Optimizing pear micropropagation and rooting with light emitting diodes and trans-cinnamic acid

Lotfi, Mariem; Mars, Messaoud; Werbrouck, Stefaan

doi:10.1007/s10725-019-00498-y

Cited by 27 publications

(35 citation statements)

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“…Mineral and hormone composition of a plant micropropagation medium are essential factors in explant growth [ 17 , 18 ]. Plant species differ in nutrient and hormone requirements, leading to development of numerous media formulations.…”

Section: Introductionmentioning

confidence: 99%

“…Choosing a medium based on prior usage may not give the same result response in other plant species [ 21 , 22 ]. Improper mineral or hormone concentrations can cause growth inhibition or various physiological disorders including leaf chlorosis, shoot tip necrosis, hyperhydricity, and leaf spots [ 10 , 17 , 18 ]. Understanding the effects of minerals, hormones, and their interaction with other media ingredients and cultured tissues, is essential for successful in vitro plant propagation [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

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High throughput mathematical modeling and multi-objective evolutionary algorithms for plant tissue culture media formulation: Case study of pear rootstocks

et al. 2020

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Simplified prediction of the interactions of plant tissue culture media components is of critical importance to efficient development and optimization of new media. We applied two algorithms, gene expression programming (GEP) and M5’ model tree, to predict the effects of media components on in vitro proliferation rate (PR), shoot length (SL), shoot tip necrosis (STN), vitrification (Vitri) and quality index (QI) in pear rootstocks (Pyrodwarf and OHF 69). In order to optimize the selected prediction models, as well as achieving a precise multi-optimization method, multi-objective evolutionary optimization algorithms using genetic algorithm (GA) and particle swarm optimization (PSO) techniques were compared to the mono-objective GA optimization technique. A Gamma test (GT) was used to find the most important determinant input for optimizing each output factor. GEP had a higher prediction accuracy than M5’ model tree. GT results showed that BA (Γ = 4.0178), Mesos (Γ = 0.5482), Mesos (Γ = 184.0100), Micros (Γ = 136.6100) and Mesos (Γ = 1.1146), for PR, SL, STN, Vitri and QI respectively, were the most important factors in culturing OHF 69, while for Pyrodwarf culture, BA (Γ = 10.2920), Micros (Γ = 0.7874), NH4NO3 (Γ = 166.410), KNO3 (Γ = 168.4400), and Mesos (Γ = 1.4860) were the most important influences on PR, SL, STN, Vitri and QI respectively. The PSO optimized GEP models produced the best outputs for both rootstocks.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

High throughput mathematical modeling and multi-objective evolutionary algorithms for plant tissue culture media formulation: Case study of pear rootstocks

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“…In recent work, Lotfi et al (2019) demonstrated that trans -cinnamic acid, a phenylpropenoic compound produced by the microbial deamination of phenylalanine ( Hazir et al, 2017 ), efficiently induces AR formation in shoots of pear cv Arbi. It is noteworthy that P. agglomerans C1 has genes encoding enzymes (e.g., a CAT-catalase, ec 1.11.1.6) that are involved in the biosynthesis of cinnamic-related compounds from intermediates of the tryptophan pathway, and we have evidence that strain C1 produces cinnamates as well as peptides and cyclopeptides that can crosstalk with auxin ( Luziatelli et al, 2020b ).…”

Section: Discussionmentioning

confidence: 99%

Metabolites Secreted by a Plant-Growth-Promoting Pantoea agglomerans Strain Improved Rooting of Pyrus communis L. cv Dar Gazi Cuttings

et al. 2020

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Strains belonging to Pantoea agglomerans species are known for their ability to produce metabolites that can act in synergy with auxins to induce the adventitious root (AR) formation. The latter is critically important in the agamic propagation of several woody species, including pear ( Pyrus communis L.), playing a considerable role in the commercial nursery farms including those using micropropagation techniques. When grown on a medium amended with tryptophan, the plant-growth-promoting (PGP) strain P. agglomerans C1 produces a cocktail of auxin and auxin-like molecules that can be utilized as biostimulants to improve the rooting of vegetable ( Solanum lycopersicum L.) and woody crop species ( Prunus rootstock GF/677 and hazelnut). In this study, we evaluated the morphological and molecular responses induced by strain C1 exometabolites in microcuttings of P. communis L. cv Dar Gazi and the potential benefits arising from their application. Results showed that exometabolites by P. agglomerans C1 induced a direct and earlier emergence of roots from stem tissues and determined modifications of root morphological parameters and root architecture compared to plants treated with the synthetic hormone indole-3-butyric acid (IBA). Transcription analysis revealed differences in the temporal expression pattern of ARF17 gene when IBA and C1 exometabolites were used alone, while together they also determined changes in the expression pattern of other key auxin-regulated plant genes. These results suggest that the phenotypic and molecular changes triggered by P. agglomerans C1 are dependent on different stimulatory and inhibitory effects that auxin-like molecules and other metabolites secreted by this strain have on the gene regulatory network of the plant. This evidence supports the hypothesis that the strategies used to harness the metabolic potential of PGP bacteria are key factors in obtaining novel biostimulants for sustainable agriculture. Our results demonstrate that metabolites secreted by strain C1 can be successfully used to increase the efficiency of micropropagation of pear through tissue culture techniques.

