bioengineering

100

View full text Add to dashboard Cite

The usage of gold nanoparticles (Au NPs) in biological applications has risen significantly over the last 10 years. With the wide variety of chemical and biological functionalization available and their distinctive optical properties, Au NPs are currently used in a range of biological applications including sensing, labeling, drug delivery, and imaging applications. Among the available particles, gold nanorods (Au NRs) are particularly useful because their optical absorption can be tuned across the visible to near infrared region. Here, we present a novel application of Au NRs associated with low power laser exposure of NG108-15 neuronal cells. When cells were irradiated with a 780 nm laser, the average number of neurons with neurites increased. A similar stimulatory effect was observed for cells that were cultured with poly-(4-styrenesulfonic acid)-coated and silica-coated Au NRs. Furthermore, when the NG108-15 cells were cultured with both bare and coated Au NRs and then irradiated with 1.2-7.5 W/cm(2) at 780 nm, they showed a neurite length increase of up to 25 µm versus control. To the best of our knowledge, this effect has never been reported before. While the pathways of the stimulation is not yet clear, the data presented here demonstrates that it is linked to the absorption of light by the Au NRs. These initial results open up new opportunities for peripheral nerve regeneration treatments and for novel approaches to addressing central nervous system axons following spinal cord injury.

show abstract

Optimizing the formation of biocompatible gold nanorods for cancer research: Functionalization, stabilization and purification

Cited by 30 publications

References 41 publications

Highly hydrophilic cationic gold nanorods stabilized by novel quaternary ammonium surfactant with negligible cytotoxicity

Highly hydrophilic cationic gold nanorods stabilized by novel quaternary ammonium surfactant with negligible cytotoxicity

Tissue Distribution and Efficacy of Gold Nanorods Coupled with Laser Induced Photoplasmonic Therapy in Ehrlich Carcinoma Solid Tumor Model

Laser exposure of gold nanorods can increase neuronal cell outgrowth

Contact Info

Product

Resources

About