Optimizing conditions for the continuous culture of Isochrysis affinis galbana relevant to commercial hatcheries

Abstract

Optimal culture conditions were investigated for the continuous culture of Isochrysis affinis galbana (T-Iso) by assessing the effects of irradiance, temperature, pH and nitrogen and phosphorus requirements. Highlights ► We investigated optimal conditions for Isochrysis affinis galbana (T-Iso). ► Irradiance efficiency is optimal for a given set of irradiance and dilution rate. ► pH-temperature and pH-irradiance interactions occur on growth. ► We propose an integrative optimization procedure for T-Iso continuous cultures.

Effect of N:P ratio on growth and chemical composition of Nannochloropsis oculata and Tisochrysis lutea

“…Cellular C contents of algae at the N:P ratios of 5:1 and 120:1 were high in T. lutea and N. oculata in the present study, in line with a previous result on C. leptoporus where lower N:P ratios of 0.17:1 and higher N:P ratios of 3000:1 increased C content compared with the N:P ratio of 22:1 (Langer et al 2012). Similarly, N:P ratios of 0.57:1 and 115:1 also increased the carbon content in T. lutea (Marchetti et al 2012). Under the optimum range of N:P ratios (20:1-30:1), carbon contents were lower than the high (120:1) and low N:P ratios (5:1) in T. lutea and N. oculata.…”

“…Microalgae are photosynthetic organisms that constitute the first primary link of an aquatic food chain (Marchetti et al 2012). The products of microalgae have various applications, particularly in pharmaceuticals, cosmetics, biofuel production, and aquaculture (Pulz and Gross 2004;Rosenberg et al 2008;Borowitzka 2013).…”

Dissolved azaspiracids are absorbed and metabolized by blue mussels (Mytilus edulis)

“…galbana (CCAP 927/14, T-Iso), is a non-toxic microalga used in aquaculture to feed farmed bivalve molluscs (Marchetti et al, 2012).…”

A simple spectrophotometric method for biomass measurement of important microalgae species in aquaculture

“…The maximum absorbance was achieved at the end of the stationary phase. Previous works reported values of μ: 0.225-0.665 d − 1 and dt: 3.080-1.042 d for Chaetoceros calcitrans (Phatarpekar et al, 2000), and values of μ: 0.33-1.97 d − 1 and dt: 2.100-0.352 d for I. affinis galbana (T-Iso), obtained by optimizing growth conditions (Marchetti et al, 2012). Regarding N. gaditana and P. tricornutum values of μ: 0.2-1.44 d − 1 , dt: 3.46-0.48 d, and of μ: 0.2-1.44 d −1 , dt: 3.46-0.48 d, respectively, have been also reported .…”