Optimized PAH/Folic acid layer-by-layer films as an electrochemical biosensor for the detection of folate receptors

Correia, Abilene Rodrigues; Sampaio, Isabella; Comparetti, Edson José; Vieira, Nirton Cristi Silva; Zucolotto, Valtencir

doi:10.1016/j.bioelechem.2020.107685

Cited by 12 publications

(7 citation statements)

References 40 publications

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“…FRs have been detected in tissue samples using different techniques, such as surface enhanced Raman spectroscopy (SERS) [16], proteomics assays [17], fluorescence imaging [18,19], and positron emission tomography (PET) [20,21]. However, these techniques are tedious, costly, time consuming, pose a risk to human health due to toxic radioligands, and have low sensitivity, such as FA-fluorescein isothiocyanate dye, which has a short systemic circulation time, and therefore does not target tumors efficiently [22].…”

Section: Introductionmentioning

confidence: 99%

“…In particular, electrochemical impedance spectroscopy (EIS) is a powerful label-free technique that can directly determine biomolecular recognition actions and the appropriate modification of sensitive elements on the electrode surface [25,26]. Compared to proteins, peptides, and antibodies, folic acid (FA) has a high affinity for binding FR with a K d ~10 −9 M [22,27]. This affinity is due to the presence of specific binding sites on the FA molecule that are complementary to the structure of FRs.…”

Section: Introductionmentioning

confidence: 99%

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Impedimetric Detection of Cancer Markers Based on Nanofiber Copolymers

Elnagar,

Elgiddawy,

El Rouby

et al. 2024

Biosensors

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The sensitive determination of folate receptors (FRs) in the early stages of cancer is of great significance for controlling the progression of cancerous cells. Many folic acid (FA)-based electrochemical biosensors have been utilized to detect FRs with promising performances, but most were complicated, non-reproducible, non-biocompatible, and time and cost consuming. Here, we developed an environmentally friendly and sensitive biosensor for FR detection. We proposed an electrochemical impedimetric biosensor formed by nanofibers (NFs) of bio-copolymers prepared by electrospinning. The biosensor combines the advantages of bio-friendly polymers, such as sodium alginate (SA) and polyethylene oxide (PEO) as an antifouling polymer, with FA as a biorecognition element. The NF nanocomposites were characterized using various techniques, including SEM, FTIR, zeta potential (ZP), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). We evaluated the performance of the NF biosensor using EIS and demonstrated FR detection in plasma with a limit of detection of 3 pM. Furthermore, the biosensor showed high selectivity, reliability, and good stability when stored for two months. This biosensor was constructed from ‘green credentials’ holding polymers that are highly needed in the new paradigm shift in the medical industry.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Impedimetric Detection of Cancer Markers Based on Nanofiber Copolymers

Elnagar,

Elgiddawy,

El Rouby

et al. 2024

Biosensors

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“…Collagen, with a triple-helix structure composed of α-chain peptides, is one of the important rich substances in the extracellular matrix of vertebrates and plays an important role in the integrity and mechanical properties of biological tissues [ 16 , 17 , 18 , 19 , 20 ]. The network structure of collagen in cartilage is the foundation of the joint structure, which provides the joint with a certain degree of flexibility and stability.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Gelatin-Quaternary Ammonium Salt Coating on Titanium Surface for Antibacterial/Osteogenic Properties

Song,

Xing,

Wei

et al. 2023

Molecules

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Titanium (Ti) and its alloys are widely used in medical treatment, engineering, and other fields because of their excellent properties including biological activity, an elastic modulus similar to that of human bones, and corrosion resistance. However, there are still many defects in the surface properties of Ti in practical applications. For example, the biocompatibility of Ti with bone tissue can be greatly reduced in implants due to a lack of osseointegration as well as antibacterial properties, which may lead to osseointegration failure. To address these problems and to take advantage of the amphoteric polyelectrolyte properties of gelatin, a thin layer of gelatin was prepared by electrostatic self-assembly technology. Diepoxide quaternary ammonium salt (DEQAS) and maleopimaric acid quaternary ammonium salt (MPA−N+) were then synthesized and grafted onto the thin layer. The cell adhesion and migration experiments demonstrated that the coating has excellent biocompatibility, and those grafted with MPA−N+ promoted cell migration. The bacteriostatic experiment showed that the mixed grafting with two ammonium salts had excellent bacteriostatic performance against Escherichia coli and Staphylococcus aureus, with bacteriostasis rates of 98.1 ± 1.0% and 99.2 ± 0.5%, respectively.

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“…The expression of FRs is limited in normal tissues. However, the overexpression of FRs has been demonstrated in tumor cells, including ovarian, lung, cervical carcinoma, gastric, prostate cancer, and so on [ 7 , 35 ]. The difference in FR expression, along with the high affinity between FRs and FA, has broadened the way towards FA-related targeting therapeutics and cancer cell analysis.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Cytosensor Based on a Gold Nanostar-Decorated Graphene Oxide Platform for Gastric Cancer Cell Detection

Zhang

Liu

Huang

et al. 2022

Sensors

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Effectively capturing and sensitively detecting cancer cells are critical to clinical diagnosis and cancer therapy. In this work, we prepared gold nanostar-decorated graphene oxide (GO-AuNSs) nanocomposites using a ultraviolet (UV)-induced strategy, and then modified them with a layer of bio-complex rBSA-FA (coupled reduced bovine serum albumin with folic acid) to generate GO-AuNSs@rBSA-FA nanocomposites. Herein, the application of GO and AuNSs not only strengthened the conductivity of the sensing platform but also guaranteed nanocomposites with biocompatible performance. Moreover, the adopted rBSA-FA layer could effectively enhance the stability and specificity towards gastric cancer cells (MGC-803). According to a systemic construction procedure, a novel electrochemical cytosensor based on GO-AuNSs@rBSA-FA was fabricated for MGC-803 cell detection. With the assistance of cyclic voltammetry (CV) and differential pulse voltammetry (DPV), the cytosensor reached a detection limit of 100 cell/mL in a wide linear range of 3 × 102~7 × 106 cell/mL towards MGC-803 cells. The good electrochemical characteristics for the cancer cell analysis indicate a promising prospect of this electrochemical cytosensor in clinical cancer diagnosis.

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Optimized PAH/Folic acid layer-by-layer films as an electrochemical biosensor for the detection of folate receptors

Cited by 12 publications

References 40 publications

Impedimetric Detection of Cancer Markers Based on Nanofiber Copolymers

Impedimetric Detection of Cancer Markers Based on Nanofiber Copolymers

Preparation of Gelatin-Quaternary Ammonium Salt Coating on Titanium Surface for Antibacterial/Osteogenic Properties

Electrochemical Cytosensor Based on a Gold Nanostar-Decorated Graphene Oxide Platform for Gastric Cancer Cell Detection

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