Optimizing light dosimetry in photodynamic therapy of early stage carcinomas of the esophagus using fluorescence spectroscopy

Braichotte, D.; Savary, Jean-François; Monnier, Philippe; Bergh, Hubert E. van den

doi:10.1002/(sici)1096-9101(1996)19:3<340::aid-lsm10>3.0.co;2-8

Cited by 84 publications

(41 citation statements)

References 11 publications

(4 reference statements)

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“…5 Several groups have investigated tissue fluorescence as a surrogate for actual tissue drug concentration, as a predictor of PDT tissue response. [6][7][8][9] In the present study we used before-PDT fluorescence as a means to characterize tis-*Current affiliation: U.T. MD Anderson Cancer Center, 1515 Holcombe Boulesue drug concentration.…”

Section: Introductionsupporting

confidence: 73%

“…2,18 For example, in PDT of early stage carcinomas of the esophagus with tetra͑meta-hydroxyphynyl͒chlorin ͑mTHPC͒, investigators have found large variations of mTHPC uptake among patients. 8 Fluctuations in the degree of tumor destruction between patients were found to be related to mucosal drug uptake as measured by LIF, 8 suggesting that LIF may be used as a guide for PDT. By using LIF as a measure of tissue photosensitizer concentration, these investigators improved the predictability of PDTinduced tumor destruction.…”

Section: Fluorescence/absorption Spectroscopymentioning

confidence: 99%

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Correlation of in vivo photosensitizer fluorescence and photodynamic-therapy-induced depth of necrosis in a murine tumor model

et al. 2003

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We compared light-induced fluorescence (LIF) to nominal injected drug dose for predicting the depth of necrosis response to photodynamic therapy (PDT) in a murine tumor model. Mice were implanted with radiation-induced fibrosarcoma (RIF) and were injected with 0, 5, or 10 mg/kg Photofrin. 630-nm light (30 J/cm(2), 75 mW/cm(2)) was delivered to the tumor after 24 hours. Fluorescence emission (lambda(excitation)=545 nm, lambda( emission)=628 nm) from the tumor was measured. The LIF data had less scatter than injected drug dose, and was found to be at least as good as an injected drug dose for predicting the depth of necrosis after PDT. Our observations provide further evidence that fluorescence spectroscopy can be used to quantify tissue photosensitizer uptake and to predict PDT tissue damage.

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Section: Introductionsupporting

confidence: 73%

Section: Fluorescence/absorption Spectroscopymentioning

confidence: 99%

Correlation of in vivo photosensitizer fluorescence and photodynamic-therapy-induced depth of necrosis in a murine tumor model

et al. 2003

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“…Several groups have used fluorescence methods to quantify photosensitizers in vivo in various tissues. Braichotte et al (18) quantified esophageal tetra(meta-hydroxyphenyl)chlorin with fluorescence and showed that the tissue level was related to treatment response. Glanzmann et al (19) also correlated mucosal content of tetra(meta-hydroxyphenyl)chlorin with PDT response.…”

Section: Discussionmentioning

confidence: 99%

Pilot Study on Light Dosimetry Variables for Photodynamic Therapy of Barrett's Esophagus with High-Grade Dysplasia

Gill

Wolfsen

Preyer

et al. 2009

Clinical Cancer Research

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Purpose: Photodynamic therapy (PDT) is used to treat Barrett's esophagus with high-grade dysplasia and mucosal carcinoma. Outcomes are variable with some patients having persistent disease, whereas others develop strictures. The aims of this study were (a) to compare porfimer sodium tissue uptake, light dose, and esophageal thickness with clinical outcomes and (b) to determine the selectivity of porfimer sodium uptake in diseased and normal epithelium. Experimental Design: Forty-eight hours after porfimer sodium infusion, patients underwent mucosal biopsy for quantification of the porfimer sodium. Laser light was delivered at 48 hours and again 24 or 48 hours later. Porfimer sodium was extracted from the biopsy samples and quantified using fluorescence spectroscopy. The enhanced photodynamic dose was determined as [porfimer sodium content * light dose/esophageal thickness]. PDT efficacy was determined 6 to 8 weeks later based on persistence or complete ablation of dysplasia or carcinoma. Results: Mean porfimer sodium content of 6.2 mg/kg (range, 2.6-11.2 mg/kg) and mean total light dose of 278 J/cm (range, 225-360 J/cm) resulted in a complete treatment. Mean porfimer sodium tissue content of 3.9 mg/kg (range, 2.1-8.1mg/kg) and mean total light dose of 268 J/cm (range, 250-350 J/cm) resulted in an incomplete treatment. The total esophageal thickness (range, 1.7-6.0 mm) and enhanced photodynamic dose were correlated with treatment outcome. Conclusions: Esophageal thickness is the strongest predictor of treatment outcome. The porfimer sodium content of Barrett's and normal tissue is not significantly different. ''Photodynamic dose'' for esophageal PDTshould incorporate the esophageal thickness.

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“…L is assumed to be the same before and after adding the absorber. 1s' (u2s' PCt'(O)) = I5(u' ,d,P(9))e (1) Assume that the molecules responsible for absorbance Pa also fluoresce. The intensity of the fluorescent light, If, is proportional to the amount of light that was absorbed by the fluorescent molecules and is given by Eq.…”

Section: Modelmentioning

confidence: 99%

<title>Optical measurement of photosensitizer concentration using a probe with a small source-detector fiber separation</title>

Canpolat

Mourant

2000

SPIE Proceedings

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We present a novel minimally invasive method to measure the concentration of photodynamic thearapy drugs in real time. The method is based on measurements of backscattered and fluorescence light using a steady state fluorescence spectrometer. The ratio of the fluorescence to scattered light is found to be linearly proportional to the absorption coefficient of the photosensitizer. The fiber-optic probe used for the measurements has a small source-detector separation, therefore the measurements could be performed through the working channel of an endoscope.

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Optimizing light dosimetry in photodynamic therapy of early stage carcinomas of the esophagus using fluorescence spectroscopy

Cited by 84 publications

References 11 publications

Correlation of in vivo photosensitizer fluorescence and photodynamic-therapy-induced depth of necrosis in a murine tumor model

Correlation of in vivo photosensitizer fluorescence and photodynamic-therapy-induced depth of necrosis in a murine tumor model

Pilot Study on Light Dosimetry Variables for Photodynamic Therapy of Barrett's Esophagus with High-Grade Dysplasia

<title>Optical measurement of photosensitizer concentration using a probe with a small source-detector fiber separation</title>

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