Optimizing compressive strength of sand treated with MICP using response surface methodology

Erdmann, Niklas; Payrebrune, Kristin M. de; Ulber, R.; Strieth, Dorina

doi:10.1007/s42452-022-05169-8

Cited by 8 publications

(6 citation statements)

References 46 publications

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“…Biocementation products such as bricks and columns are often used to measure strength and physical properties from the biocementation (Bachmeier et al, 2002;Zhao et al, 2014;Cardoso et al, 2020;Erdmann et al, 2022;Spencer et al, 2023). Compressive strength testing of cylindrical bricks made with S. pasteurii or E. coli INP-silicatein-α revealed encouraging results for the development of a silica-based biocementation pathway.…”

Section: Resultsmentioning

confidence: 99%

Surface-displayed silicatein-α enzyme in bioengineered E. coli enables biocementation and silica mineralization

Vigil,

Schwendeman,

Grogger

et al. 2024

Front. Syst. Biol.

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Biocementation is an exciting biomanufacturing alternative to common cement, which is a significant contributor of CO2 greenhouse gas production. In nature biocementation processes are usually modulated via ureolytic microbes, such as Sporosarcina pasteurii, precipitating calcium carbonate to cement particles together, but these ureolytic reactions also produce ammonium and carbonate byproducts, which may have detrimental effects on the environment. As an alternative approach, this work examines biosilicification via surface-displayed silicatein-α in bio-engineered E. coli as an in vivo biocementation strategy. The surface-display of silicatein-α with ice nucleation protein is a novel protein fusion combination that effectively enables biosilicification, which is the polymerization of silica species in solution, from the surface of E. coli bacterial cells. Biosilicification with silicatein-α produces biocementation products with comparable compressive strength as S. pasteurii. This biosilicification approach takes advantage of the high silica content found naturally in sand and does not produce the ammonium and carbonate byproducts of ureolytic bacteria, making this a more environmentally friendly biocementation strategy.

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Section: Resultsmentioning

confidence: 99%

Surface-displayed silicatein-α enzyme in bioengineered E. coli enables biocementation and silica mineralization

Vigil,

Schwendeman,

Grogger

et al. 2024

Front. Syst. Biol.

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“…In the literature [12,13,19,46,47], in most cases, sandy soils are used to conduct the MICP process, where the bacterial and cementation solution with the use of a peristaltic pump or other equipment, can easily flow through the soil particles. Mitchell & Santamarina [41] prepared a graph showing the bacteria size and types of soils, where the process of biomineralization may be a misguided soil improvement method.…”

Section: Sample Preparationmentioning

confidence: 99%

“…The method has been used in different ways in civil engineering [15], such as concrete crack repair [12,16], bioconcrete [11,17], sand consolidation [18,19], and soil improvement [20][21][22][23][24][25][26][27][28]. Referring to soil reinforcement, the following properties have been improved: shear strength [29,30], permeability [31,32], unconfined compressive strength [13,28,33,34], compressibility [35][36][37], liquefaction resistance [38,39].…”

Section: Introductionmentioning

confidence: 99%

Effect of Ureolytic Bacteria on Compressibility of the Soils with Variable Gradation

Wasil,

Wydro,

Wołejko

2023

Architecture, Civil Engineering, Environment

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The aim of this study is to present the effect of treatment with ureolytic bacteria (Sporosarcina pasteurii) on the compressibility parameters of mineral and anthropogenic soils. In the presence of the urease enzyme, secreted by a strain of Sporosarcina pasteurii bacteria, urea hydrolysis occurs, allowing CaCO3 to be precipitated. The literature suggests applying the Microbially Induced Calcite Precipitation (MICP) method to non-cohesive soils. In order to determine whether the biomineralization process occurs in other soil types, cohesive and anthropogenic soils were tested in the laboratory. Compressibility tests were carried out in the laboratory on MICP-treated and untreated soils as reference samples. The process of biocementation in the soil is made possible by the introduction of bacteria into the soil and subsequent activation by a cementation solution (consisting of urea and calcium ions Ca2+). This paper presents the methodology for introducing bacteria into the soil, as well as the effect of the biomineralization process on the deformation parameters of the tested materials.

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“…Microbiologically Induced Calcium Carbonate Precipitation (MICP) is a technology that got more and more attention in research during the last years [13]. Through different microbiological pathways calcium carbonate is precipitated.…”

Section: Introductionmentioning

confidence: 99%

“…Since mechanical parameters of biosandstone correlate with the calcium carbonate content in the sample [9,38,42], reproduceable results for MICP will therefore largely depend on achieving a homogenous calcium carbonate distribution. Research has described several biological, chemical and physical parameters that impact the nal results of MICP [2,13]. While a lot of these parameters like type and concentration of bacterial cells, temperature, composition of calcination solution, etc.…”

Section: Introductionmentioning

confidence: 99%

MICP treated sand: Insights into the impact of particle size on mechanical parameters and pore network after biocementation

Erdmann,

Schaefer,

Simon

et al. 2024

Preprint

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Microbiologically Induced Calcium Carbonate Precipitation (MICP) is a technology for improving soil characteristics, especially strength, that has been gaining increasing interest in literature during the last few years. Although a lot of influencing factors on the result of MICP are known, particle size and shape of the particles remain poorly understood. While destructive measuring of compressive strength or calcium carbonate content are important for the characterization of samples these methods give no insight into the internal structures and pore networks of the samples. X-ray microcomputed tomography (micro-CT) is a technique that is used to characterize the internals of rocks and to a certain degree MICP-treated soils. However, the impact of filtering and image processing of micro-CT Data depending on the type of MICP sample is poorly described in the literature. In this study, single fractions of local quarry were treated with MICP through the ureolytic microorganism Sporosarcina pasteurii to investigate the influence of particle size distribution on calcium carbonate content, unconfined compressive strength and the reduction of water permeability. Additionally, micro-CT was conducted to obtain insights into the resulting pore system. The impact of the Gauss filter und Non-local means filter on the resulting images and data on the pore network are discussed. The results show that particle size has a significant impact on the result of all tested parameters of biosandstone with lower particle size leading to higher strength and generally higher calcium carbonate content. Micro-CT data showed that the technology is feasible to gain valuable insights into the internal structures of biosandstone but the resolution and signal-to-noise ratio remain challenging, especially for samples with particle sizes smaller than 125 µm..

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Optimizing compressive strength of sand treated with MICP using response surface methodology

Cited by 8 publications

References 46 publications

Surface-displayed silicatein-α enzyme in bioengineered E. coli enables biocementation and silica mineralization

Surface-displayed silicatein-α enzyme in bioengineered E. coli enables biocementation and silica mineralization

Effect of Ureolytic Bacteria on Compressibility of the Soils with Variable Gradation

MICP treated sand: Insights into the impact of particle size on mechanical parameters and pore network after biocementation

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