Optimizing expression of transgenes with an emphasis on post-transcriptional events

Koziel, Michael G.; Carozzi, Nadine B.; Desai, Nalini

doi:10.1007/978-94-009-0353-1_18

Cited by 33 publications

(45 citation statements)

References 78 publications

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“…In vertebrates, initiation at non-AUG sites of some mRNAs can also be stimulated in the presence of specific nucleotides located downstream of the initiation codon, preferentially G at þ4, A or C at þ5 and U at þ6 (Grü nert and Jackson, 1994). Interestingly, this experimentally derived optimal downstream sequence is similar although not identical to the statistically derived preferential use of downstream nucleotides between positions þ4 and þ6 (GCU) of yeast and plant mRNAs (Galibert et al, 1996;Koziel et al, 1996).…”

Section: 'Consensus Sequences' That Influence the Selection Of Initiamentioning

confidence: 82%

“…GCU) found in yeast and plant mRNAs ( Fig. 2B; Galibert et al, 1996;Koziel et al, 1996) may help to correctly position a potential initiation codon in the decoding centre. Interestingly, the core region of the 18S rRNA decoding centre is nearly identical to the sequences in and around the anticodon loop of initiator tRNA (Fig.…”

Section: Internal Ribosome Entry: An Alternative Mechanism For Selectmentioning

confidence: 99%

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Functional importance of RNA interactions in selection of translation initiation codons

Sprengart

Porter

1997

Molecular Microbiology

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SummaryRNA base pairing between the initiation codon and anticodon loop of initiator tRNA is essential but not sufficient for the selection of the 'correct' mRNA translational start site by ribosomes. In prokaryotes, additional RNA interactions between small ribosomal subunit RNA and mRNA sequences just upstream of the start codon can efficiently direct the ribosome to the initiation site. Although there is presently no proof for a similar important ribosomal RNA interaction in eukaryotes, the 5Ј non-coding regions of their mRNAs and 'consensus sequences' surrounding initiation codons have been shown to be strong determinants for initiation-site selection, but the exact mechanisms are not yet understood. Intramolecular base pairing in mRNA and participation of translation initiation factors can strongly influence the formation of mRNA-small ribosomal subunit-initiator tRNA complexes and modulate translational activities in both prokaryotes and eukaryotes. Only recently has it been appreciated that alternative mechanisms may also contribute to the selection of initiation codons in all organisms. Although direct proof is currently lacking, there is accumulating evidence that additional cis-acting mRNA elements and trans-acting proteins may form specific 'bridging' interactions with ribosomes during translation initiation.

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Section: 'Consensus Sequences' That Influence the Selection Of Initiamentioning

confidence: 82%

Section: Internal Ribosome Entry: An Alternative Mechanism For Selectmentioning

confidence: 99%

Functional importance of RNA interactions in selection of translation initiation codons

Sprengart

Porter

1997

Molecular Microbiology

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“…Limitations such as codon usage also need to be considered. Modifying codon usage in some genes may increase the protein synthesis as well as eliminating the instability of sequences [38]. A possible strategy is to introduce inducible and tissue specific promoters using the chloroplast expression system.…”

Section: Discussionmentioning

confidence: 99%

Comparison of expression systems for the production of human interferon-α2b

Memari¹,

Ramanan

Ariff³

2010

Open Life Sciences

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Abstract:The production of human interferon alpha2b (IFN-α2b) in two expression systems, tobacco (Nicotiana tabaccum) and Escherichia coli, was compared in various aspects such as safety, yield, quality of product and productivity. In the E. coli system, IFN-α2b was expressed under a pelB signal sequence and a T7lac promoter in a pET 26b(+) vector. The same gene was also cloned in expression plant vector (pCAMBIA1304) between cauliflower mosaic virus promoter (CaMV35S) and poly A termination region (Nos) and expressed in transgenic tobacco plants. The expression of protein in both systems was confirmed by western immunoblotting and the quantity of the protein was determined by immunoassay. The amount of periplasmic expression in E. coli was 60 µg/L of culture, while the amount of nuclear expression in the plant was 4.46 µg/kg of fresh leaves. The result of this study demonstrated that IFN-α2b was successfully expressed in periplasm of bacterial and plant systems. The limitations on the production of IFN-α2b by both systems are addressed and discussed to form the basis for the selection of the appropriate expression platform.© Versita Sp. z o.o.

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“…Arber's view may be challenged, as gene exchange between species that are sexually not compatible (horizontal gene transfer) is a rare event in plants [18]. Furthermore, the integration of a bacterial gene such as the gene encoding the Bt-toxin to confer pest resistance is always done by embedding the bacterial gene into regulatory elements of eukaryotes to get expression of the transgene [19]. Such expression-targeted gene integration is most unlikely to occur by horizontal gene transfer.…”

Section: The Challenge By Gm Potatoesmentioning

confidence: 99%

I Have a Dream: Organic Movements Include Gene Manipulation to Improve Sustainable Farming

Ryffel

2017

Sustainability

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Several papers in a Special Issue of Sustainability have recently discussed various aspects to evaluate whether organic farming and gene manipulation are compatible. A special emphasis was given to new plant breeding techniques (NPBTs). These new approaches allow the most predictable genetic alterations of crop plants in ways that the genetically modified plant is identical to a plant generated by conventional breeding. The articles of the Special Issue present the arguments pro and contra the inclusion of the plants generated by NPBTs in organic farming. Organic movements have not yet made a final decision whether some of these techniques should be accepted or banned. In my view these novel genetically manipulated (GM) crops could be used in such a way as to respect the requirements for genetically manipulated organisms (GMOs) formulated by the International Federation of Organic Movements (IFOAM). Reviewing the potential benefits of disease-resistant potatoes and bananas, it seems possible that these crops support organic farming. To this end, I propose specific requirements that the organic movements should proactively formulate as their standards to accept specific GM crops.

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Optimizing expression of transgenes with an emphasis on post-transcriptional events

Cited by 33 publications

References 78 publications

Functional importance of RNA interactions in selection of translation initiation codons

Functional importance of RNA interactions in selection of translation initiation codons

Comparison of expression systems for the production of human interferon-α2b

I Have a Dream: Organic Movements Include Gene Manipulation to Improve Sustainable Farming

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