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“…Sobrevivência ( Research, Society and Development, v. 10, n. 2, e48510212790, 2021 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v10i2.12790 6 na ausência de 2ip no meio de cultivo (Tabela 2). O inverso ocorreu no experimento realizado por Lotfi et al (2019), onde utilizaram a citocinina meta-topolina (m TR) e verificaram uma menor área foliar sob luz fluorescente branca. Isto pode provar que há diferenças entre espécies, ou até mesmo o tipo de citocinina utilizada pode ter sido o principal agente que causou as diferenças entre os experimentos.…”

Section: Tratamentosunclassified

“…Como alternativa às fontes de luz utilizadas tradicionalmente, foram introduzidos na propagação de plantas os diodos emissores de luz (LEDs), mais especificamente no cultivo de plantas micropropagadas, uma vez que, diferentes estudos mostram plantas in vitro mais vigorosas cultivadas sob condições de iluminação por LED (Moon et al 2006;Lin et al 2011;Hung et al 2015;Batista et al 2016;Ferreira et al 2017;Batista et al, 2018). Estas vêm despertando o interesse de pesquisadores, que realizaram estudos sobre a aplicabilidade de diodos emissores de luz de amplo espectro (LEDs) para a micropropagação de espécies de plantas ornamentais (Miler et al, 2019), pereira (Lotfi et al, 2019) e bananeira (Musa spp.) (Bhaya & Al-Razzaqsalim, 2019), os quais mostraram resultados promissores.…”

Section: Introductionunclassified

Qualidade de luz na multiplicação e enraizamento in vitro de mirtileiro ‘Woodard’

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O objetivo deste estudo foi avaliar o efeito da qualidade da luz e dos reguladores de crescimento 2-isopentenil adenina (2-ip) e ácido indol 3-butírico (AIB) na multiplicação in vitro e enraizamento do mirtilo ‘Woodard’. Os experimentos foram organizados em um delineamento inteiramente casualizado, com três níveis de qualidade de luz (vermelho, azul e branco) e dois níveis de regulador de crescimento vegetal (presença ou ausência de 100 mg L-1 2-ip ou 0,5 mg L-1 AIB, nos experimentos de multiplicação e enraizamento, respectivamente). Os parâmetros avaliados em ambos os experimentos foram taxa de sobrevivência (%), número de brotos por explante, comprimento dos brotos (cm); número de folhas por explante e área foliar (cm2). No experimento de multiplicação também foi quantificado o conteúdo de pigmentos fotossintéticos [clorofila (chl) a e chl b, chl a + b, e carotenóides] e no ensaio de enraizamento o número e comprimento da raiz. Os resultados mostram que a presença de 2-ip em associação com a luz branca resulta em brotações mais longas, bem como em maior número de brotos e folhas. A maior área foliar foi observada com luz branca. O número e o comprimento da raiz foram maiores na presença de AIB no meio. A associação de luz fluorescente branca e a presença de 2-isopentenil adenina (2-ip) ou ácido indol 3-butírico (AIB) no meio fornece os melhores resultados para multiplicação in vitro e enraizamento, respectivamente, de 'Woodard' mirtilo.

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Optimizing pear micropropagation and rooting with light emitting diodes and trans-cinnamic acid

Cited by 27 publications

References 36 publications

High throughput mathematical modeling and multi-objective evolutionary algorithms for plant tissue culture media formulation: Case study of pear rootstocks

High throughput mathematical modeling and multi-objective evolutionary algorithms for plant tissue culture media formulation: Case study of pear rootstocks

Metabolites Secreted by a Plant-Growth-Promoting Pantoea agglomerans Strain Improved Rooting of Pyrus communis L. cv Dar Gazi Cuttings

Qualidade de luz na multiplicação e enraizamento in vitro de mirtileiro ‘Woodard’

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