Optimizing proliferation and characterization of multipotent stem cells from porcine adipose tissue

Wang, Kai-Hung; Kao, An-Pei; Wangchen, Hsuan; Wang, Fuyuan; Chang, Chih‐Hau; Chang, Chia‐Cheng; Lin, Sin-Daw

doi:10.1042/ba20070201

Cited by 31 publications

(30 citation statements)

References 29 publications

(44 reference statements)

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“…The gap junctional intercellular communication was studied by the scrape loading-dye transfer technique (34,38).…”

Section: Gap Junctional Intercellular Communicationmentioning

confidence: 99%

“…These endometrial MSCs were tested for multilineage differentiation potential, that is, adipogenesis, osteogenesis, chondrogenesis, cardiogenesis, and neurogenesis, following previously reported protocols (30,31,34,(39)(40)(41). Cells first were subcultured and incubated in D-medium (42) with 10% fetal bovine serum.…”

Section: Multilineage Differentiationmentioning

confidence: 99%

“…Besides the origin of these stem-progenitor cells from the uterine tissue, other sources have been proposed (27,28). Mesenchymal stem cells (MSCs) have been identified in various tissues (29)(30)(31)(32)(33)(34). The MSCs in endometrium were believed to be responsible for the remarkable regenerative ability of endometrial cells (35)(36)(37).…”

mentioning

confidence: 99%

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Comparative study of human eutopic and ectopic endometrial mesenchymal stem cells and the development of an in vivo endometriotic invasion model

Kao

Wang

Chang

et al. 2011

Fertility and Sterility

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128

100

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“…The gap junctional intercellular communication was studied by the scrape loading-dye transfer technique (34,38).…”

Section: Gap Junctional Intercellular Communicationmentioning

confidence: 99%

Section: Multilineage Differentiationmentioning

confidence: 99%

See 1 more Smart Citation

Comparative study of human eutopic and ectopic endometrial mesenchymal stem cells and the development of an in vivo endometriotic invasion model

Kao

Wang

Chang

et al. 2011

Fertility and Sterility

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128

100

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“…Such cells showed potential to differentiate into different mesodermal lineages, such as bone, cartilage, fat, muscle, and cardiomyocytes, and expressed different SC markers, showing similarities with human adipose tissue-derived MSCs. [34][35][36][37][38] Interestingly, pigs are already widely used in this field regarding endovascular device implantation, such as stents. 62,63 However, it is a good preclinical model to test the effect of SCs specifically in the area of SCs applied to cardiovascular diseases.…”

Section: Swine Adipose Tissue Scsmentioning

confidence: 99%

“…Therefore, these cells can be used in several areas, such as in regenerative therapy. 7 Currently, cells with characteristics similar to those of human MSCs were isolated in vitro from several animals (pigs, [34][35][36][37][38] dogs, 39 sheep 40 ). These animals are often used in regenerative medicine research as animal models for osteoarticular diseases, 41 spinal cord injury, 42 and myocardial infarction.…”

Section: Adipose Tissue Mscsmentioning

confidence: 99%

Células-tronco de tecido adiposo e a importância da padronização de um modelo animal para experimentação pré-clínica

