Optimizing and Evaluating the Antihelminthic Activity of the Biocompatible Zinc Oxide Nanoparticles Against the Ascaridid Nematode, Parascaris equorum In Vitro

Morsy, Kareem; Fahmy, Sohair R.; Mohamed, Ayman Saber; Ali, Sara E.; El–Garhy, Manal; Shazly, Mohammed

doi:10.2478/s11686-019-00111-2

Cited by 16 publications

(14 citation statements)

References 58 publications

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“…Regarding the oxidative stress induction by CuO-NPs and ZnO-NPs in living organisms (Khan et al ., 2015; Esmaeilnejad et al ., 2018; Morsy et al ., 2019; Malekifard et al ., 2020), the current study aimed to assess oxidative/nitrosative stress biomarkers and DNA damage in adult M . marshalli after exposure to different concentrations of CuO-NPs and ZnO-NPs.…”

Section: Discussionmentioning

confidence: 99%

Toxicities of the copper and zinc oxide nanoparticles onMarshallagia marshalli(Nematoda: Trichostrongylidae): evidence on oxidative/nitrosative stress biomarkers, DNA damage and egg hatchability

et al. 2021

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This study investigated the in vitro anthelmintic activity of copper oxide (CuO) and zinc oxide (ZnO) nanoparticles (NPs) against Marshallagia marshalli. The in vitro study was based on an egg hatch assay, adult and larvae motility inhibition assays, DNA damage, intensity protein profile along with several oxidative/nitrosative stress biomarkers including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), protein carbonylation (PCO), malondialdehyde (MDA), total antioxidant status (TAS) and nitric oxide (NO) content. Different concentrations of CuO-NPs and ZnO-NPs (1, 4, 8, 12 and 16 ppm) were used to assess anthelmintic effects on three stages of M. marshalli life cycle – that is, eggs, larvae and adult parasites for 24 h. The results indicated that CuO-NPs and ZnO-NPs played a significant role as anthelminthics, and the effect was dependent on time and concentration. The concentrations of 12 and 16 ppm of CuO-NPs and 16 ppm of ZnO-NPs resulted in the induction of oxidative/nitrosative stress (decreased SOD, GSH-Px and CAT, and increased MDA, PCO and NO), increased DNA damage, inhibition of adult and larval motility, egg hatch and low intensity of protein bands following sodium dodecyl sulphate–polyacrylamide gel electrophoresis, compared to control. It was concluded that CuO-NPs and ZnO-NPs could be utilized as novel and potential agents for the control and treatment of M. marshalli infection, and they have the pharmacological potential to be studied in vivo for further utilization in treating parasitic infections.

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Section: Discussionmentioning

confidence: 99%

Toxicities of the copper and zinc oxide nanoparticles onMarshallagia marshalli(Nematoda: Trichostrongylidae): evidence on oxidative/nitrosative stress biomarkers, DNA damage and egg hatchability

et al. 2021

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“…Wild tarragon ( Artemisia dracunculus , Linnaeus, 1753), pennyrile ( Mentha pulegium , Linnaeus, 1753), Zataria multiflora (Boiss), cinnamon ( Cinnamomum zeylanicum , Blume), pomegranate flower ( Punica granatum , Linnaeus, 1753), and pepper ( Capsicum annuum , Linnaeus, 1753) extracts were all lethal to L2/L3 larval Parascaris in vitro (Rakhshandehroo et al 2016 , 2017 ). Zinc oxide nanoparticles showed in vitro anthelmintic efficacy against Parascaris spp., including changes to morphological appearance (Morsy et al 2019 ). The monoterpenic phenol isomer carvacrol, isolated from herbs, also showed in vitro anthelmintic activity against Parascaris spp.…”

