Optimizing culture conditions

Miller, Kathleen A.

doi:10.1017/cbo9781139059053.019

Cited by 2 publications

(1 citation statement)

References 37 publications

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“…Conversely, when aiming to deliberately change the medium composition and culture environment at the cleavage and blastocyst stage, respectively, sequential media offer benefits over a single medium. For example, the optimum pH of a medium may differ in the cleavage and blastocyst stages [107]. With a single medium, transfer of the medium to a separate incubator having a different CO 2 concentration, or change of incubator CO 2 concentrations, is needed for pH adjustment.…”

Section: Comparison Of Sequential Media With Single Mediummentioning

confidence: 99%

Human Preimplantation Embryo Culture Media: Past, Present, and Future

Yao

Asayama

2016

Journal of Mammalian Ova Research

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Systematic studies of mouse embryo culture beginning in 1949 led to an understanding of essential medium components for early mammalian embryos, and embryo culture from the zygote to the blastocyst stage was achieved in 1968. Since then, medium components that are either beneficial or detrimental for embryo culture have been identified. A variety of culture media that mimic the female reproductive tract, such as human tubal fluid medium and sequential media, were developed from the 1970s to the 1990s, and a single medium in which the concentrations of components were determined by a simplex optimization method was introduced for clinical use in 2002. While either sequential media or a single medium is currently used in most cases, no conclusion has yet been reached as to which of the two approaches is the best. That we are now easily able to culture embryos is the result of the work of pioneers. This review presents a chronological overview of media development from initial attempts at mouse embryo culture using synthetic media to the human embryo culture media used today. It also presents the characteristics of sequential media and a single medium. Finally, problems observed with current embryo culture media are discussed, along with future development in this area.

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Section: Comparison Of Sequential Media With Single Mediummentioning

confidence: 99%

Human Preimplantation Embryo Culture Media: Past, Present, and Future

Yao

Asayama

2016

Journal of Mammalian Ova Research

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Development of in vitro screening system for food habit related risk analysis

Bruch

Möhr²

2015

JEBAS

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In order to assess the health risk that associated with the consumption of unknown feed or food ingredients, there is a strong need of developing an in vitro screening system. The test system should be fast, reliable, inexpensive and without the necessity of performing animal tests. Furthermore, it should also provide important clues to the potential danger of unknown substances. The present study examines the extent to which cell and tissue cultures can be used for such studies. It should be ascertained whether the cell cultures can replace the native intestinal epithelium in terms of their sensitivity and provide accurate results as a quick "screening system". As a model for intestinal operations ex vivo tissue cultures from the native intestinal epithelium of the pig and the permanent cell line IPEC-J2 were used. The cell culture was characterized in terms of their morphological and functional properties (TEER, tight-junction proteins).Various studies (short-circuit measurements, translocation of [ 3 H]-mannitol) were performed to IPEC-J2 cells and the native intestinal epithelium in order to compare the functional properties of both systems. Finally, the response of the addition of "unknown" test substances (papain and wheat extract) were investigated to determine whether the functional parameters of both systems are affected by these test substances or not. The IPEC-J2 cells show a more significant influence in their functionality by "unknown" substances than the control variant. Results of study revealed that the in vitro system reacts rapidly in response of unknown test substances and it is more sensitive. Therefore, it is possible to operate a "risk assessment" for "unknown" substances with the help of this developed screening system.

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Optimizing culture conditions

Cited by 2 publications

References 37 publications

Human Preimplantation Embryo Culture Media: Past, Present, and Future

Human Preimplantation Embryo Culture Media: Past, Present, and Future

Development of in vitro screening system for food habit related risk analysis

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