BACKGROUND: Owing to the advantages of high-efficiency, environmental protection and safety, nanoemulsions have become a highly popular water-insoluble pesticide delivery system in recent years. RESULTS: In this study, abamectin-loaded nanoemulsion with remarkable physical stability and application performance was obtained by selecting the type and concentration of surfactant and the emulsification method. The optimal formula was 2% abamectin and 5% castor oil polyoxyethylene (EL-40) dissolved in 7.5% hydrocarbon solvent (S-200) made up to 100% with deionized water, which conformed to the quality indicators from the Food and Agriculture Organization (FAO) standards. Droplets on cabbage leaves showed a small dynamic contact angle, which ensured that the resulting nanoemulsion exhibited excellent wettability and diffusivity. Compared with emulsifiable concentrates (EC) and microemulsions (ME), the abamectin-loaded nanoemulsion had the lowest LC 50 (0.0686 mg L −1) to third-instar larva of Plutella xylostella, causing the larval body to blacken and shrivel, which improved insecticidal activity. In addition, the abamectin-loaded nanoemulsion had low cytotoxicity. The viability of dendritic cells with added nanoemulsion reached 100% after 2 h, whereas that of cells with EC and ME was lower. After 24 h, the cell viability of dendritic cells with added ME was 0. CONCLUSION: This research facilitated the design and fabrication of nanoemulsions for water-insoluble pesticide to enhance insecticidal activity, lower cytotoxicity and reduce environmental pollution.