Optimizing parameters on alignment of PCL/PGA nanofibrous scaffold: An artificial neural networks approach

Paskiabi, Farnoush Asghari; Mirzaei, Esmaeil; Amani, Amir; Shokrgozar, Mohammad Ali; Saber, Reza; Faridi‐Majidi, Reza

doi:10.1016/j.ijbiomac.2014.10.040

Cited by 26 publications

(14 citation statements)

References 36 publications

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“…Scaffolds based on PGA/PCL mixtures have recently merited attention from different points of views as for example: the evaluation of physical and mechanical properties of electrospun fibers, preparation of scaffolds with aligned fibers for neural applications and in vitro degradation studies …”

Section: Introductionmentioning

confidence: 99%

Scaffolds with Tunable Properties Constituted by Electrospun Nanofibers of Polyglycolide and Poly(ε‐caprolactone)

Keridou

Franco

Turón

et al. 2018

Macro Materials & Eng

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Electrospun scaffolds constituted by different mixtures of two biodegradable polyesters are prepared. Specifically, materials with well differentiated properties can be derived from the blending of hydrophilic polyglycolide (PGA) and hydrophobic poly(ε‐caprolactone) (PCL), which are also two of the most applied polymers for biomedical uses. Electrospinning conditions are selected in order to get homogeneous and continuous fibers with diameters in the nano/micrometric range. These conditions are also applied to load the different scaffolds with curcumin (CUR) and polyhexamethylene biguanide (PHMB) as hydrophobic and hydrophilic bactericide compounds, respectively. Physicochemical characterization of both unloaded and loaded scaffolds is performed and involved Fourier transform infrared and 1H NMR spectroscopies, morphological observations by scanning electron microscopy, study of thermal properties through calorimetry and thermogravimetric analysis, and evaluation of surface characteristics through contact angle measurements. Release behavior of the loaded scaffolds is evaluated in two different media. Results point out a well differentiated behavior where the delivery of CUR and even PHMB are highly dependent on the PGA/PCL ratio, the capability of the medium to swell the polymer matrix, and the diffusion of the selected solvent into the electrospun fibers. All samples show a bactericide effect in both hydrophilic cell culture and hydrophobic agar media.

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Section: Introductionmentioning

confidence: 99%

Scaffolds with Tunable Properties Constituted by Electrospun Nanofibers of Polyglycolide and Poly(ε‐caprolactone)

Keridou

Franco

Turón

et al. 2018

Macro Materials & Eng

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“…In order to produce novel scaffolds, biodegradable polymers have been extensively investigated [10]. Among them, synthetic aliphatic polyesters stand out due to their controllable biodegradability (PLA [11,12], PGA [13,14], PLGA [15,16], and PCL [13,17]). However, these polyesters are usually rigid and highly crystalline, which may impair some in vivo interactions.…”

Section: Introductionmentioning

confidence: 99%

Electrospun ultrathin PBAT/nHAp fibers influenced the in vitro and in vivo osteogenesis and improved the mechanical properties of neoformed bone

Santana-Melo

Rodrigues

Silva

et al. 2017

Colloids and Surfaces B: Biointerfaces

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Combining polyester scaffolds with synthetic nanohydroxyapatite (nHAp), which is bioactive and osteoconductive, is a plausible strategy to improve bone regeneration. Here, we propose the combination of PBAT [poly(butylene-adipate-co-terephthalate)] and synthetic nHAp (at 3 and 5wt%). PBAT is a relatively a new polymer with low crystallinity and attractive biodegradability and mechanical properties for orthopedic applications, however, with a still underexplored potential for in vivo applications. Then, we performed a careful biological in vitro and in vivo set of experiments to evaluate the influence of PBAT containing two different nHAp loads. For in vitro assays, osteoblast-like MG63 cells were used and the bioactivity and gene expression related to osteogenesis were evaluated by qRT-PCR. For in vivo experiments, twenty-four male rats were used and a tibial defect model was applied to insert the scaffolds. Micro-computed tomography (Micro-CT) and histological analysis were used to assess e bone neoformation after 6 weeks of implantation. Three point flexural tests measured the mechanical properties of the neoformed bone. All scaffolds showed promising in vitro properties, since they were not cytotoxic against MG-63 cells and promoted high cell proliferation and formation of mineralized nodules. From a mechanistic point-of-view, nHAp loading increased hydrophilicity, which in turn allowed for a better adsorption of proteins and consequent changes in the phenotypic expression of osteoblasts. nHAp induced better cellular responses on/in the scaffolds, which was mainly attributed to its osteoconductive and osteoinductive properties. Micro-CT images showed that nHAp at 3% and 5wt% led to more effective bone formation, presenting the highest bone volume after 6 weeks of implantation. Considering the three point flexural tests, 5wt% of nHAp positively influenced the flexural mode of the neoformed bone, but the stiffiness was similar between the 3% and 5wt% groups. In summary, this investigation demonstrated great potential for the application of these novel scaffolds towards bone regeneration and, thus, should be further studied.

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“…The ES method has been successfully used to prepare a variety of organic and inorganic fibrous structure morphologies, including beaded, core–shell, hollow, and porous . The high surface‐to‐volume ratio and the anisotropy of electrospun fibers make them ideal for several applications, such as drug delivery, wound dressings, tissue engineering, and energy storage . However, controlling fiber uniformity for aligned structure deposition remains a challenge, especially for multiaxial deposition.…”

Section: Introductionmentioning

confidence: 99%

Impact of substrate geometry on electrospun fiber deposition and alignment

Wang

Zhou

Chang³

et al. 2017

J of Applied Polymer Sci

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Aligned, uniform fiber matrixes are highly desirable in numerous engineering and physical science applications. Here, modified electrospinning (ES) deposition substrates (paired and in parallel) are explored to achieve rapid preparation of multiple topographies. Three ES substrates with well-defined geometries (rectangular, concave, and E-shaped) were investigated (arranged in parallel) for their impact on fiber size, morphology, orientation, and cell behavior. The results indicate fiber alignment and orientation can be improved and modulated based on the substrate geometry. In addition, altering the interdistance space between various parallel substrates has a clear impact on fiber diameter size and alignment (random, aligned, and perpendicular orientation). Electric field simulations based on substrate geometries show greater probable regions of aligned electric field vectors and distribution, which indicates the most likely deposition attributes of electrospun PCL fibers. Fibrous PCL membranes were biocompatible, and cell growth and guidance were along the fiber path, with evidence of branching at intersecting fibers for multiaxial fibrous topographies. These findings show that the substrate geometry can be optimized to effectively assemble multiaxial layered and well-aligned fibers in a controlled fashion, which is ideal to support several application developments dependent on fiber topography, integrity, and morphology.

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Optimizing parameters on alignment of PCL/PGA nanofibrous scaffold: An artificial neural networks approach

Cited by 26 publications

References 36 publications

Scaffolds with Tunable Properties Constituted by Electrospun Nanofibers of Polyglycolide and Poly(ε‐caprolactone)

Scaffolds with Tunable Properties Constituted by Electrospun Nanofibers of Polyglycolide and Poly(ε‐caprolactone)

Electrospun ultrathin PBAT/nHAp fibers influenced the in vitro and in vivo osteogenesis and improved the mechanical properties of neoformed bone

Impact of substrate geometry on electrospun fiber deposition and alignment

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