Optimizing Green Synthesis of Hydrotalcite – Silver Nanoparticles using Syzygium Nervosum based Reducing Agent

Abstract

This work aimed to assess the effect of Syzygium nervosum leaf extract as a reducing agent for green synthesis of AgNPs on HT through an optimizing process using response surface methodology (RSM) and the Box‐Benken model. The optimal conditions for synthesis of AgNPs on HT include reaction time of 6.15 hours, reaction temperature of 50 oC and the ratio of diluted Syzygium nervosum leaf extract to reduce AgNO3 of 50.37 mL/mg. Under the optimal conditions, the yield of reduction reaction reached 77.54 %, close to the theoretical value of 76.97 %. Besides, the morphology, density, and characteristics of AgNPs on the surface of HT layers have been determined by using Ultraviolet‐visible spectroscopy, Field emission scanning electron microscopy, High resolution transmission electron microscopy, selected area diffraction, X‐ray diffraction, Dynamic light scattering, Infrared spectroscopy, Fluorescence emission spectroscopy, Brunauer‐Emmett‐Teller methods. The spherical AgNPs were synthesized successfully on the surface of HT with the average particle size of 13.0 ± 1.1 nm. Interestingly, HT‐Ag hybrid materials can inhibit strongly the growth of E. coli, S. aureus as well as two antibiotic resistance bacterial strains, P. stutzeri B27 and antibiotic resistance E. coli. Overall, the HT‐Ag hybrid materials are very promising for application in practice.