Section: The Biology Of Resistancementioning

confidence: 99%

The equine ascarids: resuscitating historic model organisms for modern purposes

Cain

Nielsen

2022

Parasitol Res

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The equine ascarids, Parascaris spp., are important nematode parasites of juvenile horses and were historically model organisms in the field of cell biology, leading to many important discoveries, and are used for the study of chromatin diminution. In veterinary parasitology, Parascaris spp. are important not only because they can cause clinical disease in young horses but also because they are the only ascarid parasites to have developed widespread anthelmintic resistance. Despite this, much of the general biology and mechanisms of anthelmintic resistance are poorly understood. This review condenses known basic biological information and knowledge on the mechanisms of anthelmintic resistance in Parascaris spp., highlighting the importance of foundational research programs. Although two variants of this parasite were recognized based on the number of chromosomes in the 1870s and suggested to be two species in 1890, one of these, P. univalens , appears to have been largely forgotten in the veterinary scientific literature over the past 100 years. We describe how this omission has had a century-long effect on nomenclature and data analysis in the field, highlighting the importance of proper specimen identification in public repositories. A summary of important basic biology, including life cycle, in vitro maintenance, and immunology, is given, and areas of future research for the improvement of knowledge and development of new systems are given. Finally, the limited knowledge regarding anthelmintic resistance in Parascaris spp. is summarized, along with caution regarding assumptions that resistance mechanisms can be applied across clades.

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“…Improvement in biomedical nanotechnology leads to many exciting applications such as drug discovery, drug delivery, and gene/protein delivery (Nazir, et al, 2019). Nanoparticles (NPs) have different properties, depending on their size, shape, and morphology, allowing them to interact with microorganisms, plants, and animals (Morsy, et al, 2019). Moreover, nanoparticles are more highly absorbed into the respiratory, skin, and gastrointestinal systems than micron-sized particles (Balogun, et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Zinc (Zn) is a fundamental element for human and animal health but is toxic to microorganisms. ZnObased nanomaterials have developed enormous importance in nanomedicine and have practical biomedical applications due to their low cost, high stability, wide bandgap, small size, and physicochemical properties (Morsy, et al, 2019). The main drawbacks of physical and chemical methods for synthesizing nanomaterials are using toxic compounds, consuming a high amount of energy, and generating hazardous wastes.…”

Section: Introductionmentioning

confidence: 99%

Anthelmintic and hepatoprotective activities of the biocompatible Zinc Oxide Nanoparticles against Parascaris equorum infection in rats

Ali

Mohamed

Fahmy

et al. 2022

Preprint

Self Cite

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Background: Nanoparticles have many properties, depending on their size, shape, and morphology, allowing them to interact with microorganisms, plants, and animals. Objectives: Investigatation of the therapeutic effects biocompatible zinc oxide nanoparticles (ZnO NPs) on P. equorum infection in rats. Methods: Thirty-six rats were divided into two divisions are: the first division is noninfected groups were allocated into three groups. Group 1: Control, Group 2: ZnO NPs (30mg/kg) and group 3: ZnO NPs (60mg/kg). The second division is infected groups were allocated into three groups. Group 1: vehicle, group 2: ZnO NPs (30mg/kg) and group 3: ZnO NPs (60mg/kg). Findings: Biocompatible ZnO NPs caused a significant decrease in liver functions, LDL, cholesterol, triglycerides, MDA and NO. While it caused a significant increase in HB, HDL, BCHE, GSH, CAT, and GST in infected treated rats. The histological inflammation and fibroplasia scores showed a significant enhancement during the treatment with ZnO NPs (30, 60mg/kg) compared to the infected untreated animals that scored the highest pathological destruction score. Immunohistochemical markers of NF-κB showed a significant decrease during the treatment with ZnO NPs (30, 60mg/kg) compared to the infected untreated animals. Main conclusions: Biocompatible Zinc Oxide Nanoparticle is a promising treatment modality against parasitic infection through its powerful anthelmintic, antioxidant, healing promotion, and anti-inflammation effects.

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Optimizing and Evaluating the Antihelminthic Activity of the Biocompatible Zinc Oxide Nanoparticles Against the Ascaridid Nematode, Parascaris equorum In Vitro

Cited by 16 publications

References 58 publications

Toxicities of the copper and zinc oxide nanoparticles onMarshallagia marshalli(Nematoda: Trichostrongylidae): evidence on oxidative/nitrosative stress biomarkers, DNA damage and egg hatchability

Toxicities of the copper and zinc oxide nanoparticles onMarshallagia marshalli(Nematoda: Trichostrongylidae): evidence on oxidative/nitrosative stress biomarkers, DNA damage and egg hatchability

The equine ascarids: resuscitating historic model organisms for modern purposes

Anthelmintic and hepatoprotective activities of the biocompatible Zinc Oxide Nanoparticles against Parascaris equorum infection in rats

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