Optimizing mass production of Trichoderma asperelloides by submerged liquid fermentation and its antagonism against Sclerotinia sclerotiorum

Rezende, Larissa Castro de; Costa, L. B.; Halfeld-Vieira, B. de A.; Silva, Lucas Guedes; Pinto, Z. V.; Morandi, M. A. B.; Medeiros, Flávio Henrique Vasconcelos de; Mascarin, Gabriel Moura; Bettiol, W.

doi:10.1007/s11274-020-02882-7

Cited by 18 publications

(15 citation statements)

References 30 publications

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“…Trichoderma harzianum LQC‐99 and Trichoderma asperelloides LQC‐96, which exhibit antagonism towards plant pathogens (Rezende et al) 20 were obtained from the Collection of Microorganisms of Agricultural and Environmental Importance (CMAA) at Embrapa Meio Ambiente (Jaguariúna, São Paulo, Brazil). Aspergillus niger C, which has a high capacity to solubilize phosphate, 13 was obtained from Embrapa Agro Indústria de Alimentos (Rio de Janeiro, Brazil).…”

Section: Methodsmentioning

confidence: 99%

Bio‐based composite granules with simultaneous biocontrol and phosphorus fertilization roles: Outcomes from a lab‐scale in vitro assessment

Favaro

Klaic

Bettiol

et al. 2022

Biotechnology Progress

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The use of phosphate rocks as low‐solubility phosphorus fertilizers has been promoted to reduce the environmental impacts of agriculture, but adequate nutrient uptake by plants depends on solubilization of the rock, driven by soil microorganisms. Here, investigation was made of the microbial solubilization of low‐solubility phosphate rocks, together with simultaneous bioprotective action involving the biocontrol of microorganisms. The aim was to enhance function and value by delivering two effects using a single bio‐based product, in accordance with the concept of a “bioreactor‐in‐a‐granule” system. A composite structure was developed, based on a starch matrix, comprising a combination of Trichoderma asperelloides, as a biocontrol agent, and Aspergillus niger, as an acidulant. A significant increase of up to 150% in P solubilization was achieved, indicating the positive effect of the microorganism‐composite interaction. In vitro assays showed that the ability of T. asperelloides to inhibit Fusarium oxysporum mycelial growth was maintained in the presence of A. niger. Moreover, the estimated cost of the composite granule (0.35 US$/kg of product on a dry basis) revealed competitive. The results indicated that the association of T. asperelloides and A. niger is an effective way to increase nutrient availability and to inhibit plant pathogens, opening up possibilities for the design of multifunctional bio‐based fertilizer composites.

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Section: Methodsmentioning

confidence: 99%

Bio‐based composite granules with simultaneous biocontrol and phosphorus fertilization roles: Outcomes from a lab‐scale in vitro assessment

Favaro

Klaic

Bettiol

et al. 2022

Biotechnology Progress

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“…As a specific volatile substance of Trichoderma, 6-PP has shown strong antifungal activity [32]. In addition, the fermentation extract and volatile gases of T. asperellum have shown biocontrol activities and growth-promoting capabilities [33,34]. The use of Trichoderma and other filamentous fungi as biological control agents is a promising and durable biocontrol strategy against plant-parasitic nematodes in agriculture [20].…”

Section: Introductionmentioning

confidence: 99%

The Endophytic Strain Trichoderma asperellum 6S-2: An Efficient Biocontrol Agent against Apple Replant Disease in China and a Potential Plant-Growth-Promoting Fungus

Wang

Zhang

Duan

et al. 2021

JoF

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A study was conducted for endophytic antagonistic fungi obtained from the roots of healthy apple trees growing in nine replanted orchards in Shandong Province, China. The fungi were assessed for their ability to inhibit Fusarium proliferatum f. sp. malus domestica MR5, a fungal strain associated with apple replant disease (ARD). An effective endophyte, designated as strain 6S-2, was isolated and identified as Trichoderma asperellum. Strain 6S-2 demonstrated protease, amylase, cellulase, and laccase activities, which are important for the parasitic and antagonistic functions of pathogenic fungi. The inhibition rate of 6S-2 against Fusarium proliferatum f. sp. malus domestica MR5 was 52.41%. Strain 6S-2 also secreted iron carriers, auxin, ammonia and was able to solubilize phosphorus. Its fermentation extract and volatile substances inhibited the growth of MR5, causing its hyphae to twist, shrink, swell, and rupture. The antifungal activity of the 6S-2 fermentation extract increased with increasing concentrations. It promoted the production and elongation of Arabidopsis thaliana lateral roots, and the strongest effects were seen at a concentration of 50 mg/mL. A GC-MS analysis of the 6S-2 fermentation extract and volatile substances showed that they comprised mainly alkanes, alcohols, and furanones, as well as the specific volatile substance 6-PP. The application of 6S-2 spore suspension to replanted apple orchard soils reduced plant oxidative damage and promoted plant growth in a pot experiment. Therefore, the endophytic strain T. asperellum 6S-2 has the potential to serve as an effective biocontrol fungus for the prevention of ARD in China, and appears to promote plant growth.

