Optimizing production of polyunsaturated fatty acids in Marchantia polymorpha cell suspension culture

Blue light enhances the accumulation of eicosapentaenoic acid in a liverwort, Marchantia polymorpha L.

“…To develop an economical plant factory as a new source of these valuable PUFAs, it is important to achieve increased productivity. Also, the optimization of light conditions is needed to enhance the accumulation of AA and EPA in liverwort thallus, as was reported in the study of cultured liverwort cells (Chiou et al 2001). Here, we report the effect of blue light on EPA accumulation in liverwort thallus and discuss efficient light conditions for PUFA accumulation.…”

“…Chiou et al (2001) found that intracellular lipid content of liverwort cultured cells among various light intensities from 3 to 32 mmol m Ϫ2 s Ϫ1 was highest at 20 mmol m Ϫ2 s…”

“…Approximately 10% of the total fatty acids contained in the thallus and cultured cells of liverwort, Marchantia polymorpha, are AA and EPA (Kajikawa et al 2008, Shinmen et al 1991. Chiou et al (2001) examined the optimization of production of PUFAs using cultured liverwort cells and reported that PUFA production was strongly associated with cell growth. This suggests that the accumulation of AA and EPA was promoted by the optimization of growth conditions, including light, temperature and nutrition.…”

Improvement of arachidonic acid and eicosapentaenoic acid production by increasing the copy number of the genes encoding fatty acid desaturase and elongase into Pichia pastoris

“…ARA and EPA in Ph. tricornutum can accumulate up to 30% of total fatty acids (Domergue et al 2002), whereas their content is only about 11% of total fatty acids in M. polymorpha (Chiou et al 2001). This indicates that the genes encoding desaturases and elongases from organisms which have high long-chain PUFAs contents may be suitable for producing desirable PUFAs by genetic manipulation.…”

Cold-Induced Accumulation of ω-3 Polyunsaturated Fatty Acid in a Liverwort,Marchantia polymorphaL.

“…[9][10][11][12][13][14] Recently, Chodok et al 14) examined several growth conditions that have an effect on the production of LCPUFAs using cell cultures of Physcomitrella patens, which also accumulates AA and EPA. They reported that growth temperature significantly affected LCPUFA production, and that the proportion of !-3 to !-6 fatty acids was highest at a lower temperature (10 C).…”