Optimized polydopamine coating and DNA conjugation onto gold nanorods for single nanoparticle bioaffinity measurements

Azab, Marwa M.; Cherif, Rédha; Finnie, Aryanne L.; El‐Alamin, Maha M. Abou; Sultan, M. S.; Wark, Alastair W.

doi:10.1039/c7an02019h

Cited by 14 publications

(11 citation statements)

References 54 publications

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“…In addition, the introduction of PDA also prolonged the blood circulation time of gold nanorods, where the half-life increased from 0.7 to 4.5 h. Cu(II) was doped in the PDA layer, which endowed gold nanorods with MRI and chemotherapeutic functions. In a separate study, Azab et al explored the growth of PDA layer on positively or negatively charged gold nanorod surfaces . They studied the effect of different functional groups such as polyelectrolyte and alkanethiol deposited onto gold nanorod surfaces on the kinetics and thickness of the PDA layer.…”

Section: Surface Modificationmentioning

confidence: 99%

Versatile Polydopamine Platforms: Synthesis and Promising Applications for Surface Modification and Advanced Nanomedicine

et al. 2019

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As a mussel-inspired material, polydopamine (PDA), possesses many properties, such as a simple preparation process, good biocompatibility, strong adhesive property, easy functionalization, outstanding photothermal conversion efficiency, and strong quenching effect. PDA has attracted increasingly considerable attention because it provides a simple and versatile approach to functionalize material surfaces for obtaining a variety of multifunctional nanomaterials. In this review, recent significant research developments of PDA including its synthesis and polymerization mechanism, physicochemical properties, different nano/microstructures, and diverse applications are summarized and discussed. For the sections of its applications in surface modification and biomedicine, we mainly highlight the achievements in the past few years (2016–2019). The remaining challenges and future perspectives of PDA-based nanoplatforms are discussed rationally at the end. This timely and overall review should be desirable for a wide range of scientists and facilitate further development of surface coating methods and the production of PDA-based materials.

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Section: Surface Modificationmentioning

confidence: 99%

Versatile Polydopamine Platforms: Synthesis and Promising Applications for Surface Modification and Advanced Nanomedicine

et al. 2019

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“…Similarly, NaCl was then added to 0.15 m and allowed to react for another day. [112] DNA strands can also be attached to PDA coated probes and films using similar methods. [113][114][115] As described above, adding NaCl followed by incubation (salt aging) was performed to reach a high DNA density.…”

Section: Covalent Dna Attachmentmentioning

confidence: 99%

“…[99,107] Azab et al found a 45-50% decrease in surface density when the saltaging method was not used. [112] In addition to charge screening, an alkane, PEG, or polynucleotide spacer inserted between the amine/thiol functional group and the end of DNA could further increase the DNA packing density by reducing steric interactions and intermolecular repulsion of adjacent DNA strands at the surface of small NPs. [112,116] Figure 6.…”

Section: Covalent Dna Attachmentmentioning

confidence: 99%

“…[112] In addition to charge screening, an alkane, PEG, or polynucleotide spacer inserted between the amine/thiol functional group and the end of DNA could further increase the DNA packing density by reducing steric interactions and intermolecular repulsion of adjacent DNA strands at the surface of small NPs. [112,116] Figure 6. Reaction mechanisms for thiol or amine modified DNA relying on the dopamine-quinone moieties within the PDA structure.…”

Section: Covalent Dna Attachmentmentioning

confidence: 99%

“…Similarly, NaCl was then added to 0.15 m and allowed to react for another day. [ 112 ] DNA strands can also be attached to PDA coated probes and films using similar methods. [ 113–115 ]…”

Section: Dna Adsorption By Pdamentioning

confidence: 99%

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Interfacing DNA and Polydopamine Nanoparticles and Its Applications

Zandieh

Hagar

Liu

2020

Part & Part Syst Charact

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and drugs, [21,22] and decrease the toxicity of biomaterials. [23] Finally, PDA can be used for sensing. PDA-containing biosensors have been used to detect various organic molecules, [24,25] biomolecules, [26,27] and metal ions. [28] Many sensing and biomedical applications of PDA have been realized through interfacing it with biomolecules such as DNA, enzymes, and antibodies. DNA has gained popularity in the last few decades as a functional polymer thanks to its high stability, low cost, and ease of synthesis. [29-34] More importantly, DNA can bind not only its complementary sequence but also a diverse range of other molecules by using DNA aptamers. [35] Catalytically active DNA sequences, known as DNAzymes, have also been discovered. [36] Combining the unique properties of PDA and DNA has produced many interesting hybrid materials. The surface of PDA is different from most inorganic and even other organic NPs, and understanding its surface interactions is critical for applications. Given the many papers on DNA-functionalized PDA which have been published, it is now a good time to summarize this topic, which was not previously reviewed. This review has two main purposes. First, it describes the interfacial interactions between DNA and PDA with a focus on methods to achieve noncovalent and covalent conjugates. Second, we review the representative applications of such conjugates. In the end, some challenges of the field are discussed, and a few future research directions are proposed. 2. Dopamine, Its Analogs, and PDA The extraordinary wet adhesion of some marine creatures, such as mussels, is caused by their secreted proteins that are rich in catecholamine groups. This has inspired scientists to develop a universal coating material that can mimic the natural wet adhesion. [37] Although dopamine is a catecholamine, PDA is not yet found naturally. PDA is a synthetic analog of melanin, and natural melanin exists in the color pigments of the skin, hair, and eyes of the human body. Melanin is produced by the aggregation and polymerization of catecholamines such as DOPA or tyrosine (Figure 1A). [38,39] To date, the polymerization pathways of dopamine are still unknown, but a few likely pathways have been proposed. [40-44] Polydopamine (PDA) is polymerized from dopamine under oxidative and basic conditions. PDA is a biocompatible material with great versatility for coating various surfaces and it can also form nanoparticles. DNA oligonucleotides are highly stable, and they can recognize a diverse range of molecules from complementary nucleic acids, proteins to small molecules and metal ions. To enhance the molecular recognition function of PDA, it is interfaced with DNA and the conjugates have achieved a wide range of applications in biomedical and analytical science. In this review, the chemistry of some catecholamines, including dopamine and PDA, is first briefly introduced and variables in the PDA synthesis are highlighted. Strategies to promote DNA adsorption on PDA are then discussed including the use of low pH and...

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Mg2+-promoted high-efficiency DNA conjugation on polydopamine surfaces for aptamer-based ochratoxin A detection

Yang,

Feng

2024

Analytica Chimica Acta

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Optimized polydopamine coating and DNA conjugation onto gold nanorods for single nanoparticle bioaffinity measurements

Cited by 14 publications

References 54 publications

Versatile Polydopamine Platforms: Synthesis and Promising Applications for Surface Modification and Advanced Nanomedicine

Versatile Polydopamine Platforms: Synthesis and Promising Applications for Surface Modification and Advanced Nanomedicine

Interfacing DNA and Polydopamine Nanoparticles and Its Applications

Mg2+-promoted high-efficiency DNA conjugation on polydopamine surfaces for aptamer-based ochratoxin A detection

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