Optimized retinal nerve fiber layer segmentation based on optical reflectivity and birefringence for polarization-sensitive optical coherence tomography

Wang, Bingqing; Paranjape, Amit S.; Yin, Biwei; Liu, Shuang; Markey, Mia K.; Milner, Thomas E.; Rylander, H. G.

doi:10.1117/12.892826

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…A LABVIEW software program (National Instruments, Austin, Texas) was implemented for the OCT system to automatically detect RNFL boundaries in each B-scan of continuous ring scans [ 25 , 26 ]. After RNFL boundary detection, an expert on OCT retinal image evaluation visually inspected the boundaries overlaid on each B-scan to correct any misidentified boundaries.…”

Section: Methodsmentioning

confidence: 99%

Effect of image registration on longitudinal analysis of retinal nerve fiber layer thickness of non-human primates using Optical Coherence Tomography (OCT)

Liu

Datta

et al. 2015

Eye and Vis

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BackgroundIn this paper we determined the benefits of image registration on estimating longitudinal retinal nerve fiber layer thickness (RNFLT) changes.MethodsRNFLT maps around the optic nerve head (ONH) of healthy primate eyes were measured using Optical Coherence Tomography (OCT) weekly for 30 weeks. One automatic algorithm based on mutual information (MI) and the other semi-automatic algorithm based on log-polar transform cross-correlation using manually segmented blood vessels (LPCC_MSBV), were used to register retinal maps longitudinally. We compared the precision and recall between manually segmented image pairs for the two algorithms using a linear mixed effects model.ResultsWe found that the precision calculated between manually segmented image pairs following registration by LPCC_MSBV algorithm is significantly better than the one following registration by MI algorithm (p < <0.0001). Trend of the all-rings and temporal, superior, nasal and inferior (TSNI) quadrants average of RNFLT over time in healthy primate eyes are not affected by registration. RNFLT of clock hours 1, 2, and 10 showed significant change over 30 weeks (p = 0.0058, 0.0054, and 0.0298 for clock hours 1, 2 and 10 respectively) without registration, but stayed constant over time with registration.ConclusionsThe LPCC_MSBV provides better registration of RNFLT maps recorded on different dates than the automatic MI algorithm. Registration of RNFLT maps can improve clinical analysis of glaucoma progression.Electronic supplementary materialThe online version of this article (doi:10.1186/s40662-015-0013-7) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Effect of image registration on longitudinal analysis of retinal nerve fiber layer thickness of non-human primates using Optical Coherence Tomography (OCT)

Liu

Datta

et al. 2015

Eye and Vis

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“…17 A-scans in each cluster were averaged to reduce speckle noise, with the assumption that RNFL boundaries are changing slowly in each small cluster. A thresholding procedure was applied on these averaged A-scans to provide an initial guess of the vitreous-RNFL boundary location.…”

Section: Rnfl Thickness Measurementsmentioning

confidence: 99%

Thickness, Phase Retardation, Birefringence, and Reflectance of the Retinal Nerve Fiber Layer in Normal and Glaucomatous Non-Human Primates

Dwelle

Liu

Wang

et al. 2012

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. We identified candidate optical coherence tomography (OCT) markers for early glaucoma diagnosis. Time variation of retinal nerve fiber layer (RNFL) thickness, phase retardation, birefringence, and reflectance using polarization sensitive optical coherence tomography (PS-OCT) were measured in three non-human primates with induced glaucoma in one eye. We characterized time variation of RNFL thickness, phase retardation, birefringence, and reflectance with elevated intraocular pressure (IOP). METHODS.One eye of each of three non-human primates was laser treated to increase IOP. Each primate was followed for a 30-week period. PS-OCT measurements were recorded at weekly intervals. Reflectance index (RI) is introduced to characterize RNFL reflectance. Associations between elevated IOP and RNFL thickness, phase retardation, birefringence, and reflectance were characterized in seven regions (entire retina, inner and outer rings, and nasal, temporal, superior and inferior quadrants) by linear and non-linear mixed-effects models.RESULTS. Elevated IOP was achieved in three non-human primate eyes with an average increase of 13 mm Hg over the study period. Elevated IOP was associated with decreased RNFL thickness in the nasal region (P ¼ 0.0002), decreased RNFL phase retardation in the superior (P ¼ 0.046) and inferior (P ¼ 0.021) regions, decreased RNFL birefringence in the nasal (P ¼ 0.002) and inferior (P ¼ 0.029) regions, and loss of RNFL reflectance in the outer rings (P ¼ 0.018). When averaged over the entire retinal area, only RNFL reflectance showed a significant decrease (P ¼ 0.028).CONCLUSIONS. Of the measured parameters, decreased RNFL reflectance was the most robust correlate with glaucomatous damage. Candidate cellular mechanisms are considered for decreased RNFL reflectance, including mitochondrial dysfunction and retinal ganglion cell apoptosis. (Invest Ophthalmol Vis Sci. 2012;53:4380-4395) DOI:10.1167/iovs.11-9130 G laucoma is an optic neuropathy characterized by the progressive death of retinal ganglion cells and loss of associated axons in the retinal nerve fiber layer (RNFL). Without early diagnosis and therapeutic intervention, glaucoma eventually results in visual field abnormalities and vision loss. Studies indicate that as many as 40% of the axons may die before the visual field is affected, 1 suggesting an opportunity for the development of diagnostic tools that can prevent visual field loss in conjunction with application of neuroprotective strategies. As axons in the RNFL are lost, an associated RNFL thinning is observed. 2-4Optical coherence tomography (OCT) is an imaging modality that provides high-resolution (2-15 lm) cross-sectional images of the retina at video rate, and can quantify macroscopic changes in the optic nerve head (ONH) and RNFL. Inasmuch as previous studies have demonstrated a link between decreased RNFL thickness measured with OCT and glaucoma, 3 OCT measurements of RNFL thickness have become a routine component of glaucoma screening, diagnosis, and monitoring protocols...

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Optimized retinal nerve fiber layer segmentation based on optical reflectivity and birefringence for polarization-sensitive optical coherence tomography

Cited by 2 publications

References 21 publications

Effect of image registration on longitudinal analysis of retinal nerve fiber layer thickness of non-human primates using Optical Coherence Tomography (OCT)

Effect of image registration on longitudinal analysis of retinal nerve fiber layer thickness of non-human primates using Optical Coherence Tomography (OCT)

Thickness, Phase Retardation, Birefringence, and Reflectance of the Retinal Nerve Fiber Layer in Normal and Glaucomatous Non-Human Primates

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