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“…Alternatively, in recent years, important progress has been made in biological technology to control Sclerotinia stem rot. For example, the following microorganisms have been reported to exert antagonistic effects on S. sclerotiorum: Coniothyrium minitans (Mcquilken et al, 2010), Trichoderma viride (Rezende et al, 2020), Trichoderma harzianum (Rezende et al, 2020), Bacillus subtilis (Zhang et al, 2006), Streptomyces avermitilis (Ge et al, 2017), Streptomyces hygroscopicus (Ge et al, 2017), and sshadv-1 virus (Qu et al, 2021) Many studies have suggested that bacteria could produce substances that inhibit growth of other microorganisms (Arrebola et al, 2010). For example, B. amyloliquefaciens PPCB004 was characterized for its antifungal activity against seven selected fungal postharvest pathogens of citrus, including Alternaria citri, Botryosphaeria sp., and Colletotrichum gloeosporioides.…”

Section: Discussionmentioning

confidence: 99%

“…Alternatively, in recent years, important progress has been made in biological technology to control Sclerotinia stem rot. For example, the following microorganisms have been reported to exert antagonistic effects on S. sclerotiorum : Coniothyrium minitans ( Mcquilken et al, 2010 ), Trichoderma viride ( Rezende et al, 2020 ), Trichoderma harzianum ( Rezende et al, 2020 ), Bacillus subtilis ( Zhang et al, 2006 ), Streptomyces avermitilis ( Ge et al, 2017 ), Streptomyces hygroscopicus ( Ge et al, 2017 ), and sshadv-1 virus ( Qu et al, 2021 ). However, the control effects of these microbes on Sclerotinia stem rot have not yet been verified in field experiments.…”

Section: Discussionmentioning

confidence: 99%

Dual RNA Sequencing Analysis of Bacillus amyloliquefaciens and Sclerotinia sclerotiorum During Infection of Soybean Seedlings by S. sclerotiorum Unveils Antagonistic Interactions

Cheng

Gao

et al. 2022

Front. Microbiol.

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Soybean Sclerotinia stem rot is caused by Sclerotinia sclerotiorum infection, which causes extensive and severe damage to soybean production. Here, we isolated and patented a Bacillus amyloliquefaciens strain, and used it to verify the antagonistic effect of B. amyloliquefaciens on S. sclerotiorum and to explore the possible underlying mechanism. First, we conducted a plate confrontation experiment using the two microbes. Then, inoculation of soybean (Glycine max) seedlings with S. sclerotiorum (Gm-Ss), B. amyloliquefaciens (Gm-Ba), and their combination (Gm-Ba-Ss) was performed, followed by dual RNA sequencing analysis. Plate confrontation and inoculation experiments showed that B. amyloliquefaciens significantly antagonized S. sclerotiorum growth. The average number of fragments per kilobase of transcript per million fragments mapped of S. sclerotiorum transcripts in Gm-Ss and Gm-Ba-Ss inoculation treatments were 117.82 and 50.79, respectively, indicating that B. amyloliquefaciens strongly inhibited gene expression of S. sclerotiorum. In contrast, the average number of fragments per kilobase of transcript per million fragments mapped of B. amyloliquefaciens transcripts in Gm-Ba and Gm-Ba-Ss inoculation treatments were 479.56 and 579.66, respectively, indicating that S. sclerotiorum promoted overall gene expression in B. amyloliquefaciens. For S. sclerotiorum, 507 upregulated and 4,950 downregulated genes were identified among 8,975 genes in the paired comparison Gm-Ba-Ss vs. Gm-Ss. These differentially expressed genes (DEGs) were significantly enriched in the ribosome (ko03010) KEGG pathway. Additionally, for B. amyloliquefaciens, 294 upregulated and 178 downregulated genes were identified among all 3,154 genes in the paired comparison Gm-Ba-Ss vs. Gm-Ba, and these DEGs were mainly and significantly enriched in metabolism-related KEGG pathways, including the citrate cycle (ko00020) and carbon metabolism (ko01200). We concluded that B. amyloliquefaciens inhibits the expression of genes encoding the ribosomal subunit of S. sclerotiorum, resulting in protein synthesis inhibition in S. sclerotiorum, and thus had a strong antagonistic effect on the fungus. This study provides a scientific basis for the biological control of S. sclerotiorum by B. amyloliquefaciens.

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Optimizing mass production of Trichoderma asperelloides by submerged liquid fermentation and its antagonism against Sclerotinia sclerotiorum

Cited by 18 publications

References 30 publications

Bio‐based composite granules with simultaneous biocontrol and phosphorus fertilization roles: Outcomes from a lab‐scale in vitro assessment

Bio‐based composite granules with simultaneous biocontrol and phosphorus fertilization roles: Outcomes from a lab‐scale in vitro assessment

The Endophytic Strain Trichoderma asperellum 6S-2: An Efficient Biocontrol Agent against Apple Replant Disease in China and a Potential Plant-Growth-Promoting Fungus

Dual RNA Sequencing Analysis of Bacillus amyloliquefaciens and Sclerotinia sclerotiorum During Infection of Soybean Seedlings by S. sclerotiorum Unveils Antagonistic Interactions

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