Optimizing amino acid balance in fish meal‐free diets for GIFT strain of Nile tilapia ( Oreochromis niloticus ) by deletion method

Abstract

An indoor feeding trial was conducted to optimize the amino acid (AA) profile of the genetically improved farmed tilapia (GIFT) diet using the AA deletion method. Six corn–soy‐based isonitrogenous and isoenergetic fishmeal‐free diets with different AA profiles were formulated to evaluate the effects of crystalline AA supplementation on the growth performance, whole‐body composition, muscle growth‐related gene expression and haemato‐biochemical responses of juvenile GIFT tilapia. Each diet was randomly assigned to triplicate groups of 30 fish (5.5 ± 0.3 g) per experimental unit, which were fed three times a day (09:00, 13:00 and 17:00 h) for a period of 60 days. Maximum growth performances and feed utilization were observed in fish fed a balanced diet and a ‐Trp diet compared with the AA‐unsupplemented diet, whereas no significant differences were observed among fish fed –Met, –Lys and –Arg diets. Whole‐body proximate composition and amino acid profile of GIFT tilapia were not significantly affected by diets with different amino acid profiles. Similarly, no significant differences were observed in blood biochemical responses, except glucose. However, significant (p < .05) differences were observed in haematological responses and mRNA expression of MyoD,myogenin and myostatin. Based on the growth performance and feed efficiency, the EAA pattern associated with –Trp diet was considered to be the optimum EAA ratio for maximum growth and feed utilization of juvenile GIFT tilapia.

Dietary Supplementation of Crystalline Amino Acid Improves Growth Performance and Health of Yellow Catfish That Reduced by Plant Proteins Replacement of Fishmeal

“…In this study, WG, SGR, FCR, and SUR of yellow catfish were similar to those of the control group after balanced amino acid supplementation in the 25%PP group (25%PP + AA). This suggests that a balanced intake of amino acids is necessary for the growth performance of fish [31,32]. In this study, the CP content of muscle in the 25%PP group was higher than Con and 25%PP + AA groups; this suggests that unbalanced amino acid intake can reduce muscle crude protein content, which recovered to control levels after CAA supplementation, suggesting that balanced amino acid intake is crucial for muscle protein accumulation.…”

Using Azadirachta indica protein hydrolysate as a plant protein in Nile tilapia (Oreochromis niloticus) diet: Effects on the growth, economic efficiency, antioxidant‐immune response and resistance to Streptococcus agalactiae

“…Together with AAs, these peptides can promote protein synthesis, support growth and offer nutritional significance (Barakat & Ghazal, 2016;Habte-Tsion, 2020). Hence, the AAs composition of AIPH-included diets used in the current work seems sufficient and meets the needs of O. niloticus (Nguyen et al, 2020;Prabu et al, 2021) that reflects on the fish growth.…”

Coated crystalline essential amino acid supplementation supports high soybean meal inclusion in diets for Nile tilapia (Oreochromis niloticus) with improved protein retention and nutrient assimilation

“…He et al ., (2013) established the methionine and lysine requirements for maintenance and efficiency of utilisation for growth of two sizes of tilapia ( Oreochromis niloticus ). Prabu et al ., (2021) also worked on tilapia to examine the supplementation of key crystalline amino acids, namely methionine, lysine, tryptophan, and arginine. These researchers used a high soybean meal formulation (analogous to the current study) to assess the respective essential amino acid requirements by their individual deletion from the reference diet.…”