Optimizing a CRISPR-Cas13d gene circuit for tunable target RNA downregulation with minimal collateral RNA cutting
Yiming Wan,
Damiano Coraci,
Christopher Helenek
et al.
Abstract:The invention of RNA-guided DNA cutting systems has revolutionized biotechnology. More recently, RNA-guided RNA cutting by Cas13d entered the scene as a highly promising alternative to RNA interference to engineer cellular transcriptomes for biotechnological and therapeutic purposes. Unfortunately, collateral damage by indiscriminate off-target cutting tampered enthusiasm for these systems. Yet, how collateral damage, or even RNA target reduction depends on Cas13d and guide RNA abundance has remained unclear d… Show more
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