Optimizing pharmacotherapy of systemic lupus erythematosus: the pharmacist role

Hussaini, Maryam Al; Hammouda, Emad I.; Hammouda, Ahmed E.

doi:10.1007/s11096-014-9966-1

Cited by 7 publications

(3 citation statements)

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“…Treatment of SLE patients is generally individualized, and the mainstay depends on limiting clinical symptoms and decreasing the risk of permanent damage to organs [Afzal et al, 2014;Al et al, 2014]. 1,25-dihydroxyvitamin D3 is known to affect immune responses [Alvarez-Rodriguez et al, 2012], and its reduced serum levels have been reported in SLE patients, especially in the active phase of the disease [Mora et al, 2008].…”

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confidence: 99%

Vitamin D3 Alters the Expression of Toll‐like Receptors in Peripheral Blood Mononuclear Cells of Patients With Systemic Lupus Erythematosus

Yazdanpanah

Mahmoudi

Sahebari

et al. 2017

J of Cellular Biochemistry

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Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by production of inflammatory cytokines and autoreactive antibodies due to the loss of immune tolerance. Recognition of self-nucleic acids by intracellular Toll-like receptors (TLRs) can overactivate immune responses and this abnormal activation of TLRs contributes to the pathogenesis of the disease. In recent years, anti-inflammatory and immunomodulatory effects of 1,25-dihydroxyvitamin D3 (VitD3) on the immune system has received particular attention. The present study investigated the effects of vitamin D3 on the expression of TLR3, TLR7, and TLR9 in SLE patients. Study participants included 20 SLE patients and 20 age- and sex-matched healthy controls. Peripheral blood mononuclear cells (PBMCs) were isolated and cultured in the presence or absence of vitamin D3 (50 nM). Then RNA was extracted, cDNA was synthesized and gene expression levels of TLR3, TLR7, and TLR9 were assessed using real-time PCR. Up-regulated expression levels of TLR7 and TLR9 were observed in the PBMCs of SLE patients in comparison with controls. Culturing PBMCs with vitamin D3 significantly down-regulated the expression of TLR3 (8.86 ± 4.2 for SLE patients vs. 45.34 ± 18.6 for control; P = 0.03), TLR7 (17.91 ± 7.7 for SLE patients vs. 242.37 ± 89.6 for controls; P = 0.0001) and TLR9 (4.67 ± 1.9 for SLE patients vs. 8.9 ± 1.5 for controls; P = 0.007) in SLE patients in comparison with healthy controls. The results of the current study suggest that vitamin D3 could exert some of its immunomodulatory effects in SLE patients via affecting the expression levels of some TLRs. J. Cell. Biochem. 118: 4831-4835, 2017. © 2017 Wiley Periodicals, Inc.

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confidence: 99%

Vitamin D3 Alters the Expression of Toll‐like Receptors in Peripheral Blood Mononuclear Cells of Patients With Systemic Lupus Erythematosus

Yazdanpanah

Mahmoudi

Sahebari

et al. 2017

J of Cellular Biochemistry

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“…ephedrine, arylalkene) [16,17,18]. Propiophenone derivatives called propafenone are primarily known on their antiarrhythmic action, but they are also involved in treatment of many different diseases including lupus erythematosus, epilepsy, Alzheimer's disease, malaria, ebola, cancer [19][20][21][22][23][24][25]. In addition, recent studies have shown that analogs of propafenone exhibit antifungal activity [26].…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effect of propafenone derivatives on pseudomonas aeruginosa biofilm and pyocyanin production

