Optimized transitory ectopic expression of promastigote surface antigen protein in Nicotiana benthamiana, a potential anti-leishmaniasis vaccine candidate

Gene-silencing suppressors for high-level production of the HIV-1 entry inhibitor griffithsin in Nicotiana benthamiana

“…P0 protein is a silencing suppressor which inhibits local and systemic RNA silencing through AGO1 degradation [60]. Our result is similar to that of Lacombe et al [61], who recently showed the effect of the combination of P19, P1 and P0 to enhance expression levels of anti-leishmaniasis vaccine candidate by transient expression system in N. benthamiana. The synergetic effect of P0 and P1 on RNA silencing suppression was shown in combination with P19.…”

“…It is hypothesized that the P1suppressor could affect system RNA silencing suppression via inhibition of 24 nts siRNA production. As 24 nts siRNA might induce the formation of systemic RNA silencing signal and the combination of the P1 suppressor with P19 and P0 would strongly affect the RNA gene silencing, and consequently increase recombinant protein production in plant not only locally but also on systemic level through viral based tool [61]. Based on our data, we can speculate that combination of P19, P0, and P1 suppressors could be efficiently applied to boost expression level of other recombinant protein by transient expression in N. benthamiana.…”

“…Based on our data, we can speculate that combination of P19, P0, and P1 suppressors could be efficiently applied to boost expression level of other recombinant protein by transient expression in N. benthamiana. However, the use of the three P19, P0, and P1 gene suppressors together might not be recommended for stable transgenic plants since the combination of gene silencing suppressors could trigger harmful developmental damage on plant cell [61,62].…”

“…RNA silencing suppression activity of viral protein of virus stems from its ability to bind siRNA as well as proteins involved in antiviral silencing ( [42,43]. The P19 suppressor from the Tomato bushy stunt virus (TBSV) has been widely used to boost transient or constitutive expression level of recombinant proteins in the plant as it selectively inhibits the 21-nt and 22-nt classes of siRNA and also reduce the availability of free siRNA for RISC loading [58,61].…”

Nicotiana benthamiana is a suitable transient system for high-level expression of an active inhibitor of cotton boll weevil α-amylase

“…Indeed, the presence of such viral proteins in transient expression systems allows overcoming the gene silencing triggered by the plant defence machinery to specifically degrade foreign nucleic acids. Consequently, the yield of the protein of interest is dramatically increased by 50 fold or more [30, 31].…”