Optimizing Sterilization Condition for the Initiation of Sweet Potato (Ipomoea batatas) Culture in vitro

Hammond, Ronald B.; Buah, J. N.; Asare, Paul A.; Acheampong, Susanna

doi:10.3923/ajbkr.2014.25.37

Cited by 8 publications

(17 citation statements)

References 3 publications

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“…Indeed, the results of Figs. 2 and 3 have shown that infection and necrosis are the main problems whose control is indispensable for establishment of to in vitro culture as demonstrated by Ahanhanzo et al(2008) on yam and Hammond et al (2014) on sweet potato. These observations revealed that the explants were infected with fungi and bacteria.…”

Section: Discussionmentioning

confidence: 99%

“…These observations revealed that the explants were infected with fungi and bacteria. Omamor et al (2007) and Hammond et al (2014) have shown that the contamination by fungi and bacteria can compromise in vitro culture. The results showed that, more the concentration of mercuric chloride (0.05%, 0.1%, 0.5% and 1%), more the decrease of average infection and more the increase of average necrosis in all the three varieties.…”

Section: Discussionmentioning

confidence: 99%

“…One per cent (1%) concentration was effective for explant disinfection for 10 min immersion, however, it induced a high rate of necrosis. The work of Hammond et al (2014) showed that the concentrations of 0.4% and 0.6% mercuric chloride for the same duration were not effective to control the infections. The concentration of 1% HgCl 2 was considered effective for the disinfection of sweet potatoes explants according to the same author.…”

Section: Discussionmentioning

confidence: 99%

“…This difference of survival average between the varieties may be genotypic origin. Hammond et al (2014) and Doukouré (2000) also used 1% mercuric chloride to have high survival rates in several sweet potato and yam genotypes respectively. It is important to note, however, that most authors prefer to use sodium hypochlorite which is less toxic to the explants (Sivparsad and Gubba, 2012 on sweet potato).…”

Section: Discussionmentioning

confidence: 99%

“…The success of in vitro culture therefore requires adequate disinfection of the explant using chemicals such as sodium hypochlorite, mercury lauryl and mercuric chloride. The use of this last proved more effective for the disinfection of the woody species like teak, herbaceous plants such as yams (Ahanhanzo et al, 2008 and and sweet potatoes (Hammond et al, 2014). Thus, the development of an effective disinfection protocol will, in a long term, reduce the cost of producing plantlets for better accessibility to producers.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Influence of Mercuric Chloride on Survival and Suitability for In Vitro Regeneration of Three Sweet Potato Landraces (Ipomoea batatas L.) Produced in Benin

Doussoh¹,

Dangou-Sossou²,

Houédjissin³

et al. 2017

Int. J. Curr. Res. Biosci. Plant Biol.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Influence of Mercuric Chloride on Survival and Suitability for In Vitro Regeneration of Three Sweet Potato Landraces (Ipomoea batatas L.) Produced in Benin

Doussoh¹,

Dangou-Sossou²,

Houédjissin³

et al. 2017

Int. J. Curr. Res. Biosci. Plant Biol.

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Development of control strategies for bacteria and fungi associated with a micropropagated new cultivar of orange-fleshed sweet potato (Ipomoea batatas cv. Agrosavia–Aurora)

Pérez-Pazos

Rosero

Cardinale

et al. 2023

Hortic. Environ. Biotechnol.

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Vegetative propagation of sweet potato lead to the accumulation of diseases from generation to generation, which represents a threat to both productivity and conservation of genetic resources. In vitro techniques can help to overcome phytosanitary problems by applying plant material cleaning strategies. The objective of this study was to develop in vitro micropropagation strategies for the production of high-quality plant material of an orange-fleshed variety of sweet potato recently released in Colombia. Molecular identification of contaminating microorganisms was performed by sequencing the 16S rRNA gene for bacteria and ITS for fungi. Five disinfection protocols were evaluated, three of which were previously developed for sweet potato and included disinfection with 0.5, 1, and 2% sodium hypochlorite respectively, while two protocols are proposed in this work and included washing with povidone-iodine, disinfection with sodium hypochlorite 2%; one of these two new protocols also contains acetic acid and quaternary ammonium. For the evaluation of the viability of in vitro plants after disinfection, they were acclimatized in a greenhouse, reintroduced, and a molecular testing by PCR of 16S rRNA gene and ITS was carried out to verify the phytosanitary status of the material. The contaminating microorganisms found were filamentous fungi of the genera Fusarium, Sarocladium, Cladosporium and Aspergillus, yeasts of the genera Pseudozyma and Moesziomyces, and the actinobacterium Curtobacterium sp. The results indicated that washing with povidone-iodine and disinfection with 2% sodium hypochlorite, acetic acid and quaternary ammonium was the most efficient disinfection protocol, reducing the number of contaminated cultures by up to 10% and eradicating 70% of contaminants. The in vitro plants established in the greenhouse remained healthy and, after reintroduction, the molecular test for bacteria and fungi was negative. These results allowed the generation of an optimized protocol that can be incorporated into the in vitro micropropagation process to generate contamination-free sweet potato seeds.

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In Vitro Regeneration of Stevia (Stevia rebaudiana Bertoni) and Evaluation of the Impacts of Growth Media Nutrients on the Biosynthesis of Steviol Glycosides (SGs)

et al. 2022

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A plant tissue culture protocol from stevia was optimized for the production of planting materials and the natural sweetener, rebaudioside A. The highest survivability (88.90% ± 5.55) of explants was achieved at 15 and 30 days after culture initiation (DACI) on Murashige and Skoog (MS) media by sterilization with 30% Clorox (5 min) and 10% Clorox (10 min), respectively. Supplementation of MS with 0.50 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D) and 0.10 mg/L zeatin produced 50% callus at 15 DACI while 1.50 mg/L 2,4-D and 0.10 mg/L zeatin at 30 DACI increased callus production to 76.67%. The highest shoot proliferation per callus was achieved with 10.00 mg/L 6-benzyl amino purine (BAP) in MS at 15 DACI (5.80) and 30 DACI (12.33). The longest shoots of 4.31 cm and 6.04 cm at 15 and 30 DACI, respectively, were produced using BAP (10.00 mg/L) and 1.00 mg/L naphthalene acetic acid (NAA). MS media (0.50 strength) induced 2.86 and 6.20 roots per shoot and produced 3.25 cm and 7.82 cm long roots at 15 and 30 DACI, respectively. Stevia grown on 0.25 MS accumulated the highest concentration of rebaudioside A (6.53%), which correlated with the expression level of its biosynthetic gene uridine-diphosphate-dependent (UDP)-glycosyltransferase (UGT76G1).

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Optimizing Sterilization Condition for the Initiation of Sweet Potato (Ipomoea batatas) Culture in vitro

Cited by 8 publications

References 3 publications

Influence of Mercuric Chloride on Survival and Suitability for In Vitro Regeneration of Three Sweet Potato Landraces (Ipomoea batatas L.) Produced in Benin

Influence of Mercuric Chloride on Survival and Suitability for In Vitro Regeneration of Three Sweet Potato Landraces (Ipomoea batatas L.) Produced in Benin

Development of control strategies for bacteria and fungi associated with a micropropagated new cultivar of orange-fleshed sweet potato (Ipomoea batatas cv. Agrosavia–Aurora)

In Vitro Regeneration of Stevia (Stevia rebaudiana Bertoni) and Evaluation of the Impacts of Growth Media Nutrients on the Biosynthesis of Steviol Glycosides (SGs)

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