et al. 2020

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Introduction/Objective Biofilm and pyocyanin production are essential components of Pseudomonas aeruginosa virulence and antibiotic resistance. Our objective was to examine inhibitory effect of synthetized propafenone derivatives 3-(2-Fluorophenyl)-1-(2-(2-hydroxy-3-propylamino-propoxy)-phenyl)-propan-1-one hydrochloride (5OF) and3-(2-Trifluoromethyl-phenyl)-1-(2-(2-hydroxy-3-propylamino-propoxy)-phenyl)-propan-1-one hydrochloride (5CF3) on biofilm and pyocyanin in Pseudomonas aeruginosa clinical strains. Methods Effects were tested on nine clinical isolates and one control laboratory strain of P. aeruginosa. In vitro analysis of biofilm growing was performed by incubating bacteria (0.5 McFarland) with 5OF and 5CF3 (500-31.2 µg/ml) and measuring optical density (OD) at 570 nm. Bacteria in medium without compounds were positive control. Blank medium (an uninoculated medium without test compounds) was used as negative control. Pyocyanin production was estimated by OD at 520 nm, after bacteria incubated with 5CF3 and 5OF (250 and 500 μg/ml), treated with chloroform, and chloroform layer mixed with HCl. Results A total of 500 µg/ml of 5OF and 5CF3 completely inhibited biofilm formation in 10/10 and 4/10 strains, respectively. A total of 250 µg/ml of 5OF and 5CF3 strongly inhibited biofilm formation in 7/10 strains, while inhibition with 125 µg/ml of 5OF and 5CF3 was moderate. Lower concentrations had almost no effect on biofilm production. Pyocyanin production was reduced to less than 40% of the control value in 6/9, and less than 50% of the control in 7/9 strains with 500 μg/ml of 5OF and 5CF3, respectively. At 250 µg/ml 5OF and 5CF3, most strains had pyocyanin production above 50% of the control value. Conclusion Synthetized propafenone derivatives, 5OF and 5CF3, inhibited biofilms and pyocyanin production of Pseudomonas aeruginosa clinical strains. Presented results suggest that propafenone derivatives are potential lead-compounds for synthesis of novel antipseudomonal drugs.

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“…This is reflected in current therapies and also in ‘advanced’ therapeutic options, e.g. therapeutic monoclonal antibodies, both of which essentially try to suppress the immune system of patients .…”

Section: Introductionmentioning

confidence: 99%

The potential role of retroviruses in autoimmunity

2015

Immunological Reviews

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In the last 20 years research in Immunology underwent fundamental changes. Most importantly, the identification of the key role of innate immune pattern recognition receptors (PRRs) that recognize evolutionarily conserved molecular patterns on infectious pathogens. This results in priming of innate immune cells, which in turn activate and direct the adaptive immune response. Progress in innate immune recognition instigated the current working hypothesis, that recognition of endogenous ligands by PRRs results in innate immune cell activation (autoinflammation) or activation of adaptive cells, with self-reactive antigen receptors (autoimmunity). In particular, nucleic acid-sensing innate immune receptors seem to be prime candidates for a mechanistic understanding of autoreactive activation of the immune system. However, it remains uncertain what the actual source of nucleic acid ligands is and what other signals are needed to drive activation of autoreactive innate immune cells and break self-tolerance of the adaptive immune system. Here, I will review our present understanding about whether the infection with exogenous retroviruses or the reactivation of endogenous retroviruses might play an etiological role in certain autoimmune conditions of humans and murine experimental models.

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Optimizing pharmacotherapy of systemic lupus erythematosus: the pharmacist role

Abstract: The diagnosis of SLE is based on criteria set by the American College of Rheumatology. Management is individualized and depends on presenting symptoms and reducing the likelihood of permanent damage to organs and tissues.

Cited by 7 publications

References 58 publications

Vitamin D3 Alters the Expression of Toll‐like Receptors in Peripheral Blood Mononuclear Cells of Patients With Systemic Lupus Erythematosus

Vitamin D3 Alters the Expression of Toll‐like Receptors in Peripheral Blood Mononuclear Cells of Patients With Systemic Lupus Erythematosus

Inhibitory effect of propafenone derivatives on pseudomonas aeruginosa biofilm and pyocyanin production

The potential role of retroviruses in autoimmunity

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