Zuttion¹,

Wenceslau²,

Lemos³

et al. 2013

Rev. Bras. Cardiol. Invasiva

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Stem cells are undifferentiated cells and can self-renew and differentiate into various cell types, besides having immunomodulating properties and paracrine effects in response to tissue injury, and may therefore treat injuries and diseases or even replace damaged or lost cells. Adipose tissue is an attractive source of adult stem cells, since the human body has a large reserve that is obtained in large amounts by minimally invasive methods. Interest in these cells has been increasing steadily due to their properties and possible applications in regenerative medicine and cell therapy. A large part of these investigations are focused on cardiovascular diseases, which are a leading cause of morbidity and mortality worldwide. Although in recent years treatments have advanced in cardiology, the development of new therapies to recover the damaged tissue still remains one of the main goals of cardiac research. However, to achieve effective results, in vivo and in vitro animal models for preclinical studies and consequently for application in humans must be standardized. The development of preclinical models in large animals requires the use of well-characterized animal cell lines, similar to human cells, and the use of the porcine model represents a great advantage for preclinical translational research. DESCRIPTORS: Original Article RESUMO Células-Tronco de Tecido Adiposo e a Importância da Padronização de um Modelo Animal para Experimentação Pré-ClínicaAs células-tronco são células indiferenciadas, capazes de se autorrenovar e de se diferenciarem em diversos tipos celulares, além de apresentarem propriedades imunomoduladoras e efeitos parácrinos mediante injúria tecidual, podendo, dessa forma, tratar lesões e doenças ou ainda substituir células danificadas ou perdidas. Dentre as fontes de células-tronco adultas, o tecido adiposo é uma fonte atrativa, pois o organismo humano possui grande reserva desse tecido, que, por sua vez, é obtido em grandes quantidades por meio de métodos pouco invasivos. O interesse nessas células vem aumentando constantemente devido a suas propriedades e possíveis aplicações na medicina regenerativa e terapia celular. Grande parte dessas pesquisas está voltada para doenças cardiovasculares, que são a principal causa de morbidade e mortalidade em todo o mundo. Embora nos últimos anos, os tratamentos em cardiologia tenham avançado, o desenvolvimento de novas terapias que recuperem o tecido danificado ainda permanece como um dos objetivos principais das pesquisas cardíacas. Porém, para obter resultados eficazes, é necessária a padronização de modelos animais in vivo e in vitro para estudos pré-clínicos e, consequentemente, a aplicação em humanos. O desenvolvimento de modelos pré-clínicos em animais de grande porte exige o uso bem caracterizado de linhagens de células animais semelhantes aos seus equivalentes humanos. O modelo suíno representa uma grande vantagem para a investigação translacional pré-clínica.

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Upregulation of Nanog and Sox‐2 genes following ectopic expression of Oct‐4 in amniotic fluid mesenchymal stem cells

Wang

Kao²,

Chang

et al. 2015

Biotech and App Biochem

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Octamer-binding transcription factor 4 (Oct-4), an important gene regulating stem cell pluripotency, is well-known for its ability to reprogram somatic cells in vitro, either alone or in concert with other factors. The aim of this study was to assess the effect of ectopic expression of Oct human amniotic fluid stem cells. We developed a novel method for isolation of putative human amniotic fluid-derived multipotent stem cells. These cells showing mesenchymal stem cell phenotypes (human amniotic fluid-derived mesenchymal stem cells, hAFMSCs) were transfected with a plasmid carrying genes for Oct-4 and the green fluorescent protein (GFP). The stably transfected cells, hAFMSCs-Oct4/GFP, were selected by using G418 and found to express the GFP reporter gene under the control of Oct-4 promoter. We found that hAFMSCs developed by our method possess very high self-renewal ability (about 78 cumulative population doublings) and multilineage differentiation potency. Significantly, the hAFMSCs-Oct4/GFP cells showed enhanced expression of the three major pluripotency genes Oct-4, Nanog, and Sox-2, and increased colony-forming ability and growth rate compared with the parental hAFMSCs. We demonstrated that the ectopic expression of Oct-4 gene in hAFMSCs with high self-renewal ability could upregulate Nanog and Sox-2 gene expression and enhance cell growth rate and colony-forming efficiency. Therefore, the ectopic expression of Oct-4 could be a strategy to develop pluripotency in hAFMSCs for clinical applications.

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Optimizing proliferation and characterization of multipotent stem cells from porcine adipose tissue

Cited by 31 publications

References 29 publications

Comparative study of human eutopic and ectopic endometrial mesenchymal stem cells and the development of an in vivo endometriotic invasion model

Comparative study of human eutopic and ectopic endometrial mesenchymal stem cells and the development of an in vivo endometriotic invasion model

Células-tronco de tecido adiposo e a importância da padronização de um modelo animal para experimentação pré-clínica

Upregulation of Nanog and Sox‐2 genes following ectopic expression of Oct‐4 in amniotic fluid mesenchymal stem cells

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