Optimized purification process of polysaccharides from Carex meyeriana Kunth by macroporous resin, its characterization and immunomodulatory activity

Hu, Zhengyu; Zhou, Hongli; Li, Yaping; Wu, Meifu; Yu, Ming; Sun, Xinshun

doi:10.1016/j.ijbiomac.2019.03.207

Cited by 63 publications

(45 citation statements)

References 54 publications

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“…A series of dextran standards with different molecular weights were used to establish calibration curve. The analytical conditions were: Ultrapure water was served as mobile phase at a flow rate of 1.0 mL/min; the temperatures of column and RID were set as 50 and 35°C, respectively and the run rate was set as 30 min (Hu et al, 2019).…”

Section: Molecular Weightmentioning

confidence: 99%

“…The analytical conditions were: the mobile phase consisted of PBS (pH 6.8) and acetonitrile (82:18, v/v) at a flow rate of 0.8 mL/min; column temperature-30°C; detector wavelength-245 nm; run rate-85 min. Different commercial monosaccharides were served as standards (Hu et al, 2019).…”

Section: Monosaccharide Compositionsmentioning

confidence: 99%

“…Effects of POR-3 on the proliferation of RAW264.7 cells were estimated by MTT method with some modifications (Hu et al, 2019). Accordingly, RAW264.7 cells were seeded in 96-well plates containing RPMI-1640 medium and 10% FBS.…”

Section: The In Vitro Immunomodulatory Activity Of Por-3 Macrophages mentioning

confidence: 99%

“…Effects of POR-3 on the macrophages phagocytosis were evaluated by the neutral red uptake method with minor modifications (Hu et al, 2019). In brief, RAW264.7 cells were seeded in 96-well plates and exposed to different concentrations of POR-3 solution or complete medium alone (negative control) or LPS solution (positive control) and incubated for 24 h. After that, the supernatant was transferred into another 96well plate for the determination of NO content (see section of NO assay) and non-adherent cells were removed by washing twice with PBS, followed by adding 100 μL of neutral red solution to each well and by being incubated for 1 h. After removal of the supernatant and excessive neutral red solution, cells were washed twice with PBS.…”

Section: Macrophages Phagocytosis Assaymentioning

confidence: 99%

“…Effects of POR-3 on NO release were appraised according to the method described in NO assay kit (Hu et al, 2019). In short, the supernatant was mixed with equivalent amounts of Griess reagent I (1% sulfanilamide) and Griess reagent II (0.1% N-1naphthylethylenediamine dihydrochloride in 5% phosphoric acid) at room temperature for 10 min.…”

Section: No Assaymentioning

confidence: 99%

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A Polysaccharide-Rich Fraction Derived from Oviductus ranae with Antioxidant and Immunomodulatory Potentials

Yu¹,

Wang²,

Liu³

et al. 2020

American Journal of Biochemistry and Biotechnology

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Section: Molecular Weightmentioning

confidence: 99%

Section: Monosaccharide Compositionsmentioning

confidence: 99%

Section: The In Vitro Immunomodulatory Activity Of Por-3 Macrophages mentioning

confidence: 99%

Section: Macrophages Phagocytosis Assaymentioning

confidence: 99%

Section: No Assaymentioning

confidence: 99%

See 3 more Smart Citations

A Polysaccharide-Rich Fraction Derived from Oviductus ranae with Antioxidant and Immunomodulatory Potentials

Yu¹,

Wang²,

Liu³

et al. 2020

American Journal of Biochemistry and Biotechnology

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Microwave‐Assisted Enzymatic Extraction, Partial Characterization, and Antioxidant Potential of Polysaccharides from Sagittaria trifolia Tuber

Zhang

Wang

Yang

et al. 2022

Chemistry & Biodiversity

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Sagittaria trifolia tuber is an aquatic vegetable. In this work, microwave‐assisted enzymatic extraction (MEE) was used to extract S. trifolia tuber polysaccharides (STTPs). Optimum conditions were complex enzyme of 2 %, liquid‐to‐solid ratio of 43 : 1 mL g−1, microwave power of 506 W, and time of 8 min, under which STTPs yield was 36.22±0.69 %, higher than those of other methods. STTPs were sulfated polysaccharides with sulfur valence of S6+. STTPs comprised mannose, glucose, galactose, and arabinose at a mole ratio of 3.69 : 19.33 : 6.21 : 1.00, molecular weights of 3606 kDa and 149.6 kDa, particle size of 220 nm, and zeta potential of −5.02 mV. The surface of STTPs was full of bumps and holes, and abundant in O1s and non‐functionalized C1s. STTPs would scavenge reactive oxygen species with advantage. It would provide an efficient MEE method to obtain antioxidant STTPs, also a clue for extracting polysaccharides from starch‐rich crops.

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Isolation and Structural Characterization of Two Polysaccharides from Dracocephalum moldavica and Their Anti‐Complementary Activity

Wang

Jin

et al. 2022

Chemistry & Biodiversity

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The two novel polysaccharides, DMP‐1 and DMP‐2, with molecular weights of 4.1553×105 kDa and 1.9764×105 kDa, respectively, were isolated from Dracocephalum moldavica. The structural characterization indicated that DMP‐1 and DMP‐2 shared a similar backbone consisting of →5)‐Araf‐(1→, Manp‐(1→, Glcp‐(1→, →2)‐Manp‐(1→, →6)‐Glcp‐(1→ and →3,6)‐Galp‐(1→ with a different molar ratios and triple‐helix structures with α‐ and β‐type glycosidic bonds. The anti‐complementary activity evaluation showed that DMP‐1 and DMP‐2 had strong complement inhibition through the classical pathway (CP), alternative pathway (AP) and lectin pathway (LP). Mechanistic studies indicated that DMP‐1 can block the activation cascade of the complement system by targeting C2, C3, C5, C9, Factor B and Factor P, and that DMP‐2 inhibited complement activation by blocking C2, C3, C4, C5, C9 and Factor B.

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Optimized purification process of polysaccharides from Carex meyeriana Kunth by macroporous resin, its characterization and immunomodulatory activity

Cited by 63 publications

References 54 publications

A Polysaccharide-Rich Fraction Derived from Oviductus ranae with Antioxidant and Immunomodulatory Potentials

A Polysaccharide-Rich Fraction Derived from Oviductus ranae with Antioxidant and Immunomodulatory Potentials

Microwave‐Assisted Enzymatic Extraction, Partial Characterization, and Antioxidant Potential of Polysaccharides from Sagittaria trifolia Tuber

Isolation and Structural Characterization of Two Polysaccharides from Dracocephalum moldavica and Their Anti‐Complementary Activity

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